LOCUS       sequence-33318-e         801 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Kim JH, Wei JR, Wallach JB, Robbins RS, Rubin EJ, Schnappinger D
  TITLE     Protein inactivation in mycobacteria by controlled proteolysis and 
            its application to deplete the beta subunit of RNA polymerase.
  JOURNAL   Nucleic Acids Res. 2011 Mar;39(6):2210-20. doi: 10.1093/nar/gkq1149
  PUBMED    21075796
REFERENCE   2  (bases 1 to 801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2011-03"; volume: "39"; issue: "6"; 
            pages: "2210-20"
COMMENT     Sequence Label: pGMEH-P38-che-DAS+4
FEATURES             Location/Qualifiers
     source          1..801
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(529..553)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(716..734)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 gaaaagttga attcaagttt gtacaaaaaa gcaggcttat tggatcacgc cgcgagcntg
       61 tnngtgttcg aagacctggg aatccagcag gcgcccaaca gcaccgtctg ctccgtcttc
      121 tccagaaagg cgactacgcg gtcctcctcg tcggtgggta cgcagccgaa tgcccgcggg
      181 tcgcccaccc gcaccagttg cagcgtgaca tcggctcgat tgcttcggtg gaagtccagc
      241 agttgggcca gatccgcgag ggccccccgt gaacatcgtc gtggtaacca gcgtgtcggc
      301 tttggctgtg gccgtggtgc attcggtggc gtttgcgatc aaggggcgtc acggcgagga
      361 cctggtcgac cgctggaacc gtgagggcat cggtgcacac cgcgggacca gaccgtcgac
      421 gaaccatttg aatccgaccg gtacctcaac gagttgacgg ccgatcacgt ccaggttatg
      481 ttcgatgacg atcaccgtat tgcccttgtc gaccaggccg ttgatccgac ccagctttct
      541 tgtacaaagt gggcatgcag gaggttaata atggtgagca agggcgagga ggacaacatg
      601 gccatcatca aggagttcat gcgcttcaag gtgcacatgg agggctccgt gaacggccac
      661 ganttcgaga tcgagggcga gggcgagggc cgcccgtacg agggcaccca gaccgccaag
      721 ctgaaggtga ccaagggtgg cccgctgccg ttcgcctggg acatcctgtc gccacagttc
      781 atgtacggct cgaaggtcta c
//
