LOCUS       sequence-33317-e         308 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Kim JH, Wei JR, Wallach JB, Robbins RS, Rubin EJ, Schnappinger D
  TITLE     Protein inactivation in mycobacteria by controlled proteolysis and 
            its application to deplete the beta subunit of RNA polymerase.
  JOURNAL   Nucleic Acids Res. 2011 Mar;39(6):2210-20. doi: 10.1093/nar/gkq1149
  PUBMED    21075796
REFERENCE   2  (bases 1 to 308)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2011-03"; volume: "39"; issue: "6"; 
            pages: "2210-20"
COMMENT     Sequence Label: pGMEH-P38-che-DAS+4
FEATURES             Location/Qualifiers
     source          1..308
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(278..302)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atccgcgagg gccccccgtg aacatcgtcg tggtaaccag cgtgtcngct ttggctgtgg
       61 ccgtggtgca ttcggtggcg tttgcgatca aggggcgtca cggcnaggac ctggtcgacc
      121 gctggaaccg tgagggcatc ggtgcacacc gcgggatcag accgtcnacg aaccatttga
      181 atccgaccgg tacctcaacg agttgacggc cgatcacntc caggttatgt tcgatgacga
      241 tcaccgtatt gcccttgtcg accaggccgt tgatccgacc cagctttctt gtacaaagtg
      301 ggcatgca
//
