LOCUS       sequence-333193-         855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses in bacterial cytoplasm superfolder GFP with DogTag and 
            flanking linkers between Val22 and Asn23.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Keeble AH, Yadav VK, Ferla MP, Bauer CC, Chuntharpursat-Bon E, Huang
            J, Bon RS, Howarth M
  TITLE     DogCatcher allows loop-friendly protein-protein ligation.
  JOURNAL   Cell Chem Biol. 2021 Jul 28. pii: S2451-9456(21)00315-9. doi: 
            10.1016/j.chembiol.2021.07.005.
  PUBMED    34324879
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Chem 
            Biol. 2021 Jul 28. pii: S2451-9456(21)00315-9. doi: 
            10.1016/j.chembiol.2021.07.005."
COMMENT     Sequence Label: pET28-sfGFP-DogTag Loop A
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             82..150
                     /codon_start=1
                     /product="peptide derived from the RrgA adhesin of 
                     Streptococcus pneumoniae"
                     /label=DogTag
                     /note="forms a covalent bond with the DogCatcher protein 
                     when inserted into an intra-domain protein loop (Keeble et 
                     al., 2022)"
                     /translation="DIPATYEFTDGKHYITNEPIPPK"
     CDS             832..849
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgcgtaaag gcgaagagct gttcactggt gtcgtcccta ttctggtgga actggatggt
       61 gatgtcggag gaggtggctc tgatattccg gctacatacg aattcaccga tggtaaacat
      121 tatatcacca atgaaccgat accgccgaaa ggcgggggtg gctcgaacgg tcataagttt
      181 tccgtgcgtg gcgagggtga aggtgacgca actaatggta aactgacgct gaagttcatc
      241 tgtactactg gtaaactgcc ggtaccttgg ccgactctgg taacgacgct gacttatggt
      301 gttcagtgct ttgctcgtta tccggaccat atgaagcagc atgacttctt caagtccgcc
      361 atgccggaag gctatgtgca ggaacgcacg atttccttta aggatgacgg cacgtacaaa
      421 acgcgtgcgg aagtgaaatt tgaaggcgat accctggtaa accgcattga gctgaaaggc
      481 attgacttta aagaagacgg caatatcctg ggccataagc tggaatacaa ttttaacagc
      541 cacaatgttt acatcaccgc cgataaacaa aaaaatggca ttaaagcgaa ttttaaaatt
      601 cgccacaacg tggaggatgg cagcgtgcag ctggctgatc actaccagca aaacactcca
      661 atcggtgatg gtcctgttct gctgccagac aatcactatc tgagcacgca aagcgttctg
      721 tctaaagatc cgaacgagaa acgcgatcat atggttctgc tggagttcgt aaccgcagcg
      781 ggcatcacgc atggtatgga tgaactgtac aaaggctccg gctccggctc ccaccatcac
      841 catcaccatt gataa
//
