LOCUS       sequence-333200-       11002 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-terminal aid-V5 degron cassette with kanMX6 marker for S. pombe.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11002)
  TITLE     Carr lab yeast expression plasmids
REFERENCE   2  (bases 1 to 11002)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAW8-aid-V5-Turg1:kanMX6
FEATURES             Location/Qualifiers
     source          1..11002
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             158..1252
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     rep_origin      2084..2865
                     /label=ars1
                     /note="Schizosaccharomyces pombe autonomously replicating 
                     sequence ars1"
     primer_bind     complement(2960..2979)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3079..3101
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3139..3157)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3225..3329
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3330..3398,3399..4190)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3548..3567)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4361..4949
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4850..4869
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5103..5120
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5237..5258
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5273..5278,5279..5296,5297..5303)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5311..5327
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5316..5338
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5335..5351
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5335..5351
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    complement(5383..5416)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             5455..6141
                     /codon_start=1
                     /gene="Arabidopsis thaliana AXR3"
                     /product="auxin-responsive IAA17 protein"
                     /label=auxin-inducible degron
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="MMGSVELNLRETELCLGLPGGDTVAPVTGNKRGFSETVDLKLNLN
                     NEPANKEGSTTHDVVTFDSKEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSS
                     GGPEAAAFVKVSMDGAPYLRKIDLRMYKSYDELSNALSNMFSSFTMGKHGGEEGMIDFM
                     NERKLMDLVNSWDYVPSYEDKDGDWMLVGDVPWPMFVDTCKRLRLMKGSDAIGLAPRAM
                     EKCKSRA"
     CDS             6160..6201
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     gene            6798..8154
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     promoter        6798..7141
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             7142..7951
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(7209..7228)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      7957..8154
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(8249..8266)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8249..8265)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8258..8280)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gcatcacaat acttgaagtt gacaatatta tttaaggacc tattgttttt tccaataggt
       61 ggttagcaat cgtcttactt tctaactttt cttacctttt acatttcagc aatatatata
      121 tatatatata tatatatttc aaggatatac cattgtaatg tctgccccta agaagatcgt
      181 cgttttgcca ggtgaccacg ttggtcaaga aatcacagcc gaagccatta aggttcttaa
      241 agctatttct gatgttcgtt ccaatgtcaa gttcgatttc gaaaatcatt taattggtgg
      301 tgctgctatc gatgctacag gtgttccact tccagatgag gcgctggaag cctccaagaa
      361 ggctgatgcc gttttgttag gtgctgtggg tggtcctaaa tggggtaccg gtagtgttag
      421 acctgaacaa ggtttactaa aaatccgtaa agaacttcaa ttgtacgcca acttaagacc
      481 atgtaacttt gcatccgact ctcttttaga cttatctcca atcaagccac aatttgctaa
      541 aggtactgac ttcgttgttg ttagagaatt agtgggaggt atttactttg gtaagagaaa
      601 ggaagacgat ggtgatggtg tcgcttggga tagtgaacaa tacaccgttc cagaagtgca
      661 aagaatcaca agaatggccg ctttcatggc cctacaacat gagccaccat tgcctatttg
      721 gtccttggat aaagctaatg ttttggcctc ttcaagatta tggagaaaaa ctgtggagga
