LOCUS       sequence-333202-       10942 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-terminal aid degron cassette with kanMX6 marker for S. pombe.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10942)
  TITLE     Carr lab yeast expression plasmids
REFERENCE   2  (bases 1 to 10942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAW8-aid-Turg1:kanMX6
FEATURES             Location/Qualifiers
     source          1..10942
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1877..2658
                     /label=ars1
                     /note="Schizosaccharomyces pombe autonomously replicating 
                     sequence ars1"
     primer_bind     complement(2753..2772)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2872..2894
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2932..2950)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3018..3122
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3123..3191,3192..3983)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3341..3360)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4154..4742
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4643..4662
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4896..4913
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5030..5051
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5066..5071,5072..5089,5090..5096)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5104..5120
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5109..5131
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5128..5144
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5128..5144
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    complement(5176..5209)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             5248..5937
                     /codon_start=1
                     /gene="Arabidopsis thaliana AXR3"
                     /product="auxin-responsive IAA17 protein"
                     /label=auxin-inducible degron
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="MMGSVELNLRETELCLGLPGGDTVAPVTGNKRGFSETVDLKLNLN
                     NEPANKEGSTTHDVVTFDSKEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSS
                     GGPEAAAFVKVSMDGAPYLRKIDLRMYKSYDELSNALSNMFSSFTMGKHGGEEGMIDFM
                     NERKLMDLVNSWDYVPSYEDKDGDWMLVGDVPWPMFVDTCKRLRLMKGSDAIGLAPRAM
                     EKCKSRA"
     gene            6531..7887
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     promoter        6531..6874
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             6875..7684
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(6942..6961)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      7690..7887
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(7982..7999)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7982..7998)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7991..8013)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             join(10893..10942,1..1045)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
ORIGIN
        1 agaaatcaca gccgaagcca ttaaggttct taaagctatt tctgatgttc gttccaatgt
       61 caagttcgat ttcgaaaatc atttaattgg tggtgctgct atcgatgcta caggtgttcc
      121 acttccagat gaggcgctgg aagcctccaa gaaggctgat gccgttttgt taggtgctgt
      181 gggtggtcct aaatggggta ccggtagtgt tagacctgaa caaggtttac taaaaatccg
      241 taaagaactt caattgtacg ccaacttaag accatgtaac tttgcatccg actctctttt
      301 agacttatct ccaatcaagc cacaatttgc taaaggtact gacttcgttg ttgttagaga
      361 attagtggga ggtatttact ttggtaagag aaaggaagac gatggtgatg gtgtcgcttg
      421 ggatagtgaa caatacaccg ttccagaagt gcaaagaatc acaagaatgg ccgctttcat
      481 ggccctacaa catgagccac cattgcctat ttggtccttg gataaagcta atgttttggc
      541 ctcttcaaga ttatggagaa aaactgtgga ggaaaccatc aagaacgaat ttcctacatt
      601 gaaagttcaa catcaattga ttgattctgc cgccatgatc ctagttaaga acccaaccca
      661 cctaaatggt attataatca ccagcaacat gtttggtgat atcatctccg atgaagcctc
      721 cgttatccca ggctccttgg gtttgttgcc atctgcgtcc ttggcctctt tgccagacaa
      781 gaacaccgca tttggtttgt acgaaccatg ccatggttcc gctccagatt tgccaaagaa
