LOCUS       sequence-33328-e         947 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Kim JH, Wei JR, Wallach JB, Robbins RS, Rubin EJ, Schnappinger D
  TITLE     Protein inactivation in mycobacteria by controlled proteolysis and 
            its application to deplete the beta subunit of RNA polymerase.
  JOURNAL   Nucleic Acids Res. 2011 Mar;39(6):2210-20. doi: 10.1093/nar/gkq1149
  PUBMED    21075796
REFERENCE   2  (bases 1 to 947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2011-03"; volume: "39"; issue: "6"; 
            pages: "2210-20"
COMMENT     Sequence Label: pGMCKq1-0X1-sspBmyc
FEATURES             Location/Qualifiers
     source          1..947
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    6..26
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    30..54
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(443..467)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gctatcaact ttgtatagaa aagttgaatt caagtttgta caaaaaagca ggctaccagg
       61 cctagatctg gggaccctag aggtcccctt ttttatttta aaaatttttt cacgtgccag
      121 ccgattggac tagaaatatt ggatcgtcgg caccgtcacg gccgtgggag gcggcacgat
      181 ccgcgacgtg atgatcggcc gcatccccac ggtgctgcgc agtgagctct acgccatccc
      241 ggcgttgatc tgtgcgttcg cacgcacagg cccggtgtga gaagggtctc tgacgagcgg
      301 gagaactccc tatcagtgat agagtttgtc ctccctatca gtgatagata ggctctggga
      361 gtacccgtgt gtacgaccag cacggcatac atcatttcga cgccgagaga ttcgccgccc
      421 gaaatgagca cgatccgcat gcacccagct ttcttgtaca aagtgggctc gaggcatgcg
      481 aaggagatat acatatggac ctgtcgcagc tgaccccgcg ccggccctac ctgctgcgcg
      541 ccttctacga gtggttgctg gacaaccagc tgaccccgca cctggtggtg gacgtgaccc
      601 tgccgggcgt gcaggtgccg atggagtacg cgcgcgacgg ccagatcgtg ctgaacatcg
      661 cgccgcgcgc cgtcggcaac ctggagctgg cgaacgacga ggtgcgcttc aacgcgcgtt
      721 tcggtggcat cccgcggcag gtctcggtgc cgctggcggc cgtgctggcc atctacgccc
      781 gcgagaacgg cgccggcacc atgttcgagc cggaggcggc ctacgacgag gacacctcga
      841 tcatgaacga cgaggaggcc tcggccgaca acgagaccgt gatgtcggtg atcgacggcg
      901 acaagccgga ccacgacgat gacacccacc cggacgatga gccgccc
//