      781 aaccatcaag aacgaatttc ctacattgaa agttcaacat caattgattg attctgccgc
      841 catgatccta gttaagaacc caacccacct aaatggtatt ataatcacca gcaacatgtt
      901 tggtgatatc atctccgatg aagcctccgt tatcccaggc tccttgggtt tgttgccatc
      961 tgcgtccttg gcctctttgc cagacaagaa caccgcattt ggtttgtacg aaccatgcca
     1021 tggttccgct ccagatttgc caaagaataa ggtcaaccct atcgccacta tcttgtctgc
     1081 tgcaatgatg ttgaaattgt cattgaactt gcctgaagaa ggtaaagcca ttgaagatgc
     1141 agttaaaaag gttttggatg caggtatcag aactggtgat ttaggtggtt ccaacagtac
     1201 caccgaagtc ggtgatgctg tcgccgaaga agttaagaaa atccttgctt aaaaagattc
     1261 tctttttttg tgatatttgt acataaactt tataaatgaa attcataata gaaacgacac
     1321 gaaattacaa aatggaatat gttcataggg tagacgaaac tatatacgca atctacatac
     1381 atttatcaag aaggagaaaa aggaggatgt aaaggaatac aggtaagcaa attgatacta
     1441 atggctcaac gtgataagga aaaagaattg cactttaaca ttaatattga caaggaggag
     1501 ggcatcacac aaaaagttag gtgtaacaga aaatcatgaa actatgattt ctaatttata
     1561 tattggagga ttttctctaa aaaaaaaaat acaacaaata aaaaacactc aatgacctga
     1621 ccatttgatg gagtttaagt caataccttc ttgaaccatt tcccataatg gtgaaagttc
     1681 cctcaagaat tttactctgt cagaaacggc cttaacgacg tagtcgatcc ggacgatggc
     1741 caaccttcca attcattaaa tcgagtcctc catcagatga tttacaaggt tttgcataga
     1801 atccctaagt aagagaagtt gttagtgatt tcttcaagtt tactaaaatt tacatacaaa
     1861 tggatgatct cttctccatt gtttcctttc ttcttgcaaa cgcgttttac aaagagatga
     1921 catgctattt tctgattatt tttttctata gttttctatt ttgtacgtcc ttgggagtcc
     1981 gaaatgtaaa atcggggtat ggttagtagc ggcagcgttc attatggtat ataaaagata
     2041 aaaagtaggg gcaagcgaga cagaatggga tacaagggca tcgtctataa ttatagctaa
     2101 aaattgtatt ttaatttgta ttttttgtaa ttttatttct cattgtttta cttaaaatga
     2161 atgcgaatta gagaaaactt caacgaaatg tcaaaataag ctcagcaaaa tatacaattt
     2221 tagggaaagc gatcagcaac ttattctccg ttgtagattc tttttcttca taaattccaa
     2281 caatgtaact gccaattgat ttttctctag atgctaataa attgaaacta atgaatataa
     2341 taacacaggt tttgataaat tgtaaactct tactcaaaaa aagaatgtta aaactaattt
     2401 aaccttttta taaaggctca tggcgttaaa gaggatagta aaaattctac aattttttaa
     2461 tttaatgtca caattgtctt gcgaatataa ttctattaat tttcgctaac tgctgtttta
     2521 gtactattac tttcaatatg ccttatatgc aactttaatt catgaagaat gcaatatttt
     2581 tgcaatcttt cttgtactga gcgcttttta tcatttagta ctcattattt aatttttcag
     2641 taaagggcaa taaataaatt tgtagaagat gcaatgtaat ctcctctatc cttttgctca
     2701 tatgtttatg agtataccta gtctagaaag gcttgtattt aaaatattat tcaataaaaa
     2761 ttcacaattt ttacgacatg tgctaccatt cacttaactt cctgattata aaattggttc
     2821 gtttatacta attacttaag tacctttaac taaacaaaat gcctatatat atattaattt
     2881 acaatgagtg tcagataagt cactatgtcc gagtggttaa ggagttagac tcgccatcga
     2941 tggtatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac
     3001 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     3061 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     3121 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     3181 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     3241 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     3301 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     3361 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     3421 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     3481 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     3541 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     3601 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     3661 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     3721 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     3781 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     3841 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     3901 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     3961 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     4021 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     4081 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     4141 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     4201 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     