LOCUS       sequence-333206-       11067 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-terminal aid-3HA degron cassette with kanMX6 marker for S. pombe.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11067)
  TITLE     Carr lab yeast expression plasmids
REFERENCE   2  (bases 1 to 11067)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAW8-aid-3HA-Turg1:kanMX6
FEATURES             Location/Qualifiers
     source          1..11067
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      575..1356
                     /label=ars1
                     /note="Schizosaccharomyces pombe autonomously replicating 
                     sequence ars1"
     primer_bind     complement(1451..1470)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1570..1592
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1630..1648)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1716..1820
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1821..1889,1890..2681)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2039..2058)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2852..3440
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3341..3360
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3594..3611
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3728..3749
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3764..3769,3770..3787,3788..3794)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3802..3818
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3807..3829
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3826..3842
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3826..3842
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    complement(3874..3907)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             3946..4632
                     /codon_start=1
                     /gene="Arabidopsis thaliana AXR3"
                     /product="auxin-responsive IAA17 protein"
                     /label=auxin-inducible degron
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="MMGSVELNLRETELCLGLPGGDTVAPVTGNKRGFSETVDLKLNLN
                     NEPANKEGSTTHDVVTFDSKEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSS
                     GGPEAAAFVKVSMDGAPYLRKIDLRMYKSYDELSNALSNMFSSFTMGKHGGEEGMIDFM
                     NERKLMDLVNSWDYVPSYEDKDGDWMLVGDVPWPMFVDTCKRLRLMKGSDAIGLAPRAM
                     EKCKSRA"
     CDS             4651..4740
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     gene            5354..6710
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     promoter        5354..5697
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             5698..6507
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(5765..5784)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      6513..6710
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(6805..6822)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6805..6821)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6814..6836)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             9716..10810
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
ORIGIN
        1 caaaaagtta ggtgtaacag aaaatcatga aactatgatt tctaatttat atattggagg
       61 attttctcta aaaaaaaaaa tacaacaaat aaaaaacact caatgacctg accatttgat
      121 ggagtttaag tcaatacctt cttgaaccat ttcccataat ggtgaaagtt ccctcaagaa
      181 ttttactctg tcagaaacgg ccttaacgac gtagtcgatc cggacgatgg ccaaccttcc
      241 aattcattaa atcgagtcct ccatcagatg atttacaagg ttttgcatag aatccctaag
      301 taagagaagt tgttagtgat ttcttcaagt ttactaaaat ttacatacaa atggatgatc
      361 tcttctccat tgtttccttt cttcttgcaa acgcgtttta caaagagatg acatgctatt
      421 ttctgattat ttttttctat agttttctat tttgtacgtc cttgggagtc cgaaatgtaa
      481 aatcggggta tggttagtag cggcagcgtt cattatggta tataaaagat aaaaagtagg
      541 ggcaagcgag acagaatggg atacaagggc atcgtctata attatagcta aaaattgtat
      601 tttaatttgt attttttgta attttatttc tcattgtttt acttaaaatg aatgcgaatt
      661 agagaaaact tcaacgaaat gtcaaaataa gctcagcaaa atatacaatt ttagggaaag
      721 cgatcagcaa cttattctcc gttgtagatt ctttttcttc ataaattcca acaatgtaac
