LOCUS       sequence-333201-       11156 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-terminal aid-V5-spo5DSR degron cassette with kanMX6 marker for S. 
            pombe.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11156)
  TITLE     Carr lab yeast expression plasmids
REFERENCE   2  (bases 1 to 11156)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAW8-aid-V5-spo5DSR-Turg1:kanMX6
FEATURES             Location/Qualifiers
     source          1..11156
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             188..1282
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     rep_origin      2114..2895
                     /label=ars1
                     /note="Schizosaccharomyces pombe autonomously replicating 
                     sequence ars1"
     primer_bind     complement(2990..3009)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3109..3131
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3169..3187)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3255..3359
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3360..3428,3429..4220)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3578..3597)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4391..4979
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4880..4899
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5133..5150
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5267..5288
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5303..5308,5309..5326,5327..5333)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5341..5357
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5346..5368
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5365..5381
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5365..5381
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    complement(5413..5446)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             5485..6171
                     /codon_start=1
                     /gene="Arabidopsis thaliana AXR3"
                     /product="auxin-responsive IAA17 protein"
                     /label=auxin-inducible degron
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="MMGSVELNLRETELCLGLPGGDTVAPVTGNKRGFSETVDLKLNLN
                     NEPANKEGSTTHDVVTFDSKEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSS
                     GGPEAAAFVKVSMDGAPYLRKIDLRMYKSYDELSNALSNMFSSFTMGKHGGEEGMIDFM
                     NERKLMDLVNSWDYVPSYEDKDGDWMLVGDVPWPMFVDTCKRLRLMKGSDAIGLAPRAM
                     EKCKSRA"
     CDS             6190..6231
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     gene            6982..8338
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     promoter        6982..7325
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             7326..8135
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(7393..7412)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      8141..8338
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(8433..8450)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8433..8449)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8442..8464)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gaatagagaa gcgttcatga ctaaatgctt gcatcacaat acttgaagtt gacaatatta
       61 tttaaggacc tattgttttt tccaataggt ggttagcaat cgtcttactt tctaactttt
      121 cttacctttt acatttcagc aatatatata tatatatata tatatatttc aaggatatac
      181 cattgtaatg tctgccccta agaagatcgt cgttttgcca ggtgaccacg ttggtcaaga
      241 aatcacagcc gaagccatta aggttcttaa agctatttct gatgttcgtt ccaatgtcaa
      301 gttcgatttc gaaaatcatt taattggtgg tgctgctatc gatgctacag gtgttccact
      361 tccagatgag gcgctggaag cctccaagaa ggctgatgcc gttttgttag gtgctgtggg
      421 tggtcctaaa tggggtaccg gtagtgttag acctgaacaa ggtttactaa aaatccgtaa
      481 agaacttcaa ttgtacgcca acttaagacc atgtaacttt gcatccgact ctcttttaga
      541 cttatctcca atcaagccac aatttgctaa aggtactgac ttcgttgttg ttagagaatt
      601 agtgggaggt atttactttg gtaagagaaa ggaagacgat ggtgatggtg tcgcttggga
      661 tagtgaacaa tacaccgttc cagaagtgca aagaatcaca agaatggccg ctttcatggc
      721 cctacaacat gagccaccat tgcctatttg gtccttggat aaagctaatg ttttggcctc
