LOCUS       sequence-333195-         855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses in bacterial cytoplasm superfolder GFP with DogTag and 
            flanking linkers between Asp173 and Gly174.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Keeble AH, Yadav VK, Ferla MP, Bauer CC, Chuntharpursat-Bon E, Huang
            J, Bon RS, Howarth M
  TITLE     DogCatcher allows loop-friendly protein-protein ligation.
  JOURNAL   Cell Chem Biol. 2021 Jul 28. pii: S2451-9456(21)00315-9. doi: 
            10.1016/j.chembiol.2021.07.005.
  PUBMED    34324879
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Chem 
            Biol. 2021 Jul 28. pii: S2451-9456(21)00315-9. doi: 
            10.1016/j.chembiol.2021.07.005."
COMMENT     Sequence Label: pET28-sfGFP-DogTag Loop C
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             535..603
                     /codon_start=1
                     /product="peptide derived from the RrgA adhesin of 
                     Streptococcus pneumoniae"
                     /label=DogTag
                     /note="forms a covalent bond with the DogCatcher protein 
                     when inserted into an intra-domain protein loop (Keeble et 
                     al., 2022)"
                     /translation="DIPATYEFTDGKHYITNEPIPPK"
     CDS             832..849
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgcgtaaag gcgaagagct gttcactggt gtcgtcccta ttctggtgga actggatggt
       61 gatgtcaacg gtcataagtt ttccgtgcgt ggcgagggtg aaggtgacgc aactaatggt
      121 aaactgacgc tgaagttcat ctgtactact ggtaaactgc cggtaccttg gccgactctg
      181 gtaacgacgc tgacttatgg tgttcagtgc tttgctcgtt atccggacca tatgaagcag
      241 catgacttct tcaagtccgc catgccggaa ggctatgtgc aggaacgcac gatttccttt
      301 aaggatgacg gcacgtacaa aacgcgtgcg gaagtgaaat ttgaaggcga taccctggta
      361 aaccgcattg agctgaaagg cattgacttt aaagaagacg gcaatatcct gggccataag
      421 ctggaataca attttaacag ccacaatgtt tacatcaccg ccgataaaca aaaaaatggc
      481 attaaagcga attttaaaat tcgccacaac gtggaggatg gaggaggtgg ctctgatatt
      541 ccggctacat acgaattcac cgatggtaaa cattatatca ccaatgaacc gataccgccg
      601 aaaggcgggg gtggctcggg cagcgtgcag ctggctgatc actaccagca aaacactcca
      661 atcggtgatg gtcctgttct gctgccagac aatcactatc tgagcacgca aagcgttctg
      721 tctaaagatc cgaacgagaa acgcgatcat atggttctgc tggagttcgt aaccgcagcg
      781 ggcatcacgc atggtatgga tgaactgtac aaaggctccg gctccggctc ccaccatcac
      841 catcaccatt gataa
//