      781 tgccaattga tttttctcta gatgctaata aattgaaact aatgaatata ataacacagg
      841 ttttgataaa ttgtaaactc ttactcaaaa aaagaatgtt aaaactaatt taaccttttt
      901 ataaaggctc atggcgttaa agaggatagt aaaaattcta caatttttta atttaatgtc
      961 acaattgtct tgcgaatata attctattaa ttttcgctaa ctgctgtttt agtactatta
     1021 ctttcaatat gccttatatg caactttaat tcatgaagaa tgcaatattt ttgcaatctt
     1081 tcttgtactg agcgcttttt atcatttagt actcattatt taatttttca gtaaagggca
     1141 ataaataaat ttgtagaaga tgcaatgtaa tctcctctat ccttttgctc atatgtttat
     1201 gagtatacct agtctagaaa ggcttgtatt taaaatatta ttcaataaaa attcacaatt
     1261 tttacgacat gtgctaccat tcacttaact tcctgattat aaaattggtt cgtttatact
     1321 aattacttaa gtacctttaa ctaaacaaaa tgcctatata tatattaatt tacaatgagt
     1381 gtcagataag tcactatgtc cgagtggtta aggagttaga ctcgccatcg atggtatggt
     1441 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
     1501 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     1561 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     1621 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     1681 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     1741 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     1801 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     1861 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     1921 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     1981 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     2041 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     2101 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     2161 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     2221 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     2281 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     2341 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     2401 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     2461 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     2521 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     2581 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     2641 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     2701 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     2761 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     2821 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     2881 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     2941 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     3001 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     3061 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     3121 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     3181 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     3241 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     3301 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     3361 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     3421 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     3481 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     3541 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     3601 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     3661 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     3721 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     3781 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     3841 accatgatta cgccaagctt gcatggcggc cgcataactt cgtatagcat acattatacg
     3901 aagttatgca tgctcggtgg gtcaggtgga agtggatctg gtgctatgat gggcagtgtc
     3961 gagctgaatc tgagggagac tgagctgtgt cttggtcttc ccggtggaga tacagtggct
     4021 ccggtaaccg gaaacaagag agggttctca gagacggttg atctgaagct aaatctgaat
     4081 aatgagcctg caaacaagga aggatctacg actcatgacg tcgtgacttt tgattccaag
     4141 gagaagagtg cttgtcctaa agatccagcc aaacctccgg ccaaggcaca agttgtggga
     4201 tggccaccgg tgagatcata ccggaagaac gtgatggttt cctgccaaaa atcaagcggt
     4261 ggcccggagg cggcggcgtt cgtgaaggta tcaatggacg gagcaccgta cttgaggaaa
     4321 atcgatttga ggatgtataa aagctacgat gagctttcta atgctttgtc caacatgttc
     4381 agctctttta ccatgggcaa acatggagga gaagaaggaa tgatagactt catgaatgag
     4441 aggaaattga tggatttggt gaatagctgg gactatgttc cctcttatga agacaaagac