      781 ttcaagatta tggagaaaaa ctgtggagga aaccatcaag aacgaatttc ctacattgaa
      841 agttcaacat caattgattg attctgccgc catgatccta gttaagaacc caacccacct
      901 aaatggtatt ataatcacca gcaacatgtt tggtgatatc atctccgatg aagcctccgt
      961 tatcccaggc tccttgggtt tgttgccatc tgcgtccttg gcctctttgc cagacaagaa
     1021 caccgcattt ggtttgtacg aaccatgcca tggttccgct ccagatttgc caaagaataa
     1081 ggtcaaccct atcgccacta tcttgtctgc tgcaatgatg ttgaaattgt cattgaactt
     1141 gcctgaagaa ggtaaagcca ttgaagatgc agttaaaaag gttttggatg caggtatcag
     1201 aactggtgat ttaggtggtt ccaacagtac caccgaagtc ggtgatgctg tcgccgaaga
     1261 agttaagaaa atccttgctt aaaaagattc tctttttttg tgatatttgt acataaactt
     1321 tataaatgaa attcataata gaaacgacac gaaattacaa aatggaatat gttcataggg
     1381 tagacgaaac tatatacgca atctacatac atttatcaag aaggagaaaa aggaggatgt
     1441 aaaggaatac aggtaagcaa attgatacta atggctcaac gtgataagga aaaagaattg
     1501 cactttaaca ttaatattga caaggaggag ggcatcacac aaaaagttag gtgtaacaga
     1561 aaatcatgaa actatgattt ctaatttata tattggagga ttttctctaa aaaaaaaaat
     1621 acaacaaata aaaaacactc aatgacctga ccatttgatg gagtttaagt caataccttc
     1681 ttgaaccatt tcccataatg gtgaaagttc cctcaagaat tttactctgt cagaaacggc
     1741 cttaacgacg tagtcgatcc ggacgatggc caaccttcca attcattaaa tcgagtcctc
     1801 catcagatga tttacaaggt tttgcataga atccctaagt aagagaagtt gttagtgatt
     1861 tcttcaagtt tactaaaatt tacatacaaa tggatgatct cttctccatt gtttcctttc
     1921 ttcttgcaaa cgcgttttac aaagagatga catgctattt tctgattatt tttttctata
     1981 gttttctatt ttgtacgtcc ttgggagtcc gaaatgtaaa atcggggtat ggttagtagc
     2041 ggcagcgttc attatggtat ataaaagata aaaagtaggg gcaagcgaga cagaatggga
     2101 tacaagggca tcgtctataa ttatagctaa aaattgtatt ttaatttgta ttttttgtaa
     2161 ttttatttct cattgtttta cttaaaatga atgcgaatta gagaaaactt caacgaaatg
     2221 tcaaaataag ctcagcaaaa tatacaattt tagggaaagc gatcagcaac ttattctccg
     2281 ttgtagattc tttttcttca taaattccaa caatgtaact gccaattgat ttttctctag
     2341 atgctaataa attgaaacta atgaatataa taacacaggt tttgataaat tgtaaactct
     2401 tactcaaaaa aagaatgtta aaactaattt aaccttttta taaaggctca tggcgttaaa
     2461 gaggatagta aaaattctac aattttttaa tttaatgtca caattgtctt gcgaatataa
     2521 ttctattaat tttcgctaac tgctgtttta gtactattac tttcaatatg ccttatatgc
     2581 aactttaatt catgaagaat gcaatatttt tgcaatcttt cttgtactga gcgcttttta
     2641 tcatttagta ctcattattt aatttttcag taaagggcaa taaataaatt tgtagaagat
     2701 gcaatgtaat ctcctctatc cttttgctca tatgtttatg agtataccta gtctagaaag
     2761 gcttgtattt aaaatattat tcaataaaaa ttcacaattt ttacgacatg tgctaccatt
     2821 cacttaactt cctgattata aaattggttc gtttatacta attacttaag tacctttaac
     2881 taaacaaaat gcctatatat atattaattt acaatgagtg tcagataagt cactatgtcc
     2941 gagtggttaa ggagttagac tcgccatcga tggtatggtg cactctcagt acaatctgct
     3001 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
     3061 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     3121 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac
     3181 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     3241 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     3301 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     3361 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     3421 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     3481 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     3541 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     3601 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     3661 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     3721 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     3781 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     3841 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     3901 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     3961 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     4021 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     4081 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     4141 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     4201 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     4261 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     4321 