      841 taaggtcaac cctatcgcca ctatcttgtc tgctgcaatg atgttgaaat tgtcattgaa
      901 cttgcctgaa gaaggtaaag ccattgaaga tgcagttaaa aaggttttgg atgcaggtat
      961 cagaactggt gatttaggtg gttccaacag taccaccgaa gtcggtgatg ctgtcgccga
     1021 agaagttaag aaaatccttg cttaaaaaga ttctcttttt ttgtgatatt tgtacataaa
     1081 ctttataaat gaaattcata atagaaacga cacgaaatta caaaatggaa tatgttcata
     1141 gggtagacga aactatatac gcaatctaca tacatttatc aagaaggaga aaaaggagga
     1201 tgtaaaggaa tacaggtaag caaattgata ctaatggctc aacgtgataa ggaaaaagaa
     1261 ttgcacttta acattaatat tgacaaggag gagggcatca cacaaaaagt taggtgtaac
     1321 agaaaatcat gaaactatga tttctaattt atatattgga ggattttctc taaaaaaaaa
     1381 aatacaacaa ataaaaaaca ctcaatgacc tgaccatttg atggagttta agtcaatacc
     1441 ttcttgaacc atttcccata atggtgaaag ttccctcaag aattttactc tgtcagaaac
     1501 ggccttaacg acgtagtcga tccggacgat ggccaacctt ccaattcatt aaatcgagtc
     1561 ctccatcaga tgatttacaa ggttttgcat agaatcccta agtaagagaa gttgttagtg
     1621 atttcttcaa gtttactaaa atttacatac aaatggatga tctcttctcc attgtttcct
     1681 ttcttcttgc aaacgcgttt tacaaagaga tgacatgcta ttttctgatt atttttttct
     1741 atagttttct attttgtacg tccttgggag tccgaaatgt aaaatcgggg tatggttagt
     1801 agcggcagcg ttcattatgg tatataaaag ataaaaagta ggggcaagcg agacagaatg
     1861 ggatacaagg gcatcgtcta taattatagc taaaaattgt attttaattt gtattttttg
     1921 taattttatt tctcattgtt ttacttaaaa tgaatgcgaa ttagagaaaa cttcaacgaa
     1981 atgtcaaaat aagctcagca aaatatacaa ttttagggaa agcgatcagc aacttattct
     2041 ccgttgtaga ttctttttct tcataaattc caacaatgta actgccaatt gatttttctc
     2101 tagatgctaa taaattgaaa ctaatgaata taataacaca ggttttgata aattgtaaac
     2161 tcttactcaa aaaaagaatg ttaaaactaa tttaaccttt ttataaaggc tcatggcgtt
     2221 aaagaggata gtaaaaattc tacaattttt taatttaatg tcacaattgt cttgcgaata
     2281 taattctatt aattttcgct aactgctgtt ttagtactat tactttcaat atgccttata
     2341 tgcaacttta attcatgaag aatgcaatat ttttgcaatc tttcttgtac tgagcgcttt
     2401 ttatcattta gtactcatta tttaattttt cagtaaaggg caataaataa atttgtagaa
     2461 gatgcaatgt aatctcctct atccttttgc tcatatgttt atgagtatac ctagtctaga
     2521 aaggcttgta tttaaaatat tattcaataa aaattcacaa tttttacgac atgtgctacc
     2581 attcacttaa cttcctgatt ataaaattgg ttcgtttata ctaattactt aagtaccttt
     2641 aactaaacaa aatgcctata tatatattaa tttacaatga gtgtcagata agtcactatg
     2701 tccgagtggt taaggagtta gactcgccat cgatggtatg gtgcactctc agtacaatct
     2761 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct
     2821 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     2881 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga
     2941 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     3001 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     3061 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     3121 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     3181 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     3241 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     3301 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     3361 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     3421 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     3481 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     3541 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     3601 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     3661 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     3721 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     3781 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     3841 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     3901 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     3961 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     4021 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     4081 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     4141 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     4201 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     4261 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     