     4501 ggtgattgga tgctcgtcgg cgacgttcct tggccaatgt tcgtcgatac atgcaagcgt
     4561 ttacgtctca tgaaaggatc ggatgccatt ggtctcgctc cgagggcgat ggagaagtgc
     4621 aagagcagag ctggtgctgg agcaggcgcc tacccatacg atgttcctga ctatgcgggc
     4681 tatccctatg acgtcccgga ctatgcagga tcctatccat atgacgttcc agattacgct
     4741 gctcagtgct gaggcgcgcc gctagcagtc ccggggcttg cccatctgtt ttagacgtca
     4801 cagaaggacc atgtttattt taaatttcac atacatgtgt ttaattacct tcattgtata
     4861 aattctcgaa actttctcca attcttcggt cagataaaaa atttctctag tcaccgtctt
     4921 ttatagagct tttgtcagat gtttttctgg atggaatcat tgtccagtta ctatgctttt
     4981 tatttataag tttgaaagtt tagttaactt tttgttttct ttaatacatt ttttaatcat
     5041 gtcaacattt gggatttgta gaagtagtaa attttttgta catgttgtag aattggataa
     5101 gaacgaaaac agctagtatg taaaaaaaac gaaaatccca aattccgttg tttgtttgat
     5161 atagtaatat ttatagcatt ttagtttcta aagatttttg ataatctttt acatttgctt
     5221 tcacatacat tcgcgctact accatacatc atatcccgct ttctttaatt gcttccccac
     5281 agactcaacc agcggggcct tcttctcgag atctgtttag cttgcctcgt ccccgccggg
     5341 tcacccggcc agcgacatgg aggcccagaa taccctcctt gacagtcttg acgtgcgcag
     5401 ctcaggggca tgatgtgact gtcgcccgta catttagccc atacatcccc atgtataatc
     5461 atttgcatcc atacattttg atggccgcac ggcgcgaagc aaaaattacg gctcctcgct
     5521 gcagacctgc gagcagggaa acgctcccct cacagacgcg ttgaattgtc cccacgccgc
     5581 gcccctgtag agaaatataa aaggttagga tttgccactg aggttcttct ttcatatact
     5641 tccttttaaa atcttgctag gatacagttc tcacatcaca tccgaacata aacaaccatg
     5701 ggtaaggaaa agactcacgt ttcgaggccg cgattaaatt ccaacatgga tgctgattta
     5761 tatgggtata aatgggctcg cgataatgtc gggcaatcag gtgcgacaat ctatcgattg
     5821 tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag cgttgccaat
     5881 gatgttacag atgagatggt cagactaaac tggctgacgg aatttatgcc tcttccgacc
     5941 atcaagcatt ttatccgtac tcctgatgat gcatggttac tcaccactgc gatccccggc
     6001 aaaacagcat tccaggtatt agaagaatat cctgattcag gtgaaaatat tgttgatgcg
     6061 ctggcagtgt tcctgcgccg gttgcattcg attcctgttt gtaattgtcc ttttaacagc
     6121 gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg
     6181 agtgattttg atgacgagcg taatggctgg cctgttgaac aagtctggaa agaaatgcat
     6241 aagcttttgc cattctcacc ggattcagtc gtcactcatg gtgatttctc acttgataac
     6301 cttatttttg acgaggggaa attaataggt tgtattgatg ttggacgagt cggaatcgca
     6361 gaccgatacc aggatcttgc catcctatgg aactgcctcg gtgagttttc tccttcatta
     6421 cagaaacggc tttttcaaaa atatggtatt gataatcctg atatgaataa attgcagttt
     6481 catttgatgc tcgatgagtt tttctaatca gtactgacaa taaaaagatt cttgttttca
     6541 agaacttgtc atttgtatag tttttttata ttgtagttgt tctattttaa tcaaatgtta
     6601 gcgtgattta tatttttttt cgcctcgaca tcatctgccc agatgcgaag ttaagtgcgc
     6661 agaaagtaat atcatgcgtc aatcgtatgt gaatgctggt cgctatactg ctgtcgattc
     6721 gatactaacg ccgccatcca gtcgacgaac tagtataact tcgtatatgg tattatatac
     6781 gaagttatgc ggccgcgcga attcactggc cgtcgtttta caacgtcgtg actgggaaaa
     6841 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     6901 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     6961 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcataccatc
     7021 gtccggatca atgaagtaga tgagtttact acctgtatat actttttttt ttgtcaaagt
     7081 cacttttatg gcatttccat gttgaaggat tagccacagc actaaatttt cagcgtatga
     7141 ttgcttttaa atatttaatt ttcatcgttt tttaattaat attcaaaacg atttaatgcc
     7201 ttctacatct gaagtcaagc tcatagactc gggttgggta gctaaaacta catctaacac
     7261 gaaggggtat agccttctta aagtatactc atgtataaac gttttgggtt catatttttg
     7321 ggccacgtgg tttataaaaa ttcttaacta caccactcgg tatcccgcac ccgtctacgt
     7381 ttctacgatt tcgagctaat attgagtaaa gtgattagca aaaaaaataa aagtactcgt
     7441 tgtcggagat caagaatttt tctattatct catctaaacc actttctaaa agcgaaaaac
     7501 aaaatcgtaa tatgcagctt gaatgggctt ccatagtttg aaagaaaaac cctagcagta
     7561 ctggcaaggg agacattcct tttacctggc taatcgccat cttccagcag gcgcaccatt
     7621 gcccctgttt cactatccag gttacggata tagttcatga caatatttac attggtccag
     7681 ccaccagctt gcatgatctc cggtattgaa actccagcgc gggccatatc tcgcgcggct
     7741 ccgacacggg cactgtgtcc agaccaggcc aggtatctct gaccagagtc atccttagcg