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     4381 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     4441 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     4501 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     4561 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     4621 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     4681 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     4741 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     4801 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     4861 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     4921 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     4981 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     5041 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     5101 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     5161 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     5221 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     5281 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     5341 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg
     5401 catggcggcc gcataacttc gtatagcata cattatacga agttatgcat gctcggtggg
     5461 tcaggtggaa gtggatctgg tgctatgatg ggcagtgtcg agctgaatct gagggagact
     5521 gagctgtgtc ttggtcttcc cggtggagat acagtggctc cggtaaccgg aaacaagaga
     5581 gggttctcag agacggttga tctgaagcta aatctgaata atgagcctgc aaacaaggaa
     5641 ggatctacga ctcatgacgt cgtgactttt gattccaagg agaagagtgc ttgtcctaaa
     5701 gatccagcca aacctccggc caaggcacaa gttgtgggat ggccaccggt gagatcatac
     5761 cggaagaacg tgatggtttc ctgccaaaaa tcaagcggtg gcccggaggc ggcggcgttc
     5821 gtgaaggtat caatggacgg agcaccgtac ttgaggaaaa tcgatttgag gatgtataaa
     5881 agctacgatg agctttctaa tgctttgtcc aacatgttca gctcttttac catgggcaaa
     5941 catggaggag aagaaggaat gatagacttc atgaatgaga ggaaattgat ggatttggtg
     6001 aatagctggg actatgttcc ctcttatgaa gacaaagacg gtgattggat gctcgtcggc
     6061 gacgttcctt ggccaatgtt cgtcgataca tgcaagcgtt tacgtctcat gaaaggatcg
     6121 gatgccattg gtctcgctcc gagggcgatg gagaagtgca agagcagagc tggtgctgga
     6181 gcaggcgccg gcaaaccgat ccctaaccca ctcctgggcc tggactcaac atgagctagc
     6241 actacgccat atcatgccca tcccgctact tctgctgcta attaaattta aacatctttc
     6301 cagcatttac ttgaacatca aactctaata tcgaaatctt ttcaaacact tatcacatac
     6361 tcaaacttaa accaacataa aacctgttag acaaagcccc ggggcttgcc catctgtttt
     6421 agacgtcaca gaaggaccat gtttatttta aatttcacat acatgtgttt aattaccttc
     6481 attgtataaa ttctcgaaac tttctccaat tcttcggtca gataaaaaat ttctctagtc
     6541 accgtctttt atagagcttt tgtcagatgt ttttctggat ggaatcattg tccagttact
     6601 atgcttttta tttataagtt tgaaagttta gttaactttt tgttttcttt aatacatttt
     6661 ttaatcatgt caacatttgg gatttgtaga agtagtaaat tttttgtaca tgttgtagaa
     6721 ttggataaga acgaaaacag ctagtatgta aaaaaaacga aaatcccaaa ttccgttgtt
     6781 tgtttgatat agtaatattt atagcatttt agtttctaaa gatttttgat aatcttttac
     6841 atttgctttc acatacattc gcgctactac catacatcat atcccgcttt ctttaattgc
     6901 ttccccacag actcaaccag cggggccttc ttctcgagat ctgtttagct tgcctcgtcc
     6961 ccgccgggtc acccggccag cgacatggag gcccagaata ccctccttga cagtcttgac
     7021 gtgcgcagct caggggcatg atgtgactgt cgcccgtaca tttagcccat acatccccat
     7081 gtataatcat ttgcatccat acattttgat ggccgcacgg cgcgaagcaa aaattacggc
     7141 tcctcgctgc agacctgcga gcagggaaac gctcccctca cagacgcgtt gaattgtccc
     7201 cacgccgcgc ccctgtagag aaatataaaa ggttaggatt tgccactgag gttcttcttt
     7261 catatacttc cttttaaaat cttgctagga tacagttctc acatcacatc cgaacataaa
     7321 caaccatggg taaggaaaag actcacgttt cgaggccgcg attaaattcc aacatggatg
     7381 ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct
     7441 atcgattgta tgggaagccc gatgcgccag agttgtttct gaaacatggc aaaggtagcg
     7501 ttgccaatga tgttacagat gagatggtca gactaaactg gctgacggaa tttatgcctc
     7561 ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc accactgcga
     7621 tccccggcaa aacagcattc caggtattag aagaatatcc tgattcaggt gaaaatattg
     7681 ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt
     7741 ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg
     7801 ttgatgcgag tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag
