LOCUS       sequence-333272-        4325 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutively expressed , mutationally-enhanced chimeric 
            transcription activator comprising the AraC DNA binding domain and 
            the XylS benzoate ligand binding domain..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4325)
  AUTHORS   Picard HR, Schwingen KS, Green LM, Shis DL, Egan SM, Bennett MR, 
            Swint-Kruse L
  TITLE     Allosteric regulation within the highly interconnected structural 
            scaffold of AraC/XylS homologs tolerates a wide range of amino acid 
            changes.
  JOURNAL   Proteins. 2021 Aug 8. doi: 10.1002/prot.26206.
  PUBMED    34369028
REFERENCE   2  (bases 1 to 4325)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proteins. 
            2021 Aug 8. doi: 10.1002/prot.26206."
COMMENT     Sequence Label: XXXXA_Q182A_R287H-pHG165c
FEATURES             Location/Qualifiers
     source          1..4325
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(19..41)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    127..267
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     182..201
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(282..299)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(453..1041)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(533..552)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1212..2003,2004..2072))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1835..1854
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2073..2177)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2245..2263
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     2416..2438
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2430..2447
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2431..2447
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             join(4159..4325,1..25)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct
       61 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg
      121 tcggggcgca gtcatgaccc agtcacgtag cgatagcgga gtgtatactg gcttaactat
      181 gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga
      241 tgcgtaagga gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg
      301 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
      361 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
      421 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
      481 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
      541 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
      601 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
      661 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
      721 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
      781 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
      841 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
      901 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
      961 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     1021 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     1081 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     1141 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     1201 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     1261 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     1321 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     1381 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     1441 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     1501 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     1561 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     1621 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     1681 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     1741 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     1801 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     1861 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     1921 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     1981 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     2041 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     2101 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     2161 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
     2221 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tcttcaagaa
     2281 tccgttaacg gcattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
     2341 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
     2401 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagcttggct
     2461 gcaggtcgac ggatccccgg gaattccacc atcgaatggt gacaccatcg aatggtgcaa
     2521 aacctttcgc ggtatggcat gatagcgccc ggggcccaag ttcacttaaa aaggagatca
     2581 acaatgaagc aattttcgta ctgaaacatc ttaatcatgc tggggagggt ttctaatgga
     2641 tttttgcctg ctgaacgaaa aaagccagat ttttgtgcat gcggaaccgt atgcggtgag
     2701 cgattatgtg aaccagtatg tgggcaccca tagcattcgc ctgccgaaag gcggccgccc
     2761 ggcgggccgc ctgcatcatc gcatttttgg ctgcctggat ctgtgccgca ttagctatgg
     2821 cggcagcgtg cgcgtgatta gcccgggcct ggaaacctgc tatcatctgc agattattct
     2881 gaaaggccat tgcctgtggc gcggccatgg ccaggaacat tattttgcgc cgggcgaact
     2941 gctgctgctg aacccggatg atcaggcgga tctgacctat agcgaagatt gcgaaaaatt
     3001 tattgtgaaa ctgccgagcg tggtgctgga tcgcgcgtgc agcgataaca actggcataa
     3061 accgcgcgaa ggcattcgct ttgcggcgcg ccataacctg cagcagctgg atggctttat
     3121 taacctgctg ggcctggtgt gcgatgaagc ggaacatacc aaaagcatgc cgcgcgtggc
     3181 tgaacattat gcgggcatta ttgcgagcaa actgctggaa atgctgggca gcaacgtgag
     3241 ccgcgaaatt tttagcaaag gcaacccgga taatcgggta cgcgaggctt gtcagtacat
     3301 cagcgatcac ctggcagaca gcaattttga tatcgccagc gtcgcacagc atgtttgctt
     3361 gtcgccgtcg cgtctgtcac atcttttccg ccagcagtta gggattagcg tcttaagctg
     3421 gcgcgaggac caacgtatca gccaggcgaa gctgcttttg agcaccaccc ggatgcctat
     3481 cgccaccgtc ggtcacaatg ttggttttga cgatcaactc tatttctcgc gggtatttaa
     3541 aaaatgcacc ggggccagcc cgagcgagtt ccgtgccggt tgtgaagaaa aagtgaatga
     3601 tgtagccgtc aagttgtcat aatgagaatt cgtaataata tagctctaga ttaattgcgc
     3661 ccgttaacgg cgggcagcgt tgggtcctgg ccacgggtgc gcatgatcgt gctcctgtcg
     3721 ttgaggaccc ggctaggctg gcggggttgc cttactggtt agcagaatga atcaccgata
     3781 cgcgagcgaa cgtgaagcga ctgctgctgc aaaacgtctg cgacctgagc aacaacatga
     3841 atggtcttcg gtttccgtgt ttcgtaaagt ctggaaacgc ggaagtcagc gccctgcacc
     3901 attatgttcc ggatctgcat cgcaggatgc tgctggctac cctgtggaac acctacatct
     3961 gtattaacga agcgctggca ttgaccctga gtgatttttc tctggtcccg ccgcatccat
     4021 accgccagtt gtttaccctc acaacgttcc agtaaccggg catgttcatc atcagtaacc
     4081 cgtatcgtga gcatcctctc tcgtttcatc ggtatcatta cccccatgaa cagaaatccc
     4141 ccttacacgg aggcatcagt gaccaaacag gaaaaaaccg cccttaacat ggcccgcttt
     4201 atcagaagcc agacattaac gcttctggag aaactcaacg agctggacgc ggatgaacag
     4261 gcagacatct gtgaatcgct tcacgaccac gctgatgagc tttaccgcag ctgcctcgcg
     4321 cgttt
//