4261 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     4321 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     4381 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     4441 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     4501 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     4561 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     4621 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     4681 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     4741 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     4801 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     4861 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     4921 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     4981 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     5041 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     5101 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     5161 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     5221 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     5281 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     5341 acagctatga ccatgattac gccaagcttg catggcggcc gcataacttc gtatagcata
     5401 cattatacga agttatgcat gctcggtggg tcaggtggaa gtggatctgg tgctatgatg
     5461 ggcagtgtcg agctgaatct gagggagact gagctgtgtc ttggtcttcc cggtggagat
     5521 acagtggctc cggtaaccgg aaacaagaga gggttctcag agacggttga tctgaagcta
     5581 aatctgaata atgagcctgc aaacaaggaa ggatctacga ctcatgacgt cgtgactttt
     5641 gattccaagg agaagagtgc ttgtcctaaa gatccagcca aacctccggc caaggcacaa
     5701 gttgtgggat ggccaccggt gagatcatac cggaagaacg tgatggtttc ctgccaaaaa
     5761 tcaagcggtg gcccggaggc ggcggcgttc gtgaaggtat caatggacgg agcaccgtac
     5821 ttgaggaaaa tcgatttgag gatgtataaa agctacgatg agctttctaa tgctttgtcc
     5881 aacatgttca gctcttttac catgggcaaa catggaggag aagaaggaat gatagacttc
     5941 atgaatgaga ggaaattgat ggatttggtg aatagctggg actatgttcc ctcttatgaa
     6001 gacaaagacg gtgattggat gctcgtcggc gacgttcctt ggccaatgtt cgtcgataca
     6061 tgcaagcgtt tacgtctcat gaaaggatcg gatgccattg gtctcgctcc gagggcgatg
     6121 gagaagtgca agagcagagc tggtgctgga gcaggcgccg gcaaaccgat ccctaaccca
     6181 ctcctgggcc tggactcaac atgagctagc agtcccgggg cttgcccatc tgttttagac
     6241 gtcacagaag gaccatgttt attttaaatt tcacatacat gtgtttaatt accttcattg
     6301 tataaattct cgaaactttc tccaattctt cggtcagata aaaaatttct ctagtcaccg
     6361 tcttttatag agcttttgtc agatgttttt ctggatggaa tcattgtcca gttactatgc
     6421 tttttattta taagtttgaa agtttagtta actttttgtt ttctttaata cattttttaa
     6481 tcatgtcaac atttgggatt tgtagaagta gtaaattttt tgtacatgtt gtagaattgg
     6541 ataagaacga aaacagctag tatgtaaaaa aaacgaaaat cccaaattcc gttgtttgtt
     6601 tgatatagta atatttatag cattttagtt tctaaagatt tttgataatc ttttacattt
     6661 gctttcacat acattcgcgc tactaccata catcatatcc cgctttcttt aattgcttcc
     6721 ccacagactc aaccagcggg gccttcttct cgagatctgt ttagcttgcc tcgtccccgc
     6781 cgggtcaccc ggccagcgac atggaggccc agaataccct ccttgacagt cttgacgtgc
     6841 gcagctcagg ggcatgatgt gactgtcgcc cgtacattta gcccatacat ccccatgtat
     6901 aatcatttgc atccatacat tttgatggcc gcacggcgcg aagcaaaaat tacggctcct
     6961 cgctgcagac ctgcgagcag ggaaacgctc ccctcacaga cgcgttgaat tgtccccacg
     7021 ccgcgcccct gtagagaaat ataaaaggtt aggatttgcc actgaggttc ttctttcata
     7081 tacttccttt taaaatcttg ctaggataca gttctcacat cacatccgaa cataaacaac
     7141 catgggtaag gaaaagactc acgtttcgag gccgcgatta aattccaaca tggatgctga
     7201 tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg
     7261 attgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc
     7321 caatgatgtt acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc
     7381 gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc
     7441 cggcaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga
     7501 tgcgctggca gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa
     7561 cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga
     7621 tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat
     7681 gcataagctt ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga
