LOCUS       sequence-333203-       11221 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-terminal aid-3HA-spo5DSR degron cassette with kanMX6 marker for S.
            pombe.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11221)
  TITLE     Carr lab yeast expression plasmids
REFERENCE   2  (bases 1 to 11221)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAW8-aid-3HA-spo5DSR-Turg1:kanMX6
FEATURES             Location/Qualifiers
     source          1..11221
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      575..1356
                     /label=ars1
                     /note="Schizosaccharomyces pombe autonomously replicating 
                     sequence ars1"
     primer_bind     complement(1451..1470)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1570..1592
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1630..1648)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1716..1820
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1821..1889,1890..2681)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2039..2058)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2852..3440
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3341..3360
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3594..3611
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3728..3749
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3764..3769,3770..3787,3788..3794)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3802..3818
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3807..3829
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3826..3842
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3826..3842
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    complement(3874..3907)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             3946..4632
                     /codon_start=1
                     /gene="Arabidopsis thaliana AXR3"
                     /product="auxin-responsive IAA17 protein"
                     /label=auxin-inducible degron
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="MMGSVELNLRETELCLGLPGGDTVAPVTGNKRGFSETVDLKLNLN
                     NEPANKEGSTTHDVVTFDSKEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSS
                     GGPEAAAFVKVSMDGAPYLRKIDLRMYKSYDELSNALSNMFSSFTMGKHGGEEGMIDFM
                     NERKLMDLVNSWDYVPSYEDKDGDWMLVGDVPWPMFVDTCKRLRLMKGSDAIGLAPRAM
                     EKCKSRA"
     CDS             4651..4740
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     gene            5508..6864
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     promoter        5508..5851
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             5852..6661
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(5919..5938)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      6667..6864
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(6959..6976)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6959..6975)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6968..6990)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             9870..10964
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
ORIGIN
        1 caaaaagtta ggtgtaacag aaaatcatga aactatgatt tctaatttat atattggagg
       61 attttctcta aaaaaaaaaa tacaacaaat aaaaaacact caatgacctg accatttgat
      121 ggagtttaag tcaatacctt cttgaaccat ttcccataat ggtgaaagtt ccctcaagaa
      181 ttttactctg tcagaaacgg ccttaacgac gtagtcgatc cggacgatgg ccaaccttcc
      241 aattcattaa atcgagtcct ccatcagatg atttacaagg ttttgcatag aatccctaag
      301 taagagaagt tgttagtgat ttcttcaagt ttactaaaat ttacatacaa atggatgatc
      361 tcttctccat tgtttccttt cttcttgcaa acgcgtttta caaagagatg acatgctatt
      421 ttctgattat ttttttctat agttttctat tttgtacgtc cttgggagtc cgaaatgtaa
      481 aatcggggta tggttagtag cggcagcgtt cattatggta tataaaagat aaaaagtagg
      541 ggcaagcgag acagaatggg atacaagggc atcgtctata attatagcta aaaattgtat
      601 tttaatttgt attttttgta attttatttc tcattgtttt acttaaaatg aatgcgaatt
      661 agagaaaact tcaacgaaat gtcaaaataa gctcagcaaa atatacaatt ttagggaaag
      721 cgatcagcaa cttattctcc gttgtagatt ctttttcttc ataaattcca acaatgtaac
      781 tgccaattga tttttctcta gatgctaata aattgaaact aatgaatata ataacacagg
