LOCUS       sequence-33325-e         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Kim JH, Wei JR, Wallach JB, Robbins RS, Rubin EJ, Schnappinger D
  TITLE     Protein inactivation in mycobacteria by controlled proteolysis and 
            its application to deplete the beta subunit of RNA polymerase.
  JOURNAL   Nucleic Acids Res. 2011 Mar;39(6):2210-20. doi: 10.1093/nar/gkq1149
  PUBMED    21075796
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2011-03"; volume: "39"; issue: "6"; 
            pages: "2210-20"
COMMENT     Sequence Label: pGMCtN-P38-luxAB2
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    8..32
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(524..548)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttgaaattca agtttgtaca aaaaagcagg cttattggat cacgccgcga gcgtgtcggt
       61 gttcgaagac ctgggaatcc agcaggcgcc caacagcacc gtctgctccg tcttctccag
      121 aaaggcgact acgcggtcct cctcgtcggt gggtacgcag ccgaatgccc gcgggtcgcc
      181 cacccgcacc agttgcagcg tgacatcggc tcgattgctt cggtggaagt ccagcagttg
      241 ggccagatcc gcgagggccc cccgtgaaca tcgtcgtggt aaccagcgtg tcggctttgg
      301 ctgtggccgt ggtgcattcg gtggcgtttg cgatcaaggg gcgtcacggc gaggacctgg
      361 tcgaccgctg gaaccgtgag ggcatcggtg cacaccgcgg gatcagaccg tcgacgaacc
      421 atttgaatcc gaccggtacc tcaacgagtt gacggccgat cacgtccagg ttatgttcga
      481 tgacgatcac cgtattgccc ttgtcgacca ggccgttgat ccgacccagc tttcttgtac
      541 aaagtggtta attaacagga tccccaaata aggaaatgtt atgaaatttg gaaacttcct
      601 tctcacttat cagccacctg agctatctca gaccgaagtg atgaagcgat tggttaatct
      661 gggcaaagcg tctgaaggtt gtggcttcga caccgtttgg ttgctagagc accacttcac
      721 tgaatttggg ttgttaggga atccttatgt tgctgccgca cacctattag gtgcgacaga
      781 aacgctcaac gttggcactg cagctatcgt attgccgact gcccatccgg ttcgacaagc
      841 agaagacgta aacctactgg atcaaatgtc aaaa
//