     7741 taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat
     7801 cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc
     7861 attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca
     7921 gtttcatttg atgctcgatg agtttttcta atcagtactg acaataaaaa gattcttgtt
     7981 ttcaagaact tgtcatttgt atagtttttt tatattgtag ttgttctatt ttaatcaaat
     8041 gttagcgtga tttatatttt ttttcgcctc gacatcatct gcccagatgc gaagttaagt
     8101 gcgcagaaag taatatcatg cgtcaatcgt atgtgaatgc tggtcgctat actgctgtcg
     8161 attcgatact aacgccgcca tccagtcgac gaactagtat aacttcgtat atggtattat
     8221 atacgaagtt atgcggccgc gcgaattcac tggccgtcgt tttacaacgt cgtgactggg
     8281 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
     8341 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     8401 aatggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatac
     8461 catcgtccgg atcaatgaag tagatgagtt tactacctgt atatactttt ttttttgtca
     8521 aagtcacttt tatggcattt ccatgttgaa ggattagcca cagcactaaa ttttcagcgt
     8581 atgattgctt ttaaatattt aattttcatc gttttttaat taatattcaa aacgatttaa
     8641 tgccttctac atctgaagtc aagctcatag actcgggttg ggtagctaaa actacatcta
     8701 acacgaaggg gtatagcctt cttaaagtat actcatgtat aaacgttttg ggttcatatt
     8761 tttgggccac gtggtttata aaaattctta actacaccac tcggtatccc gcacccgtct
     8821 acgtttctac gatttcgagc taatattgag taaagtgatt agcaaaaaaa ataaaagtac
     8881 tcgttgtcgg agatcaagaa tttttctatt atctcatcta aaccactttc taaaagcgaa
     8941 aaacaaaatc gtaatatgca gcttgaatgg gcttccatag tttgaaagaa aaaccctagc
     9001 agtactggca agggagacat tccttttacc tggctaatcg ccatcttcca gcaggcgcac
     9061 cattgcccct gtttcactat ccaggttacg gatatagttc atgacaatat ttacattggt
     9121 ccagccacca gcttgcatga tctccggtat tgaaactcca gcgcgggcca tatctcgcgc
     9181 ggctccgaca cgggcactgt gtccagacca ggccaggtat ctctgaccag agtcatcctt
     9241 agcgccgtaa atcaatcgat gagttgcttc aaaaatccct tccagggcgc gagttgatag
     9301 ctggctggtg gcagatggcg cggcaacacc attttttctg acccggcaaa acaggtagtt
     9361 attcggatca tcagctacac cagagacgga aatccatcgc tcgaccagtt tagttacccc
     9421 caggctaagt gccttctcta cacctgcggt gctaaccagc gttttcgttc tgccaatatg
     9481 gattaacatt ctcccaccgt cagtacgtga gatatcttta accctgatcc tggcaatttc
     9541 ggctatacgt aacagggtgt tataagcaat ccccagaaat gccagattac gtatatcctg
     9601 gcagcgatcg ctattttcca tgagtgaacg aacctggtcg aaatcagtgc gttcgaacgc
     9661 tagagcctgt tttgcacgtt caccggcatc aacgttttct tttcggatcc gccgcataac
     9721 cagtgaaaca gcattgctgt cacttggtcg tggcagcccg gaccgacgat gaagcatgtt
     9781 tagctggccc aaatgttgct ggatagtttt tactgccaga ccgcgcgcct gaagatatag
     9841 aagataatcg cgaacatctt caggttctgc gggaaaccat ttccggttat tcaacttgca
     9901 ccatgccgcc cacgaccggc aaacggacag aagcattttc caggtatgct cagaaaacgc
     9961 ctggcgatcc ctgaacatgt ccatcaggtt cttgcgaacc tcatcactcg ttgcatcgac
    10021 cggtaacttt tagattaaca tttgtttaaa tatgaaatta ggcaatgtaa catactgcag
    10081 gcaaattttg gtgtacggta agtaaattgg acatatgatt taacaaagcg actataagtc
    10141 agaaagtgag aatgagattg aaataattaa ttcaacttat tcaattgatg atatgccagg
    10201 attcctcttc ctttatcttc aatgtttcga ttaacaggtc cattatccgg tttttagttt
    10261 cgctgcacat tgccgaatga caatcggatt cctttaagct ttgcttttgc cctgttttct
    10321 ctccccagta atcgttgggt gactgaacca tccagtagcc gagagttacg tttttcctga
    10381 tttaataaat atccatatct atttgtctcg tggtgtttta cagcaactaa ccgaaattac
    10441 tattttgcat catccaacca taaagagaca acaataattc tagaaaacat ataacaagtg
    10501 tttttttcta tttacgtctc atacgcaaag tccactttac catgttttgg aaacttgtac
    10561 ccaaccattc atttttcgat tttgtaatga ggctcttttc ttctaacaat cattgaggtc
    10621 attatcatgt gtcctcacct attgtttaaa ttgaggcttg cgctattccc aaaattgtgg
    10681 gtattatttt catatatcag tatctgcaat ttcggttcaa ctccggaagc tcggtaccca
    10741 actgtgggaa tactcaggta tcgtaagatg caagagttcg aatctcttag caaccattat
    10801 tttttttctc tcaacataac gagaacacac aggggcgcta tcgcacagaa tcaaattcga
    10861 tgactggaaa ttttttgtta atttcagagg tcgcctgacg catatacctt tttcaactga
    10921 aaaattggga gaaaaaggaa aggtgagagc gccggaaccg gcttttcata tagaatagag
    10981 aagcgttcat gactaaatgc tt
//