      841 ttttgataaa ttgtaaactc ttactcaaaa aaagaatgtt aaaactaatt taaccttttt
      901 ataaaggctc atggcgttaa agaggatagt aaaaattcta caatttttta atttaatgtc
      961 acaattgtct tgcgaatata attctattaa ttttcgctaa ctgctgtttt agtactatta
     1021 ctttcaatat gccttatatg caactttaat tcatgaagaa tgcaatattt ttgcaatctt
     1081 tcttgtactg agcgcttttt atcatttagt actcattatt taatttttca gtaaagggca
     1141 ataaataaat ttgtagaaga tgcaatgtaa tctcctctat ccttttgctc atatgtttat
     1201 gagtatacct agtctagaaa ggcttgtatt taaaatatta ttcaataaaa attcacaatt
     1261 tttacgacat gtgctaccat tcacttaact tcctgattat aaaattggtt cgtttatact
     1321 aattacttaa gtacctttaa ctaaacaaaa tgcctatata tatattaatt tacaatgagt
     1381 gtcagataag tcactatgtc cgagtggtta aggagttaga ctcgccatcg atggtatggt
     1441 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
     1501 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     1561 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     1621 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     1681 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     1741 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     1801 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     1861 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     1921 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     1981 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     2041 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     2101 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     2161 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     2221 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     2281 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     2341 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     2401 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     2461 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     2521 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     2581 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     2641 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     2701 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     2761 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     2821 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     2881 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     2941 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     3001 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     3061 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     3121 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     3181 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     3241 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     3301 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     3361 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     3421 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     3481 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     3541 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     3601 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     3661 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     3721 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     3781 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     3841 accatgatta cgccaagctt gcatggcggc cgcataactt cgtatagcat acattatacg
     3901 aagttatgca tgctcggtgg gtcaggtgga agtggatctg gtgctatgat gggcagtgtc
     3961 gagctgaatc tgagggagac tgagctgtgt cttggtcttc ccggtggaga tacagtggct
     4021 ccggtaaccg gaaacaagag agggttctca gagacggttg atctgaagct aaatctgaat
     4081 aatgagcctg caaacaagga aggatctacg actcatgacg tcgtgacttt tgattccaag
     4141 gagaagagtg cttgtcctaa agatccagcc aaacctccgg ccaaggcaca agttgtggga
     4201 tggccaccgg tgagatcata ccggaagaac gtgatggttt cctgccaaaa atcaagcggt
     4261 ggcccggagg cggcggcgtt cgtgaaggta tcaatggacg gagcaccgta cttgaggaaa
     4321 atcgatttga ggatgtataa aagctacgat gagctttcta atgctttgtc caacatgttc
     4381 agctctttta ccatgggcaa acatggagga gaagaaggaa tgatagactt catgaatgag
     4441 aggaaattga tggatttggt gaatagctgg gactatgttc cctcttatga agacaaagac
     4501 ggtgattgga tgctcgtcgg cgacgttcct tggccaatgt tcgtcgatac atgcaagcgt
