LOCUS       sequence-33316-e         855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Kim JH, Wei JR, Wallach JB, Robbins RS, Rubin EJ, Schnappinger D
  TITLE     Protein inactivation in mycobacteria by controlled proteolysis and 
            its application to deplete the beta subunit of RNA polymerase.
  JOURNAL   Nucleic Acids Res. 2011 Mar;39(6):2210-20. doi: 10.1093/nar/gkq1149
  PUBMED    21075796
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2011-03"; volume: "39"; issue: "6"; 
            pages: "2210-20"
COMMENT     Sequence Label: pGMEH-P38-che-DAS+4
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    134..158
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(650..674)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(678..698)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(718..734)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(718..734)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(731..753)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcgccctcgc cctcgatctc gaactcgtgg ccgttcacgg agccctccat gtgcaccttg
       61 aagcgcatga actccttgat gatggccatg ttgtcctcct cgcccttgct caccattatt
      121 aacctcctgc atgcccactt tgtacaagaa agctgggtcg gatcaacggc ctggtcgaca
      181 agggcaatac ggtgatcgtc atcgaacata acctggacgt gatcggccgt caactcgttg
      241 aggtaccggt cggattcaaa tggttcgtcg acggtctgat cccgcggtgt gcaccgatgc
      301 cctcacggtt ccagcggtcg accaggtcct cgccgtgacg ccccttgatc gcaaacgcca
      361 ccgaatgcac cacggccaca gccaaagccg acacgctggt taccacgacg atgttcacgg
      421 ggggccctcg cggatctggc ccaactgctg gacttccacc gaagcaatcg agccgatgtc
      481 acgctgcaac tggtgcgggt gggcgacccg cgggcattcg gctgcgtacc caccgacgag
      541 gaggaccgcg tagtcgcctt tctggngaag acggagcaga cggtgctgtt gggcgcctgc
      601 tggattccca ggtcttcgaa caccgacacg ctcgcggcgt gatccaataa gcctgctttt
      661 ttgtacaaac ttgaattcaa cttttctata caaagttgat agcttggcgt aatcatggtc
      721 atagctgttt cctgtgtgaa attgttatcc gctggtatca gctcactcaa aggcggtaat
      781 acggttatcc acagaatcag gggataacgc aggaaagaac atggtgacct catgactcgc
      841 tagccagctg gggac
//