4321 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     4381 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     4441 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     4501 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     4561 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     4621 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     4681 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     4741 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     4801 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     4861 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     4921 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     4981 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     5041 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     5101 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
     5161 ttgcatggcg gccgcataac ttcgtatagc atacattata cgaagttatg catgctcggt
     5221 gggtcaggtg gaagtggatc tggtgctatg atgggcagtg tcgagctgaa tctgagggag
     5281 actgagctgt gtcttggtct tcccggtgga gatacagtgg ctccggtaac cggaaacaag
     5341 agagggttct cagagacggt tgatctgaag ctaaatctga ataatgagcc tgcaaacaag
     5401 gaaggatcta cgactcatga cgtcgtgact tttgattcca aggagaagag tgcttgtcct
     5461 aaagatccag ccaaacctcc ggccaaggca caagttgtgg gatggccacc ggtgagatca
     5521 taccggaaga acgtgatggt ttcctgccaa aaatcaagcg gtggcccgga ggcggcggcg
     5581 ttcgtgaagg tatcaatgga cggagcaccg tacttgagga aaatcgattt gaggatgtat
     5641 aaaagctacg atgagctttc taatgctttg tccaacatgt tcagctcttt taccatgggc
     5701 aaacatggag gagaagaagg aatgatagac ttcatgaatg agaggaaatt gatggatttg
     5761 gtgaatagct gggactatgt tccctcttat gaagacaaag acggtgattg gatgctcgtc
     5821 ggcgacgttc cttggccaat gttcgtcgat acatgcaagc gtttacgtct catgaaagga
     5881 tcggatgcca ttggtctcgc tccgagggcg atggagaagt gcaagagcag agcttgagct
     5941 agcagtcccg gggcttgccc atctgtttta gacgtcacag aaggaccatg tttattttaa
     6001 atttcacata catgtgttta attaccttca ttgtataaat tctcgaaact ttctccaatt
     6061 cttcggtcag ataaaaaatt tctctagtca ccgtctttta tagagctttt gtcagatgtt
     6121 tttctggatg gaatcattgt ccagttacta tgctttttat ttataagttt gaaagtttag
     6181 ttaacttttt gttttcttta atacattttt taatcatgtc aacatttggg atttgtagaa
     6241 gtagtaaatt ttttgtacat gttgtagaat tggataagaa cgaaaacagc tagtatgtaa
     6301 aaaaaacgaa aatcccaaat tccgttgttt gtttgatata gtaatattta tagcatttta
     6361 gtttctaaag atttttgata atcttttaca tttgctttca catacattcg cgctactacc
     6421 atacatcata tcccgctttc tttaattgct tccccacaga ctcaaccagc ggggccttct
     6481 tctcgagatc tgtttagctt gcctcgtccc cgccgggtca cccggccagc gacatggagg
     6541 cccagaatac cctccttgac agtcttgacg tgcgcagctc aggggcatga tgtgactgtc
     6601 gcccgtacat ttagcccata catccccatg tataatcatt tgcatccata cattttgatg
     6661 gccgcacggc gcgaagcaaa aattacggct cctcgctgca gacctgcgag cagggaaacg
     6721 ctcccctcac agacgcgttg aattgtcccc acgccgcgcc cctgtagaga aatataaaag
     6781 gttaggattt gccactgagg ttcttctttc atatacttcc ttttaaaatc ttgctaggat
     6841 acagttctca catcacatcc gaacataaac aaccatgggt aaggaaaaga ctcacgtttc
     6901 gaggccgcga ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga
     6961 taatgtcggg caatcaggtg cgacaatcta tcgattgtat gggaagcccg atgcgccaga
     7021 gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag
     7081 actaaactgg ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc
     7141 tgatgatgca tggttactca ccactgcgat ccccggcaaa acagcattcc aggtattaga
     7201 agaatatcct gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt
     7261 gcattcgatt cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca
     7321 ggcgcaatca cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa
     7381 tggctggcct gttgaacaag tctggaaaga aatgcataag cttttgccat tctcaccgga
     7441 ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt
     7501 aataggttgt attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat
     7561 cctatggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata
     7621 tggtattgat aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt
