LOCUS       sequence-333204-       11148 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-terminal aid-5Myc degron cassette with kanMX6 marker for S. pombe.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11148)
  TITLE     Carr lab yeast expression plasmids
REFERENCE   2  (bases 1 to 11148)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAW8-aid-5Myc-Turg1:kanMX6
FEATURES             Location/Qualifiers
     source          1..11148
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      575..1356
                     /label=ars1
                     /note="Schizosaccharomyces pombe autonomously replicating 
                     sequence ars1"
     primer_bind     complement(1451..1470)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1570..1592
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1630..1648)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1716..1820
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1821..1889,1890..2681)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2039..2058)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2852..3440
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3341..3360
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3594..3611
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3728..3749
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3764..3769,3770..3787,3788..3794)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3802..3818
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3807..3829
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3826..3842
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3826..3842
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    complement(3874..3907)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             3946..4632
                     /codon_start=1
                     /gene="Arabidopsis thaliana AXR3"
                     /product="auxin-responsive IAA17 protein"
                     /label=auxin-inducible degron
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="MMGSVELNLRETELCLGLPGGDTVAPVTGNKRGFSETVDLKLNLN
                     NEPANKEGSTTHDVVTFDSKEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSS
                     GGPEAAAFVKVSMDGAPYLRKIDLRMYKSYDELSNALSNMFSSFTMGKHGGEEGMIDFM
                     NERKLMDLVNSWDYVPSYEDKDGDWMLVGDVPWPMFVDTCKRLRLMKGSDAIGLAPRAM
                     EKCKSRA"
     CDS             4648..4677
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4684..4713
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4720..4749
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4765..4794
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4801..4830
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     gene            5435..6791
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     promoter        5435..5778
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             5779..6588
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(5846..5865)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      6594..6791
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(6886..6903)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6886..6902)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6895..6917)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             9797..10891
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
ORIGIN
        1 caaaaagtta ggtgtaacag aaaatcatga aactatgatt tctaatttat atattggagg
       61 attttctcta aaaaaaaaaa tacaacaaat aaaaaacact caatgacctg accatttgat
      121 ggagtttaag tcaatacctt cttgaaccat ttcccataat ggtgaaagtt ccctcaagaa
      181 ttttactctg tcagaaacgg ccttaacgac gtagtcgatc cggacgatgg ccaaccttcc
      241 aattcattaa atcgagtcct ccatcagatg atttacaagg ttttgcatag aatccctaag
      301 taagagaagt tgttagtgat ttcttcaagt ttactaaaat ttacatacaa atggatgatc
      361 tcttctccat tgtttccttt cttcttgcaa acgcgtttta caaagagatg acatgctatt
      421 ttctgattat ttttttctat agttttctat tttgtacgtc cttgggagtc cgaaatgtaa
      481 aatcggggta tggttagtag cggcagcgtt cattatggta tataaaagat aaaaagtagg
      541 ggcaagcgag acagaatggg atacaagggc atcgtctata attatagcta aaaattgtat
      601 tttaatttgt attttttgta attttatttc tcattgtttt acttaaaatg aatgcgaatt