     4561 ttacgtctca tgaaaggatc ggatgccatt ggtctcgctc cgagggcgat ggagaagtgc
     4621 aagagcagag ctggtgctgg agcaggcgcc tacccatacg atgttcctga ctatgcgggc
     4681 tatccctatg acgtcccgga ctatgcagga tcctatccat atgacgttcc agattacgct
     4741 gctcagtgct gaggcgcgcc gctagcacta cgccatatca tgcccatccc gctacttctg
     4801 ctgctaatta aatttaaaca tctttccagc atttacttga acatcaaact ctaatatcga
     4861 aatcttttca aacacttatc acatactcaa acttaaacca acataaaacc tgttagacaa
     4921 agccccgggg cttgcccatc tgttttagac gtcacagaag gaccatgttt attttaaatt
     4981 tcacatacat gtgtttaatt accttcattg tataaattct cgaaactttc tccaattctt
     5041 cggtcagata aaaaatttct ctagtcaccg tcttttatag agcttttgtc agatgttttt
     5101 ctggatggaa tcattgtcca gttactatgc tttttattta taagtttgaa agtttagtta
     5161 actttttgtt ttctttaata cattttttaa tcatgtcaac atttgggatt tgtagaagta
     5221 gtaaattttt tgtacatgtt gtagaattgg ataagaacga aaacagctag tatgtaaaaa
     5281 aaacgaaaat cccaaattcc gttgtttgtt tgatatagta atatttatag cattttagtt
     5341 tctaaagatt tttgataatc ttttacattt gctttcacat acattcgcgc tactaccata
     5401 catcatatcc cgctttcttt aattgcttcc ccacagactc aaccagcggg gccttcttct
     5461 cgagatctgt ttagcttgcc tcgtccccgc cgggtcaccc ggccagcgac atggaggccc
     5521 agaataccct ccttgacagt cttgacgtgc gcagctcagg ggcatgatgt gactgtcgcc
     5581 cgtacattta gcccatacat ccccatgtat aatcatttgc atccatacat tttgatggcc
     5641 gcacggcgcg aagcaaaaat tacggctcct cgctgcagac ctgcgagcag ggaaacgctc
     5701 ccctcacaga cgcgttgaat tgtccccacg ccgcgcccct gtagagaaat ataaaaggtt
     5761 aggatttgcc actgaggttc ttctttcata tacttccttt taaaatcttg ctaggataca
     5821 gttctcacat cacatccgaa cataaacaac catgggtaag gaaaagactc acgtttcgag
     5881 gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa
     5941 tgtcgggcaa tcaggtgcga caatctatcg attgtatggg aagcccgatg cgccagagtt
     6001 gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact
     6061 aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga
     6121 tgatgcatgg ttactcacca ctgcgatccc cggcaaaaca gcattccagg tattagaaga
     6181 atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca
     6241 ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc
     6301 gcaatcacga atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg
     6361 ctggcctgtt gaacaagtct ggaaagaaat gcataagctt ttgccattct caccggattc
     6421 agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat
     6481 aggttgtatt gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct
     6541 atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg
     6601 tattgataat cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta
     6661 atcagtactg acaataaaaa gattcttgtt ttcaagaact tgtcatttgt atagtttttt
     6721 tatattgtag ttgttctatt ttaatcaaat gttagcgtga tttatatttt ttttcgcctc
     6781 gacatcatct gcccagatgc gaagttaagt gcgcagaaag taatatcatg cgtcaatcgt
     6841 atgtgaatgc tggtcgctat actgctgtcg attcgatact aacgccgcca tccagtcgac
     6901 gaactagtat aacttcgtat atggtattat atacgaagtt atgcggccgc gcgaattcac
     6961 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
     7021 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     7081 cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat tttctcctta
     7141 cgcatctgtg cggtatttca caccgcatac catcgtccgg atcaatgaag tagatgagtt
     7201 tactacctgt atatactttt ttttttgtca aagtcacttt tatggcattt ccatgttgaa
     7261 ggattagcca cagcactaaa ttttcagcgt atgattgctt ttaaatattt aattttcatc
     7321 gttttttaat taatattcaa aacgatttaa tgccttctac atctgaagtc aagctcatag
     7381 actcgggttg ggtagctaaa actacatcta acacgaaggg gtatagcctt cttaaagtat
     7441 actcatgtat aaacgttttg ggttcatatt tttgggccac gtggtttata aaaattctta
     7501 actacaccac tcggtatccc gcacccgtct acgtttctac gatttcgagc taatattgag
     7561 taaagtgatt agcaaaaaaa ataaaagtac tcgttgtcgg agatcaagaa tttttctatt
     7621 atctcatcta aaccactttc taaaagcgaa aaacaaaatc gtaatatgca gcttgaatgg
     7681 gcttccatag tttgaaagaa aaaccctagc agtactggca agggagacat tccttttacc
     7741 tggctaatcg ccatcttcca gcaggcgcac cattgcccct gtttcactat ccaggttacg
     7801 gatatagttc atgacaatat ttacattggt ccagccacca gcttgcatga tctccggtat
     7861 tgaaactcca gcgcgggcca tatctcgcgc ggctccgaca cgggcactgt gtccagacca
