LOCUS       sequence-33322-e         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Kim JH, Wei JR, Wallach JB, Robbins RS, Rubin EJ, Schnappinger D
  TITLE     Protein inactivation in mycobacteria by controlled proteolysis and 
            its application to deplete the beta subunit of RNA polymerase.
  JOURNAL   Nucleic Acids Res. 2011 Mar;39(6):2210-20. doi: 10.1093/nar/gkq1149
  PUBMED    21075796
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2011-03"; volume: "39"; issue: "6"; 
            pages: "2210-20"
COMMENT     Sequence Label: pGMEH-P38-luxABct6
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     545..567
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     559..576
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     560..576
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    584..604
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 tccgagtttg tcagaaagca gaccaaacag cggttggaat aatagcgaga acagagaaat
       61 agcggcaaaa ataatacccg tatcactttt gctgatatgg ttgatgtcat gtagccaaat
      121 cgggaaaaac gggaagtagg ctcccatgat aaaaaagtaa aagaaaaaga ataaaccgaa
      181 catccaaaag tttgtgtttt ttaaatagta cataatggat ttccttacgc gaaatacggg
      241 cagacatggc ctgcccggtt attattattt ttgacaccag accaactggt aatggtagcg
      301 accggcgctc agctggaatt ccgccgatac tgacgggctc caggagtcgt cgccaccaat
      361 ccccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc
      421 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
      481 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
      541 ttttcccagt cacgacgttg taaaacgacg gccagtgaat tatcaactat gtataataaa
      601 gttgaagctt gatatcgaat tcctgcagcc cgggggatcc atttaaatca gagctttcgc
      661 gagctcgaga tccggctgtt tttgttcctg caatgaccat tgctgaggag ttcccatgaa
      721 aatcaagtca tcgatttctg tgctcgctgc gatattttcc tgcttaaccg ctgaaagtac
      781 gtccatataa atgcctctat tagttagcgc tatcgcgcga aaagaatggt gatata
//
