LOCUS       sequence-333275-        4208 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutively expressed, mutationally-enhanced chimeric 
            transcription activator comprising the AraC DNA binding domain and 
            the RhaR rhamnose ligand binding domains..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4208)
  AUTHORS   Picard HR, Schwingen KS, Green LM, Shis DL, Egan SM, Bennett MR, 
            Swint-Kruse L
  TITLE     Allosteric regulation within the highly interconnected structural 
            scaffold of AraC/XylS homologs tolerates a wide range of amino acid 
            changes.
  JOURNAL   Proteins. 2021 Aug 8. doi: 10.1002/prot.26206.
  PUBMED    34369028
REFERENCE   2  (bases 1 to 4208)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proteins. 
            2021 Aug 8. doi: 10.1002/prot.26206."
COMMENT     Sequence Label: RRRAA-5-mut-pHG165c
FEATURES             Location/Qualifiers
     source          1..4208
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    8..148
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     63..82
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(163..180)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(334..922)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(414..433)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1093..1884,1885..1953))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1716..1735
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1954..2058)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2126..2144
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     2297..2319
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2311..2328
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2312..2328
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             3923..4114
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(4108..4130)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta
       61 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
      121 atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct
      181 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
      241 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
      301 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
      361 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
      421 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
      481 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
      541 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
      601 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
      661 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
      721 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
      781 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
      841 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
      901 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
      961 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     1021 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     1081 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     1141 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     1201 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     1261 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     1321 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     1381 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     1441 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     1501 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     1561 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     1621 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     1681 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     1741 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     1801 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     1861 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     1921 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     1981 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     2041 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat
     2101 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcaaga
     2161 atccgttaac ggcattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
     2221 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg
     2281 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtgc caagcttggc
     2341 tgcaggtcga cggatccccg ggaattccac catcgaatgg tgacaccatc gaatggtgca
     2401 aaacctttcg cggtatggca tgatagcgcc cggggcccaa gttcacttaa aaaggagatc
     2461 aacaatgaag caattttcgt actgaaacat cttaatcatg ctggggaggg tttctaatgg
     2521 cgcatcagtt aaaacttctc aaagatgatt tttttctcag cgaccagcag gcagtcgctg
     2581 tggctgaccg ttatccgcaa gatgtctttg ctgaacatac acatgatttt tgtgagctgg
     2641 tgattgtctg gcgcggtaat ggcctgcatg tactcaacga tcgcccttat cgcattaccc
     2701 gtggcgatct cttttacatt catgctgacg ataaacactc ctacgcttcc gttaacgatc
     2761 tggttttgca gaatattatt tattgcccgg agcgtctgaa gctgaatctt gactggcagg
     2821 gggcgattcc gggatttaac gccagcgcag ggcaaccaca ccaacgccta ggtagcatgg
     2881 ggatggcgca ggcgcggcag gttatcggtc agcttgagca tgaaagtagt cagcatgtgc
     2941 cgtttgctaa cgaaatggct gagttgctgt tcgggcagtt ggtgatgttg ttgaatcgcc
     3001 atcgttacaa cgagtcgctc catccaccga tggataatcg ggtacgcaag gcctgtcagt
     3061 acatcacgga tcacctggca gatagcaatt ttgatatcgc cagcgtcgca cagcatgttt
     3121 gcttgtcgcc gtcgcgtctg tcacatcttt tccgccagca gttagggatt agcgtcttaa
     3181 gctggcgcga ggaccaacgt atcagccagg ctaagctgct tttgttaacc acccggatgc
     3241 ctatcgccac cgtcggtcgc aatgttggtt ttgacgatca actctatttc tcgcgggtat
     3301 ttaaaaaatg caccggggcc agcccgagcg agttccgtgc cggttgtgaa gaaaaagtga
     3361 atgatgtagc cgtcaagttg tcataatgag aattcgtaat aatatagctc tagattaatt
     3421 gcgcccgtta acggcgggca gcgttgggtc ctggccacgg gtgcgcatga tcgtgctcct
     3481 gtcgttgagg acccggctag gctggcgggg ttgccttact ggttagcaga atgaatcacc
     3541 gatacgcgag cgaacgtgaa gcgactgctg ctgcaaaacg tctgcgacct gagcaacaac
     3601 atgaatggtc ttcggtttcc gtgtttcgta aagtctggaa acgcggaagt cagcgccctg
     3661 caccattatg ttccggatct gcatcgcagg atgctgctgg ctaccctgtg gaacacctac
     3721 atctgtatta acgaagcgct ggcattgacc ctgagtgatt tttctctggt cccgccgcat
     3781 ccataccgcc agttgtttac cctcacaacg ttccagtaac cgggcatgtt catcatcagt
     3841 aacccgtatc gtgagcatcc tctctcgttt catcggtatc attaccccca tgaacagaaa
     3901 tcccccttac acggaggcat cagtgaccaa acaggaaaaa accgccctta acatggcccg
     3961 ctttatcaga agccagacat taacgcttct ggagaaactc aacgagctgg acgcggatga
     4021 acaggcagac atctgtgaat cgcttcacga ccacgctgat gagctttacc gcagctgcct
     4081 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
     4141 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
     4201 tggcgggt
//
