LOCUS       sequence-33315-e         868 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Kim JH, Wei JR, Wallach JB, Robbins RS, Rubin EJ, Schnappinger D
  TITLE     Protein inactivation in mycobacteria by controlled proteolysis and 
            its application to deplete the beta subunit of RNA polymerase.
  JOURNAL   Nucleic Acids Res. 2011 Mar;39(6):2210-20. doi: 10.1093/nar/gkq1149
  PUBMED    21075796
REFERENCE   2  (bases 1 to 868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2011-03"; volume: "39"; issue: "6"; 
            pages: "2210-20"
COMMENT     Sequence Label: pGMEH-P38-che-ssrAec3
FEATURES             Location/Qualifiers
     source          1..868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(284..304)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(312..329)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(312..328)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(321..343)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gtacccggag gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg
       61 cggccactac gacgcggagg tcaagaccac ctacaaggcc aagaagccgg tgcagctgcc
      121 gggcgcctac aacgtcaaca tcaagttgga catcacctcg cacaacgagg actacaccat
      181 cgtggaacag tacgaacgcg ccgagggccg ccactcgacc ggcggtatgg acgagctgta
      241 caaggccgcg aacgacgaga actacgcact ggccgcgtag tgacaacttt attatacata
      301 gttgataatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
      361 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
      421 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg
      481 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggggatt ggtggcgacg
      541 actcctggag cccgtcagta tcggcggaat tccagctgag cgccggtcgc taccattacc
      601 agttggtctg gtgtcaaaaa taataataac cgggcaggcc atgtctgccc gtatttcgcg
      661 taaggaaatc cattatgtac tatttaaaaa acacaaactt ttggatgttc ggtttattct
      721 ttttctttta cttttttatc atgggagcct acttcccgtt tttcccgatt tggctacatg
      781 acatcaacca tatcagcaaa agtgatacgg gtattatttt tgccgctatt tctctgttct
      841 cgctattatt ccaaccgctg tttggtct
//