     7801 ccgtaaatca atcgatgagt tgcttcaaaa atcccttcca gggcgcgagt tgatagctgg
     7861 ctggtggcag atggcgcggc aacaccattt tttctgaccc ggcaaaacag gtagttattc
     7921 ggatcatcag ctacaccaga gacggaaatc catcgctcga ccagtttagt tacccccagg
     7981 ctaagtgcct tctctacacc tgcggtgcta accagcgttt tcgttctgcc aatatggatt
     8041 aacattctcc caccgtcagt acgtgagata tctttaaccc tgatcctggc aatttcggct
     8101 atacgtaaca gggtgttata agcaatcccc agaaatgcca gattacgtat atcctggcag
     8161 cgatcgctat tttccatgag tgaacgaacc tggtcgaaat cagtgcgttc gaacgctaga
     8221 gcctgttttg cacgttcacc ggcatcaacg ttttcttttc ggatccgccg cataaccagt
     8281 gaaacagcat tgctgtcact tggtcgtggc agcccggacc gacgatgaag catgtttagc
     8341 tggcccaaat gttgctggat agtttttact gccagaccgc gcgcctgaag atatagaaga
     8401 taatcgcgaa catcttcagg ttctgcggga aaccatttcc ggttattcaa cttgcaccat
     8461 gccgcccacg accggcaaac ggacagaagc attttccagg tatgctcaga aaacgcctgg
     8521 cgatccctga acatgtccat caggttcttg cgaacctcat cactcgttgc atcgaccggt
     8581 aacttttaga ttaacatttg tttaaatatg aaattaggca atgtaacata ctgcaggcaa
     8641 attttggtgt acggtaagta aattggacat atgatttaac aaagcgacta taagtcagaa
     8701 agtgagaatg agattgaaat aattaattca acttattcaa ttgatgatat gccaggattc
     8761 ctcttccttt atcttcaatg tttcgattaa caggtccatt atccggtttt tagtttcgct
     8821 gcacattgcc gaatgacaat cggattcctt taagctttgc ttttgccctg ttttctctcc
     8881 ccagtaatcg ttgggtgact gaaccatcca gtagccgaga gttacgtttt tcctgattta
     8941 ataaatatcc atatctattt gtctcgtggt gttttacagc aactaaccga aattactatt
     9001 ttgcatcatc caaccataaa gagacaacaa taattctaga aaacatataa caagtgtttt
     9061 tttctattta cgtctcatac gcaaagtcca ctttaccatg ttttggaaac ttgtacccaa
     9121 ccattcattt ttcgattttg taatgaggct cttttcttct aacaatcatt gaggtcatta
     9181 tcatgtgtcc tcacctattg tttaaattga ggcttgcgct attcccaaaa ttgtgggtat
     9241 tattttcata tatcagtatc tgcaatttcg gttcaactcc ggaagctcgg tacccaactg
     9301 tgggaatact caggtatcgt aagatgcaag agttcgaatc tcttagcaac cattattttt
     9361 tttctctcaa cataacgaga acacacaggg gcgctatcgc acagaatcaa attcgatgac
     9421 tggaaatttt ttgttaattt cagaggtcgc ctgacgcata tacctttttc aactgaaaaa
     9481 ttgggagaaa aaggaaaggt gagagcgccg gaaccggctt ttcatataga atagagaagc
     9541 gttcatgact aaatgcttgc atcacaatac ttgaagttga caatattatt taaggaccta
     9601 ttgttttttc caataggtgg ttagcaatcg tcttactttc taacttttct taccttttac
     9661 atttcagcaa tatatatata tatatatata tatatttcaa ggatatacca ttgtaatgtc
     9721 tgcccctaag aagatcgtcg ttttgccagg tgaccacgtt ggtcaagaaa tcacagccga
     9781 agccattaag gttcttaaag ctatttctga tgttcgttcc aatgtcaagt tcgatttcga
     9841 aaatcattta attggtggtg ctgctatcga tgctacaggt gttccacttc cagatgaggc
     9901 gctggaagcc tccaagaagg ctgatgccgt tttgttaggt gctgtgggtg gtcctaaatg
     9961 gggtaccggt agtgttagac ctgaacaagg tttactaaaa atccgtaaag aacttcaatt
    10021 gtacgccaac ttaagaccat gtaactttgc atccgactct cttttagact tatctccaat
    10081 caagccacaa tttgctaaag gtactgactt cgttgttgtt agagaattag tgggaggtat
    10141 ttactttggt aagagaaagg aagacgatgg tgatggtgtc gcttgggata gtgaacaata
    10201 caccgttcca gaagtgcaaa gaatcacaag aatggccgct ttcatggccc tacaacatga
    10261 gccaccattg cctatttggt ccttggataa agctaatgtt ttggcctctt caagattatg
    10321 gagaaaaact gtggaggaaa ccatcaagaa cgaatttcct acattgaaag ttcaacatca
    10381 attgattgat tctgccgcca tgatcctagt taagaaccca acccacctaa atggtattat
    10441 aatcaccagc aacatgtttg gtgatatcat ctccgatgaa gcctccgtta tcccaggctc
    10501 cttgggtttg ttgccatctg cgtccttggc ctctttgcca gacaagaaca ccgcatttgg
    10561 tttgtacgaa ccatgccatg gttccgctcc agatttgcca aagaataagg tcaaccctat
    10621 cgccactatc ttgtctgctg caatgatgtt gaaattgtca ttgaacttgc ctgaagaagg
    10681 taaagccatt gaagatgcag ttaaaaaggt tttggatgca ggtatcagaa ctggtgattt
    10741 aggtggttcc aacagtacca ccgaagtcgg tgatgctgtc gccgaagaag ttaagaaaat
    10801 ccttgcttaa aaagattctc tttttttgtg atatttgtac ataaacttta taaatgaaat
    10861 tcataataga aacgacacga aattacaaaa tggaatatgt tcatagggta gacgaaacta
    10921 tatacgcaat ctacatacat ttatcaagaa ggagaaaaag gaggatgtaa aggaatacag
    10981 gtaagcaaat tgatactaat ggctcaacgt gataaggaaa aagaattgca ctttaacatt
    11041 aatattgaca aggaggaggg catcaca
//