     7681 ctaatcagta ctgacaataa aaagattctt gttttcaaga acttgtcatt tgtatagttt
     7741 ttttatattg tagttgttct attttaatca aatgttagcg tgatttatat tttttttcgc
     7801 ctcgacatca tctgcccaga tgcgaagtta agtgcgcaga aagtaatatc atgcgtcaat
     7861 cgtatgtgaa tgctggtcgc tatactgctg tcgattcgat actaacgccg ccatccagtc
     7921 gacgaactag tataacttcg tatatggtat tatatacgaa gttatgcggc cgcgcgaatt
     7981 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
     8041 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
     8101 gcccttccca acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg tattttctcc
     8161 ttacgcatct gtgcggtatt tcacaccgca taccatcgtc cggatcaatg aagtagatga
     8221 gtttactacc tgtatatact tttttttttg tcaaagtcac ttttatggca tttccatgtt
     8281 gaaggattag ccacagcact aaattttcag cgtatgattg cttttaaata tttaattttc
     8341 atcgtttttt aattaatatt caaaacgatt taatgccttc tacatctgaa gtcaagctca
     8401 tagactcggg ttgggtagct aaaactacat ctaacacgaa ggggtatagc cttcttaaag
     8461 tatactcatg tataaacgtt ttgggttcat atttttgggc cacgtggttt ataaaaattc
     8521 ttaactacac cactcggtat cccgcacccg tctacgtttc tacgatttcg agctaatatt
     8581 gagtaaagtg attagcaaaa aaaataaaag tactcgttgt cggagatcaa gaatttttct
     8641 attatctcat ctaaaccact ttctaaaagc gaaaaacaaa atcgtaatat gcagcttgaa
     8701 tgggcttcca tagtttgaaa gaaaaaccct agcagtactg gcaagggaga cattcctttt
     8761 acctggctaa tcgccatctt ccagcaggcg caccattgcc cctgtttcac tatccaggtt
     8821 acggatatag ttcatgacaa tatttacatt ggtccagcca ccagcttgca tgatctccgg
     8881 tattgaaact ccagcgcggg ccatatctcg cgcggctccg acacgggcac tgtgtccaga
     8941 ccaggccagg tatctctgac cagagtcatc cttagcgccg taaatcaatc gatgagttgc
     9001 ttcaaaaatc ccttccaggg cgcgagttga tagctggctg gtggcagatg gcgcggcaac
     9061 accatttttt ctgacccggc aaaacaggta gttattcgga tcatcagcta caccagagac
     9121 ggaaatccat cgctcgacca gtttagttac ccccaggcta agtgccttct ctacacctgc
     9181 ggtgctaacc agcgttttcg ttctgccaat atggattaac attctcccac cgtcagtacg
     9241 tgagatatct ttaaccctga tcctggcaat ttcggctata cgtaacaggg tgttataagc
     9301 aatccccaga aatgccagat tacgtatatc ctggcagcga tcgctatttt ccatgagtga
     9361 acgaacctgg tcgaaatcag tgcgttcgaa cgctagagcc tgttttgcac gttcaccggc
     9421 atcaacgttt tcttttcgga tccgccgcat aaccagtgaa acagcattgc tgtcacttgg
     9481 tcgtggcagc ccggaccgac gatgaagcat gtttagctgg cccaaatgtt gctggatagt
     9541 ttttactgcc agaccgcgcg cctgaagata tagaagataa tcgcgaacat cttcaggttc
     9601 tgcgggaaac catttccggt tattcaactt gcaccatgcc gcccacgacc ggcaaacgga
     9661 cagaagcatt ttccaggtat gctcagaaaa cgcctggcga tccctgaaca tgtccatcag
     9721 gttcttgcga acctcatcac tcgttgcatc gaccggtaac ttttagatta acatttgttt
     9781 aaatatgaaa ttaggcaatg taacatactg caggcaaatt ttggtgtacg gtaagtaaat
     9841 tggacatatg atttaacaaa gcgactataa gtcagaaagt gagaatgaga ttgaaataat
     9901 taattcaact tattcaattg atgatatgcc aggattcctc ttcctttatc ttcaatgttt
     9961 cgattaacag gtccattatc cggtttttag tttcgctgca cattgccgaa tgacaatcgg
    10021 attcctttaa gctttgcttt tgccctgttt tctctcccca gtaatcgttg ggtgactgaa
    10081 ccatccagta gccgagagtt acgtttttcc tgatttaata aatatccata tctatttgtc
    10141 tcgtggtgtt ttacagcaac taaccgaaat tactattttg catcatccaa ccataaagag
    10201 acaacaataa ttctagaaaa catataacaa gtgttttttt ctatttacgt ctcatacgca
    10261 aagtccactt taccatgttt tggaaacttg tacccaacca ttcatttttc gattttgtaa
    10321 tgaggctctt ttcttctaac aatcattgag gtcattatca tgtgtcctca cctattgttt
    10381 aaattgaggc ttgcgctatt cccaaaattg tgggtattat tttcatatat cagtatctgc
    10441 aatttcggtt caactccgga agctcggtac ccaactgtgg gaatactcag gtatcgtaag
    10501 atgcaagagt tcgaatctct tagcaaccat tatttttttt ctctcaacat aacgagaaca
    10561 cacaggggcg ctatcgcaca gaatcaaatt cgatgactgg aaattttttg ttaatttcag
    10621 aggtcgcctg acgcatatac ctttttcaac tgaaaaattg ggagaaaaag gaaaggtgag
    10681 agcgccggaa ccggcttttc atatagaata gagaagcgtt catgactaaa tgcttgcatc
    10741 acaatacttg aagttgacaa tattatttaa ggacctattg ttttttccaa taggtggtta
    10801 gcaatcgtct tactttctaa cttttcttac cttttacatt tcagcaatat atatatatat
    10861 atatatatat atttcaagga tataccattg taatgtctgc ccctaagaag atcgtcgttt
    10921 tgccaggtga ccacgttggt ca
//