      661 agagaaaact tcaacgaaat gtcaaaataa gctcagcaaa atatacaatt ttagggaaag
      721 cgatcagcaa cttattctcc gttgtagatt ctttttcttc ataaattcca acaatgtaac
      781 tgccaattga tttttctcta gatgctaata aattgaaact aatgaatata ataacacagg
      841 ttttgataaa ttgtaaactc ttactcaaaa aaagaatgtt aaaactaatt taaccttttt
      901 ataaaggctc atggcgttaa agaggatagt aaaaattcta caatttttta atttaatgtc
      961 acaattgtct tgcgaatata attctattaa ttttcgctaa ctgctgtttt agtactatta
     1021 ctttcaatat gccttatatg caactttaat tcatgaagaa tgcaatattt ttgcaatctt
     1081 tcttgtactg agcgcttttt atcatttagt actcattatt taatttttca gtaaagggca
     1141 ataaataaat ttgtagaaga tgcaatgtaa tctcctctat ccttttgctc atatgtttat
     1201 gagtatacct agtctagaaa ggcttgtatt taaaatatta ttcaataaaa attcacaatt
     1261 tttacgacat gtgctaccat tcacttaact tcctgattat aaaattggtt cgtttatact
     1321 aattacttaa gtacctttaa ctaaacaaaa tgcctatata tatattaatt tacaatgagt
     1381 gtcagataag tcactatgtc cgagtggtta aggagttaga ctcgccatcg atggtatggt
     1441 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
     1501 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     1561 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     1621 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggtty
     1681 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     1741 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     1801 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     1861 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     1921 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     1981 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     2041 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     2101 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     2161 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     2221 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     2281 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     2341 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     2401 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     2461 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     2521 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     2581 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     2641 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     2701 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     2761 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     2821 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     2881 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     2941 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     3001 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     3061 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     3121 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     3181 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     3241 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     3301 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     3361 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     3421 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     3481 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     3541 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     3601 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     3661 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     3721 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     3781 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     3841 accatgatta cgccaagctt gcatggcggc cgcataactt cgtatagcat acattatacg
     3901 aagttatgca tgctcggtgg gtcaggtgga agtggatctg gtgctatgat gggcagtgtc
     3961 gagctgaatc tgagggagac tgagctgtgt cttggtcttc ccggtggaga tacagtggct
     4021 ccggtaaccg gaaacaagag agggttctca gagacggttg atctgaagct aaatctgaat
     4081 aatgagcctg caaacaagga aggatctacg actcatgacg tcgtgacttt tgattccaag
     4141 gagaagagtg cttgtcctaa agatccagcc aaacctccgg ccaaggcaca agttgtggga
     4201 tggccaccgg tgagatcata ccggaagaac gtgatggttt cctgccaaaa atcaagcggt
     4261 ggcccggagg cggcggcgtt cgtgaaggta tcaatggacg gagcaccgta cttgaggaaa
     4321 atcgatttga ggatgtataa aagctacgat gagctttcta atgctttgtc caacatgttc