     7921 ggccaggtat ctctgaccag agtcatcctt agcgccgtaa atcaatcgat gagttgcttc
     7981 aaaaatccct tccagggcgc gagttgatag ctggctggtg gcagatggcg cggcaacacc
     8041 attttttctg acccggcaaa acaggtagtt attcggatca tcagctacac cagagacgga
     8101 aatccatcgc tcgaccagtt tagttacccc caggctaagt gccttctcta cacctgcggt
     8161 gctaaccagc gttttcgttc tgccaatatg gattaacatt ctcccaccgt cagtacgtga
     8221 gatatcttta accctgatcc tggcaatttc ggctatacgt aacagggtgt tataagcaat
     8281 ccccagaaat gccagattac gtatatcctg gcagcgatcg ctattttcca tgagtgaacg
     8341 aacctggtcg aaatcagtgc gttcgaacgc tagagcctgt tttgcacgtt caccggcatc
     8401 aacgttttct tttcggatcc gccgcataac cagtgaaaca gcattgctgt cacttggtcg
     8461 tggcagcccg gaccgacgat gaagcatgtt tagctggccc aaatgttgct ggatagtttt
     8521 tactgccaga ccgcgcgcct gaagatatag aagataatcg cgaacatctt caggttctgc
     8581 gggaaaccat ttccggttat tcaacttgca ccatgccgcc cacgaccggc aaacggacag
     8641 aagcattttc caggtatgct cagaaaacgc ctggcgatcc ctgaacatgt ccatcaggtt
     8701 cttgcgaacc tcatcactcg ttgcatcgac cggtaacttt tagattaaca tttgtttaaa
     8761 tatgaaatta ggcaatgtaa catactgcag gcaaattttg gtgtacggta agtaaattgg
     8821 acatatgatt taacaaagcg actataagtc agaaagtgag aatgagattg aaataattaa
     8881 ttcaacttat tcaattgatg atatgccagg attcctcttc ctttatcttc aatgtttcga
     8941 ttaacaggtc cattatccgg tttttagttt cgctgcacat tgccgaatga caatcggatt
     9001 cctttaagct ttgcttttgc cctgttttct ctccccagta atcgttgggt gactgaacca
     9061 tccagtagcc gagagttacg tttttcctga tttaataaat atccatatct atttgtctcg
     9121 tggtgtttta cagcaactaa ccgaaattac tattttgcat catccaacca taaagagaca
     9181 acaataattc tagaaaacat ataacaagtg tttttttcta tttacgtctc atacgcaaag
     9241 tccactttac catgttttgg aaacttgtac ccaaccattc atttttcgat tttgtaatga
     9301 ggctcttttc ttctaacaat cattgaggtc attatcatgt gtcctcacct attgtttaaa
     9361 ttgaggcttg cgctattccc aaaattgtgg gtattatttt catatatcag tatctgcaat
     9421 ttcggttcaa ctccggaagc tcggtaccca actgtgggaa tactcaggta tcgtaagatg
     9481 caagagttcg aatctcttag caaccattat tttttttctc tcaacataac gagaacacac
     9541 aggggcgcta tcgcacagaa tcaaattcga tgactggaaa ttttttgtta atttcagagg
     9601 tcgcctgacg catatacctt tttcaactga aaaattggga gaaaaaggaa aggtgagagc
     9661 gccggaaccg gcttttcata tagaatagag aagcgttcat gactaaatgc ttgcatcaca
     9721 atacttgaag ttgacaatat tatttaagga cctattgttt tttccaatag gtggttagca
     9781 atcgtcttac tttctaactt ttcttacctt ttacatttca gcaatatata tatatatata
     9841 tatatatatt tcaaggatat accattgtaa tgtctgcccc taagaagatc gtcgttttgc
     9901 caggtgacca cgttggtcaa gaaatcacag ccgaagccat taaggttctt aaagctattt
     9961 ctgatgttcg ttccaatgtc aagttcgatt tcgaaaatca tttaattggt ggtgctgcta
    10021 tcgatgctac aggtgttcca cttccagatg aggcgctgga agcctccaag aaggctgatg
    10081 ccgttttgtt aggtgctgtg ggtggtccta aatggggtac cggtagtgtt agacctgaac
    10141 aaggtttact aaaaatccgt aaagaacttc aattgtacgc caacttaaga ccatgtaact
    10201 ttgcatccga ctctctttta gacttatctc caatcaagcc acaatttgct aaaggtactg
    10261 acttcgttgt tgttagagaa ttagtgggag gtatttactt tggtaagaga aaggaagacg
    10321 atggtgatgg tgtcgcttgg gatagtgaac aatacaccgt tccagaagtg caaagaatca
    10381 caagaatggc cgctttcatg gccctacaac atgagccacc attgcctatt tggtccttgg
    10441 ataaagctaa tgttttggcc tcttcaagat tatggagaaa aactgtggag gaaaccatca
    10501 agaacgaatt tcctacattg aaagttcaac atcaattgat tgattctgcc gccatgatcc
    10561 tagttaagaa cccaacccac ctaaatggta ttataatcac cagcaacatg tttggtgata
    10621 tcatctccga tgaagcctcc gttatcccag gctccttggg tttgttgcca tctgcgtcct
    10681 tggcctcttt gccagacaag aacaccgcat ttggtttgta cgaaccatgc catggttccg
    10741 ctccagattt gccaaagaat aaggtcaacc ctatcgccac tatcttgtct gctgcaatga
    10801 tgttgaaatt gtcattgaac ttgcctgaag aaggtaaagc cattgaagat gcagttaaaa
    10861 aggttttgga tgcaggtatc agaactggtg atttaggtgg ttccaacagt accaccgaag
    10921 tcggtgatgc tgtcgccgaa gaagttaaga aaatccttgc ttaaaaagat tctctttttt
    10981 tgtgatattt gtacataaac tttataaatg aaattcataa tagaaacgac acgaaattac
    11041 aaaatggaat atgttcatag ggtagacgaa actatatacg caatctacat acatttatca
    11101 agaaggagaa aaaggaggat gtaaaggaat acaggtaagc aaattgatac taatggctca
    11161 acgtgataag gaaaaagaat tgcactttaa cattaatatt gacaaggagg agggcatcac
    11221 a
//