     7861 aaatgcataa gcttttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac
     7921 ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt ggacgagtcg
     7981 gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt gagttttctc
     8041 cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat
     8101 tgcagtttca tttgatgctc gatgagtttt tctaatcagt actgacaata aaaagattct
     8161 tgttttcaag aacttgtcat ttgtatagtt tttttatatt gtagttgttc tattttaatc
     8221 aaatgttagc gtgatttata ttttttttcg cctcgacatc atctgcccag atgcgaagtt
     8281 aagtgcgcag aaagtaatat catgcgtcaa tcgtatgtga atgctggtcg ctatactgct
     8341 gtcgattcga tactaacgcc gccatccagt cgacgaacta gtataacttc gtatatggta
     8401 ttatatacga agttatgcgg ccgcgcgaat tcactggccg tcgttttaca acgtcgtgac
     8461 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     8521 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     8581 ggcgaatggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
     8641 ataccatcgt ccggatcaat gaagtagatg agtttactac ctgtatatac tttttttttt
     8701 gtcaaagtca cttttatggc atttccatgt tgaaggatta gccacagcac taaattttca
     8761 gcgtatgatt gcttttaaat atttaatttt catcgttttt taattaatat tcaaaacgat
     8821 ttaatgcctt ctacatctga agtcaagctc atagactcgg gttgggtagc taaaactaca
     8881 tctaacacga aggggtatag ccttcttaaa gtatactcat gtataaacgt tttgggttca
     8941 tatttttggg ccacgtggtt tataaaaatt cttaactaca ccactcggta tcccgcaccc
     9001 gtctacgttt ctacgatttc gagctaatat tgagtaaagt gattagcaaa aaaaataaaa
     9061 gtactcgttg tcggagatca agaatttttc tattatctca tctaaaccac tttctaaaag
     9121 cgaaaaacaa aatcgtaata tgcagcttga atgggcttcc atagtttgaa agaaaaaccc
     9181 tagcagtact ggcaagggag acattccttt tacctggcta atcgccatct tccagcaggc
     9241 gcaccattgc ccctgtttca ctatccaggt tacggatata gttcatgaca atatttacat
     9301 tggtccagcc accagcttgc atgatctccg gtattgaaac tccagcgcgg gccatatctc
     9361 gcgcggctcc gacacgggca ctgtgtccag accaggccag gtatctctga ccagagtcat
     9421 ccttagcgcc gtaaatcaat cgatgagttg cttcaaaaat cccttccagg gcgcgagttg
     9481 atagctggct ggtggcagat ggcgcggcaa caccattttt tctgacccgg caaaacaggt
     9541 agttattcgg atcatcagct acaccagaga cggaaatcca tcgctcgacc agtttagtta
     9601 cccccaggct aagtgccttc tctacacctg cggtgctaac cagcgttttc gttctgccaa
     9661 tatggattaa cattctccca ccgtcagtac gtgagatatc tttaaccctg atcctggcaa
     9721 tttcggctat acgtaacagg gtgttataag caatccccag aaatgccaga ttacgtatat
     9781 cctggcagcg atcgctattt tccatgagtg aacgaacctg gtcgaaatca gtgcgttcga
     9841 acgctagagc ctgttttgca cgttcaccgg catcaacgtt ttcttttcgg atccgccgca
     9901 taaccagtga aacagcattg ctgtcacttg gtcgtggcag cccggaccga cgatgaagca
     9961 tgtttagctg gcccaaatgt tgctggatag tttttactgc cagaccgcgc gcctgaagat
    10021 atagaagata atcgcgaaca tcttcaggtt ctgcgggaaa ccatttccgg ttattcaact
    10081 tgcaccatgc cgcccacgac cggcaaacgg acagaagcat tttccaggta tgctcagaaa
    10141 acgcctggcg atccctgaac atgtccatca ggttcttgcg aacctcatca ctcgttgcat
    10201 cgaccggtaa cttttagatt aacatttgtt taaatatgaa attaggcaat gtaacatact
    10261 gcaggcaaat tttggtgtac ggtaagtaaa ttggacatat gatttaacaa agcgactata
    10321 agtcagaaag tgagaatgag attgaaataa ttaattcaac ttattcaatt gatgatatgc
    10381 caggattcct cttcctttat cttcaatgtt tcgattaaca ggtccattat ccggttttta
    10441 gtttcgctgc acattgccga atgacaatcg gattccttta agctttgctt ttgccctgtt
    10501 ttctctcccc agtaatcgtt gggtgactga accatccagt agccgagagt tacgtttttc
    10561 ctgatttaat aaatatccat atctatttgt ctcgtggtgt tttacagcaa ctaaccgaaa
    10621 ttactatttt gcatcatcca accataaaga gacaacaata attctagaaa acatataaca
    10681 agtgtttttt tctatttacg tctcatacgc aaagtccact ttaccatgtt ttggaaactt
    10741 gtacccaacc attcattttt cgattttgta atgaggctct tttcttctaa caatcattga
    10801 ggtcattatc atgtgtcctc acctattgtt taaattgagg cttgcgctat tcccaaaatt
    10861 gtgggtatta ttttcatata tcagtatctg caatttcggt tcaactccgg aagctcggta
    10921 cccaactgtg ggaatactca ggtatcgtaa gatgcaagag ttcgaatctc ttagcaacca
    10981 ttattttttt tctctcaaca taacgagaac acacaggggc gctatcgcac agaatcaaat
    11041 tcgatgactg gaaatttttt gttaatttca gaggtcgcct gacgcatata cctttttcaa
    11101 ctgaaaaatt gggagaaaaa ggaaaggtga gagcgccgga accggctttt catata
//
