LOCUS       sequence-333277-        4217 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutively expressed, wild-type E coli AraC transcription 
            activator; activated by L-Arabinose..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4217)
  AUTHORS   Picard HR, Schwingen KS, Green LM, Shis DL, Egan SM, Bennett MR, 
            Swint-Kruse L
  TITLE     Allosteric regulation within the highly interconnected structural 
            scaffold of AraC/XylS homologs tolerates a wide range of amino acid 
            changes.
  JOURNAL   Proteins. 2021 Aug 8. doi: 10.1002/prot.26206.
  PUBMED    34369028
REFERENCE   2  (bases 1 to 4217)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proteins. 
            2021 Aug 8. doi: 10.1002/prot.26206."
COMMENT     Sequence Label: AraC-pHG165c WT
FEATURES             Location/Qualifiers
     source          1..4217
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     77..94
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    109..249
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(175..194)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     335..357
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(351..542)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(1079..1957)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     primer_bind     complement(2146..2162)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2155..2177)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(2330..2348)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2416..2520
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2521..2589,2590..3381)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2739..2758)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3552..4140
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4041..4060
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
       61 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta
      121 ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat
      181 ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc
      241 atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
      301 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
      361 tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga
      421 agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc
      481 gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc
      541 actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga
      601 gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt
      661 gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa
      721 tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg
      781 atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa
      841 acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag
      901 tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc
      961 cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca
     1021 acgctgcccg ccgttaacgg gcgcaattaa tctagagcta tattattacg aattctcatt
     1081 atgacaactt gacggctaca tcattcactt tttcttcaca accggcacgg aactcgctcg
     1141 ggctggcccc ggtgcatttt ttaaataccc gcgagaaata gagttgatcg tcaaaaccaa
     1201 cattgcgacc gacggtggcg ataggcatcc gggtggtgct caaaagcagc ttcgcctggc
     1261 tgatacgttg gtcctcgcgc cagcttaaga cgctaatccc taactgctgg cggaaaagat
     1321 gtgacagacg cgacggcgac aagcaaacat gctgtgcgac gctggcgata tcaaaattgc
     1381 tgtctgccag gtgatcgctg atgtactgac aagcctcgcg tacccgatta tccatcggtg
     1441 gatggagcga ctcgttaatc gcttccatgc gccgcagtaa caattgctca agcagattta
     1501 tcgccagcag ctccgaatag cgcccttccc cttgcccggc gttaatgatt tgcccaaaca
     1561 ggtcgctgaa atgcggctgg tgcgcttcat ccgggcgaaa gaaccccgta ttggcaaata
     1621 ttgacggcca gttaagccat tcatgccagt aggcgcgcgg acgaaagtaa acccactggt
     1681 gataccattc gcgagcctcc ggatgacgac cgtagtgatg aatctctcct ggcgggaaca
     1741 gcaaaatatc acccggtcgg caaacaaatt ctcgtccctg atttttcacc accccctgac
     1801 cgcgaatggt gagattgaga atataacctt tcattcccag cggtcggtcg ataaaaaaat
     1861 cgagataacc gttggcctca atcggcgtta aacccgccac cagatgggca ttaaacgagt
     1921 atcccggcag caggggatca ttttgcgctt cagccattag aaaccctccc cagcatgatt
     1981 aagatgtttc agtacgaaaa ttgcttcatt gttgatctcc tttttaagtg aacttgggcc
     2041 ccgggcgcta tcatgccata ccgcgaaagg ttttgcacca ttcgatggtg tcaccattcg
     2101 atggtggaat tcccggggat ccgtcgacct gcagccaagc ttggcactgg ccgtcgtttt
     2161 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
     2221 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
     2281 gcgcagcctg aatggcgaat gccgttaacg gattcttgaa gacgaaaggg cctcgtgata
     2341 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     2401 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     2461 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     2521 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     2581 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     2641 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     2701 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     2761 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     2821 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     2881 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     2941 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     3001 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     3061 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     3121 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     3181 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     3241 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     3301 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     3361 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     3421 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     3481 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     3541 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     3601 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     3661 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     3721 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     3781 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     3841 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     3901 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     3961 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     4021 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     4081 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     4141 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     4201 ttctttcctg cgttatc
//