     4381 agctctttta ccatgggcaa acatggagga gaagaaggaa tgatagactt catgaatgag
     4441 aggaaattga tggatttggt gaatagctgg gactatgttc cctcttatga agacaaagac
     4501 ggtgattgga tgctcgtcgg cgacgttcct tggccaatgt tcgtcgatac atgcaagcgt
     4561 ttacgtctca tgaaaggatc ggatgccatt ggtctcgctc cgagggcgat ggagaagtgc
     4621 aagagcagag ctggagcagg cgccggtgaa caaaagttga tttctgaaga agatttgaac
     4681 ggtgaacaaa agctaatctc cgaggaagac ttgaacggtg aacaaaaatt aatctcagaa
     4741 gaagacttga acggactcga cggtgaacaa aagttgattt ctgaagaaga tttgaacggt
     4801 gaacaaaagc taatctccga ggaagacttg taaggcgcgc cgctagcagt cccggggctt
     4861 gcccatctgt tttagacgtc acagaaggac catgtttatt ttaaatttca catacatgtg
     4921 tttaattacc ttcattgtat aaattctcga aactttctcc aattcttcgg tcagataaaa
     4981 aatttctcta gtcaccgtct tttatagagc ttttgtcaga tgtttttctg gatggaatca
     5041 ttgtccagtt actatgcttt ttatttataa gtttgaaagt ttagttaact ttttgttttc
     5101 tttaatacat tttttaatca tgtcaacatt tgggatttgt agaagtagta aattttttgt
     5161 acatgttgta gaattggata agaacgaaaa cagctagtat gtaaaaaaaa cgaaaatccc
     5221 aaattccgtt gtttgtttga tatagtaata tttatagcat tttagtttct aaagattttt
     5281 gataatcttt tacatttgct ttcacataca ttcgcgctac taccatacat catatcccgc
     5341 tttctttaat tgcttcccca cagactcaac cagcggggcc ttcttctcga gatctgttta
     5401 gcttgcctcg tccccgccgg gtcacccggc cagcgacatg gaggcccaga ataccctcct
     5461 tgacagtctt gacgtgcgca gctcaggggc atgatgtgac tgtcgcccgt acatttagcc
     5521 catacatccc catgtataat catttgcatc catacatttt gatggccgca cggcgcgaag
     5581 caaaaattac ggctcctcgc tgcagacctg cgagcaggga aacgctcccc tcacagacgc
     5641 gttgaattgt ccccacgccg cgcccctgta gagaaatata aaaggttagg atttgccact
     5701 gaggttcttc tttcatatac ttccttttaa aatcttgcta ggatacagtt ctcacatcac
     5761 atccgaacat aaacaaccat gggtaaggaa aagactcacg tttcgaggcc gcgattaaat
     5821 tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca
     5881 ggtgcgacaa tctatcgatt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat
     5941 ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg
     6001 gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta
     6061 ctcaccactg cgatccccgg caaaacagca ttccaggtat tagaagaata tcctgattca
     6121 ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt
     6181 tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg
     6241 aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa
     6301 caagtctgga aagaaatgca taagcttttg ccattctcac cggattcagt cgtcactcat
     6361 ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat
     6421 gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc
     6481 ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct
     6541 gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc agtactgaca
     6601 ataaaaagat tcttgttttc aagaacttgt catttgtata gtttttttat attgtagttg
     6661 ttctatttta atcaaatgtt agcgtgattt atattttttt tcgcctcgac atcatctgcc
     6721 cagatgcgaa gttaagtgcg cagaaagtaa tatcatgcgt caatcgtatg tgaatgctgg
     6781 tcgctatact gctgtcgatt cgatactaac gccgccatcc agtcgacgaa ctagtataac
     6841 ttcgtatatg gtattatata cgaagttatg cggccgcgcg aattcactgg ccgtcgtttt
     6901 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
     6961 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
     7021 gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg
     7081 tatttcacac cgcataccat cgtccggatc aatgaagtag atgagtttac tacctgtata
     7141 tacttttttt tttgtcaaag tcacttttat ggcatttcca tgttgaagga ttagccacag
     7201 cactaaattt tcagcgtatg attgctttta aatatttaat tttcatcgtt ttttaattaa
     7261 tattcaaaac gatttaatgc cttctacatc tgaagtcaag ctcatagact cgggttgggt
     7321 agctaaaact acatctaaca cgaaggggta tagccttctt aaagtatact catgtataaa
     7381 cgttttgggt tcatattttt gggccacgtg gtttataaaa attcttaact acaccactcg
     7441 gtatcccgca cccgtctacg tttctacgat ttcgagctaa tattgagtaa agtgattagc
     7501 aaaaaaaata aaagtactcg ttgtcggaga tcaagaattt ttctattatc tcatctaaac
     7561 cactttctaa aagcgaaaaa caaaatcgta atatgcagct tgaatgggct tccatagttt
     7621 gaaagaaaaa ccctagcagt actggcaagg gagacattcc ttttacctgg ctaatcgcca
     7681 tcttccagca ggcgcaccat tgcccctgtt tcactatcca ggttacggat atagttcatg
     7741 acaatattta cattggtcca gccaccagct tgcatgatct ccggtattga aactccagcg
