LOCUS       sequence-33327-e         983 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Kim JH, Wei JR, Wallach JB, Robbins RS, Rubin EJ, Schnappinger D
  TITLE     Protein inactivation in mycobacteria by controlled proteolysis and 
            its application to deplete the beta subunit of RNA polymerase.
  JOURNAL   Nucleic Acids Res. 2011 Mar;39(6):2210-20. doi: 10.1093/nar/gkq1149
  PUBMED    21075796
REFERENCE   2  (bases 1 to 983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2011-03"; volume: "39"; issue: "6"; 
            pages: "2210-20"
COMMENT     Sequence Label: pGMCtN-P38-luxAB-DAS+4
FEATURES             Location/Qualifiers
     source          1..983
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    39..59
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 ttaataaaag gggactctag ggtcccctgc agcttaagca actatgtata ataaagttga
       61 agcttgatat cgaattcctg cagcccgggg gatccattta aatcagagct ttcgcgagct
      121 cgagatccgg ctgtttttgt tcctgcaatg accattgctg aggagttccc atgaaaatca
      181 agtcatcgat ttctgtgctc gctgcgatat tttcctgctt aaccgctgaa agtacgtcca
      241 tataaatgcc tctattagtt agcgctatcg cgcgaaaaga atggtgatat aaggggcatc
      301 gctgcccctt cagcatcagt taaacgacta gttattagct ggcgtccgcg tagttctcgg
      361 agtagttctc gtcgttggcg gccgagtggt atttgacgat gttggcgttt accacatcga
      421 taaccgctct ttgctgcgct ttatcttcca tcgactcaaa agacatcaat aggtccgcgg
      481 caccacaaca ctcaatcgca actcgcgctg cctgagtact ttcttcataa gtaccgatgg
      541 cattttctga caacagctct cccatttttt gctcaaagtc ggtatttgag taagattcac
      601 ggacaaactc ttccaaatac acgcgagctt ctgcccttgc tgcttcacca tctacatttt
      661 ggttgaccag cagcgtcagc ttgtgtcgaa cctgactaac atcgacacca tgtgcctgag
      721 caacttcgtg gtacaaaccg gcgtattctt ttctttgagc gtttgagtcg tcccatctaa
      781 acacgagtgg aagccctaac ttagccgccc attcaaccac ttctttgctc gtcgcattca
      841 caaattgcgc aggancgcct tcagtgaacg cgtgtgggtt aacggagatt ttaggaaaac
      901 tataaaaatc attgtttgga tggcagtacc cagtagtgaa tgcatcattg atgatctggt
      961 gacactcact gaacaactga aac
//
