LOCUS       sequence-333192-         834 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses in bacterial cytoplasm superfolder GFP with SpyTag003 and 
            flanking linkers between Asp173 and Gly174.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Keeble AH, Yadav VK, Ferla MP, Bauer CC, Chuntharpursat-Bon E, Huang
            J, Bon RS, Howarth M
  TITLE     DogCatcher allows loop-friendly protein-protein ligation.
  JOURNAL   Cell Chem Biol. 2021 Jul 28. pii: S2451-9456(21)00315-9. doi: 
            10.1016/j.chembiol.2021.07.005.
  PUBMED    34324879
REFERENCE   2  (bases 1 to 834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Chem 
            Biol. 2021 Jul 28. pii: S2451-9456(21)00315-9. doi: 
            10.1016/j.chembiol.2021.07.005."
COMMENT     Sequence Label: pET28-sfGFP-SpyTag003 Loop C
FEATURES             Location/Qualifiers
     source          1..834
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             535..582
                     /codon_start=1
                     /product="improved SpyTag variant that reacts with 
                     SpyCatcher003 at a rate approaching the diffusion limit 
                     (Keeble et al., 2019)"
                     /label=SpyTag003
                     /translation="RGVPHIVMVDAYKRYK"
     CDS             811..828
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgcgtaaag gcgaagagct gttcactggt gtcgtcccta ttctggtgga actggatggt
       61 gatgtcaacg gtcataagtt ttccgtgcgt ggcgagggtg aaggtgacgc aactaatggt
      121 aaactgacgc tgaagttcat ctgtactact ggtaaactgc cggtaccttg gccgactctg
      181 gtaacgacgc tgacttatgg tgttcagtgc tttgctcgtt atccggacca tatgaagcag
      241 catgacttct tcaagtccgc catgccggaa ggctatgtgc aggaacgcac gatttccttt
      301 aaggatgacg gcacgtacaa aacgcgtgcg gaagtgaaat ttgaaggcga taccctggta
      361 aaccgcattg agctgaaagg cattgacttt aaagaagacg gcaatatcct gggccataag
      421 ctggaataca attttaacag ccacaatgtt tacatcaccg ccgataaaca aaaaaatggc
      481 attaaagcga attttaaaat tcgccacaac gtggaggatg gcgggggtgg ctcgcgtggt
      541 gttccacata tcgttatggt tgatgcgtac aaacgctata agggaggagg tggctctggc
      601 agcgtgcagc tggctgatca ctaccagcaa aacactccaa tcggtgatgg tcctgttctg
      661 ctgccagaca atcactatct gagcacgcaa agcgttctgt ctaaagatcc gaacgagaaa
      721 cgcgatcata tggttctgct ggagttcgta accgcagcgg gcatcacgca tggtatggat
      781 gaactgtaca aaggctccgg ctccggctcc caccatcacc atcaccattg ataa
//
