LOCUS       sequence-333273-        4214 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutively expressed, chimeric transcription activator 
            comprising the AraC DNA binding domain and the RhaR rhamnose ligand 
            binding domains..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4214)
  AUTHORS   Picard HR, Schwingen KS, Green LM, Shis DL, Egan SM, Bennett MR, 
            Swint-Kruse L
  TITLE     Allosteric regulation within the highly interconnected structural 
            scaffold of AraC/XylS homologs tolerates a wide range of amino acid 
            changes.
  JOURNAL   Proteins. 2021 Aug 8. doi: 10.1002/prot.26206.
  PUBMED    34369028
REFERENCE   2  (bases 1 to 4214)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proteins. 
            2021 Aug 8. doi: 10.1002/prot.26206."
COMMENT     Sequence Label: RRRAA-pHG165c
FEATURES             Location/Qualifiers
     source          1..4214
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..65
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(146..163)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(317..905)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(397..416)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1076..1867,1868..1936))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1699..1718
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1937..2041)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2109..2127
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     2280..2302
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2294..2311
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2295..2311
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             3912..4103
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(4097..4119)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    join(4205..4214,1..131)
                     /label=bom
                     /note="basis of mobility region from pBR322"
ORIGIN
        1 acccagtcac gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga
       61 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
      121 accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
      181 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
      241 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
      301 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
      361 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
      421 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
      481 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
      541 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
      601 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
      661 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
      721 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
      781 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
      841 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
      901 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
      961 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     1021 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     1081 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     1141 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     1201 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     1261 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     1321 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     1381 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     1441 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     1501 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     1561 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     1621 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     1681 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     1741 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     1801 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     1861 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     1921 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     1981 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     2041 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
     2101 cctataaaaa taggcgtatc acgaggccct ttcgtcttca agaatccgtt aacggcattc
     2161 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
     2221 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
     2281 ccagtcacga cgttgtaaaa cgacggccag tgccaagctt ggctgcaggt cgacggatcc
     2341 ccgggaattc caccatcgaa tggtgacacc atcgaatggt gcaaaacctt tcgcggtatg
     2401 gcatgatagc gcccggggcc caagttcact taaaaaggag atcaacaatg aagcaatttt
     2461 cgtactgaaa catcttaatc atgctgggga gggtttctaa tggcgcatca gttaaaactt
     2521 ctcaaagatg atttttttgc cagcgaccag caggcagtcg ctgtggctga ccgttatccg
     2581 caagatgtct ttgctgaaca tacacatgat ttttgtgagc tggtgattgt ctggcgcggt
     2641 aatggcctgc atgtactcaa cgatcgccct tatcgcatta cccgtggcga tctcttttac
     2701 attcatgctg acgataaaca ctcctacgct tccgttaacg atctggtttt gcagaatatt
     2761 atttattgcc cggagcgtct gaagctgaat cttgactggg tgggggcgat tccgggattt
     2821 aacgccagcg cagggcaacc acactggcgc ttaggtagca tggggatggc gcaggcgcgg
     2881 caggttatcg gtcagcttga gcatgaaagt agtcagcatg tgccgtttgc taacgaaatg
     2941 gctgagttgc tgttcgggca gttggtgatg ttgctgaatc gccatcgtta caccagtgat
     3001 tcgttgccgc caacatccag cgataatcgg gtacgcgagg cttgtcagta catcagcgat
     3061 cacctggcag acagcaattt tgatatcgcc agcgtcgcac agcatgtttg cttgtcgccg
     3121 tcgcgtctgt cacatctttt ccgccagcag ttagggatta gcgtcttaag ctggcgcgag
     3181 gaccaacgta tcagccaggc gaagctgctt ttgagcacca cccggatgcc tatcgccacc
     3241 gtcggtcgca atgttggttt tgacgatcaa ctctatttct cgcgggtatt taaaaaatgc
     3301 accggggcca gcccgagcga gttccgtgcc ggttgtgaag aaaaagtgaa tgatgtagcc
     3361 gtcaagttgt cataatgaga attcgtaata atatagctct agattaattg cgcccgttaa
     3421 cggcgggcag cgttgggtcc tggccacggg tgcgcatgat cgtgctcctg tcgttgagga
     3481 cccggctagg ctggcggggt tgccttactg gttagcagaa tgaatcaccg atacgcgagc
     3541 gaacgtgaag cgactgctgc tgcaaaacgt ctgcgacctg agcaacaaca tgaatggtct
     3601 tcggtttccg tgtttcgtaa agtctggaaa cgcggaagtc agcgccctgc accattatgt
     3661 tccggatctg catcgcagga tgctgctggc taccctgtgg aacacctaca tctgtattaa
     3721 cgaagcgctg gcattgaccc tgagtgattt ttctctggtc ccgccgcatc cataccgcca
     3781 gttgtttacc ctcacaacgt tccagtaacc gggcatgttc atcatcagta acccgtatcg
     3841 tgagcatcct ctctcgtttc atcggtatca ttacccccat gaacagaaat cccccttaca
     3901 cggaggcatc agtgaccaaa caggaaaaaa ccgcccttaa catggcccgc tttatcagaa
     3961 gccagacatt aacgcttctg gagaaactca acgagctgga cgcggatgaa caggcagaca
     4021 tctgtgaatc gcttcacgac cacgctgatg agctttaccg cagctgcctc gcgcgtttcg
     4081 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
     4141 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
     4201 ggggcgcagc catg
//
