LOCUS       sequence-33323-e         964 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 964)
  AUTHORS   Kim JH, Wei JR, Wallach JB, Robbins RS, Rubin EJ, Schnappinger D
  TITLE     Protein inactivation in mycobacteria by controlled proteolysis and 
            its application to deplete the beta subunit of RNA polymerase.
  JOURNAL   Nucleic Acids Res. 2011 Mar;39(6):2210-20. doi: 10.1093/nar/gkq1149
  PUBMED    21075796
REFERENCE   2  (bases 1 to 964)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2011-03"; volume: "39"; issue: "6"; 
            pages: "2210-20"
COMMENT     Sequence Label: pGMEH-P38-luxAFB-DAS+4
FEATURES             Location/Qualifiers
     source          1..964
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    9..33
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(525..549)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 agttgaattc aagtttgtac aaaaaagcag gcttattgga tcacgccgcg agcgtgtcgg
       61 tgttcgaaga cctgggaatc cagcaggcgc ccaacagcac cgtctgctcc gtcttctcca
      121 gaaaggcgac tacgcggtcc tcctcgtcgg tgggtacgca gccgaatgcc cgcgggtcgc
      181 ccacccgcac cagttgcagc gtgacatcgg ctcgattgct tcggtggaag tccagcagtt
      241 gggccagatc cgcgagggcc ccccgtgaac atcgtcgtgg taaccagcgt gtcggctttg
      301 gctgtggccg tggtgcattc ggtggcgttt gcgatcaagg ggcgtcacgg cgaggacctg
      361 gtcgaccgct ggaaccgtga gggcatcggt gcacaccgcg ggatcagacc gtcgacgaac
      421 catttgaatc cgaccggtac ctcaacgagt tgacggccga tcacgtccag gttatgttcg
      481 atgacgatca ccgtattgcc cttgtcgacc aggccgttga tccgacccag ctttcttgta
      541 caaagtggga aggagatata catatgaaat ttggaaactt ccttctcact tatcagccac
      601 ctgagctatc tcagaccgaa gtgatgaagc gattggttaa tctgggcaaa gcgtctgaag
      661 gttgtggctt cgacaccgtt tggttgctag agcaccactt cactgaattt gggttgttag
      721 ggaatcctta tgttgctgcc gcacacctat taggtgcgac agaaacgctc aacgttggca
      781 ctgcagctat cgtattgccg actgcccatc cggttcgaca agcagaagac gtaaacctac
      841 tggatcaaat gtcaaaagga cgattccgtt ttggtatttg tcgcggtttg tacgataaag
      901 attttcgtgt ctttggtaca gacatggata acagccgagc cttaatggac tgttggtatg
      961 actg
//