     7801 cgggccatat ctcgcgcggc tccgacacgg gcactgtgtc cagaccaggc caggtatctc
     7861 tgaccagagt catccttagc gccgtaaatc aatcgatgag ttgcttcaaa aatcccttcc
     7921 agggcgcgag ttgatagctg gctggtggca gatggcgcgg caacaccatt ttttctgacc
     7981 cggcaaaaca ggtagttatt cggatcatca gctacaccag agacggaaat ccatcgctcg
     8041 accagtttag ttacccccag gctaagtgcc ttctctacac ctgcggtgct aaccagcgtt
     8101 ttcgttctgc caatatggat taacattctc ccaccgtcag tacgtgagat atctttaacc
     8161 ctgatcctgg caatttcggc tatacgtaac agggtgttat aagcaatccc cagaaatgcc
     8221 agattacgta tatcctggca gcgatcgcta ttttccatga gtgaacgaac ctggtcgaaa
     8281 tcagtgcgtt cgaacgctag agcctgtttt gcacgttcac cggcatcaac gttttctttt
     8341 cggatccgcc gcataaccag tgaaacagca ttgctgtcac ttggtcgtgg cagcccggac
     8401 cgacgatgaa gcatgtttag ctggcccaaa tgttgctgga tagtttttac tgccagaccg
     8461 cgcgcctgaa gatatagaag ataatcgcga acatcttcag gttctgcggg aaaccatttc
     8521 cggttattca acttgcacca tgccgcccac gaccggcaaa cggacagaag cattttccag
     8581 gtatgctcag aaaacgcctg gcgatccctg aacatgtcca tcaggttctt gcgaacctca
     8641 tcactcgttg catcgaccgg taacttttag attaacattt gtttaaatat gaaattaggc
     8701 aatgtaacat actgcaggca aattttggtg tacggtaagt aaattggaca tatgatttaa
     8761 caaagcgact ataagtcaga aagtgagaat gagattgaaa taattaattc aacttattca
     8821 attgatgata tgccaggatt cctcttcctt tatcttcaat gtttcgatta acaggtccat
     8881 tatccggttt ttagtttcgc tgcacattgc cgaatgacaa tcggattcct ttaagctttg
     8941 cttttgccct gttttctctc cccagtaatc gttgggtgac tgaaccatcc agtagccgag
     9001 agttacgttt ttcctgattt aataaatatc catatctatt tgtctcgtgg tgttttacag
     9061 caactaaccg aaattactat tttgcatcat ccaaccataa agagacaaca ataattctag
     9121 aaaacatata acaagtgttt ttttctattt acgtctcata cgcaaagtcc actttaccat
     9181 gttttggaaa cttgtaccca accattcatt tttcgatttt gtaatgaggc tcttttcttc
     9241 taacaatcat tgaggtcatt atcatgtgtc ctcacctatt gtttaaattg aggcttgcgc
     9301 tattcccaaa attgtgggta ttattttcat atatcagtat ctgcaatttc ggttcaactc
     9361 cggaagctcg gtacccaact gtgggaatac tcaggtatcg taagatgcaa gagttcgaat
     9421 ctcttagcaa ccattatttt ttttctctca acataacgag aacacacagg ggcgctatcg
     9481 cacagaatca aattcgatga ctggaaattt tttgttaatt tcagaggtcg cctgacgcat
     9541 ataccttttt caactgaaaa attgggagaa aaaggaaagg tgagagcgcc ggaaccggct
     9601 tttcatatag aatagagaag cgttcatgac taaatgcttg catcacaata cttgaagttg
     9661 acaatattat ttaaggacct attgtttttt ccaataggtg gttagcaatc gtcttacttt
     9721 ctaacttttc ttacctttta catttcagca atatatatat atatatatat atatatttca
     9781 aggatatacc attgtaatgt ctgcccctaa gaagatcgtc gttttgccag gtgaccacgt
     9841 tggtcaagaa atcacagccg aagccattaa ggttcttaaa gctatttctg atgttcgttc
     9901 caatgtcaag ttcgatttcg aaaatcattt aattggtggt gctgctatcg atgctacagg
     9961 tgttccactt ccagatgagg cgctggaagc ctccaagaag gctgatgccg ttttgttagg
    10021 tgctgtgggt ggtcctaaat ggggtaccgg tagtgttaga cctgaacaag gtttactaaa
    10081 aatccgtaaa gaacttcaat tgtacgccaa cttaagacca tgtaactttg catccgactc
    10141 tcttttagac ttatctccaa tcaagccaca atttgctaaa ggtactgact tcgttgttgt
    10201 tagagaatta gtgggaggta tttactttgg taagagaaag gaagacgatg gtgatggtgt
    10261 cgcttgggat agtgaacaat acaccgttcc agaagtgcaa agaatcacaa gaatggccgc
    10321 tttcatggcc ctacaacatg agccaccatt gcctatttgg tccttggata aagctaatgt
    10381 tttggcctct tcaagattat ggagaaaaac tgtggaggaa accatcaaga acgaatttcc
    10441 tacattgaaa gttcaacatc aattgattga ttctgccgcc atgatcctag ttaagaaccc
    10501 aacccaccta aatggtatta taatcaccag caacatgttt ggtgatatca tctccgatga
    10561 agcctccgtt atcccaggct ccttgggttt gttgccatct gcgtccttgg cctctttgcc
    10621 agacaagaac accgcatttg gtttgtacga accatgccat ggttccgctc cagatttgcc
    10681 aaagaataag gtcaacccta tcgccactat cttgtctgct gcaatgatgt tgaaattgtc
    10741 attgaacttg cctgaagaag gtaaagccat tgaagatgca gttaaaaagg ttttggatgc
    10801 aggtatcaga actggtgatt taggtggttc caacagtacc accgaagtcg gtgatgctgt
    10861 cgccgaagaa gttaagaaaa tccttgctta aaaagattct ctttttttgt gatatttgta
    10921 cataaacttt ataaatgaaa ttcataatag aaacgacacg aaattacaaa atggaatatg
    10981 ttcatagggt agacgaaact atatacgcaa tctacataca tttatcaaga aggagaaaaa
    11041 ggaggatgta aaggaataca ggtaagcaaa ttgatactaa tggctcaacg tgataaggaa
    11101 aaagaattgc actttaacat taatattgac aaggaggagg gcatcaca
//
