LOCUS       sequence-33329-e         905 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Kim JH, Wei JR, Wallach JB, Robbins RS, Rubin EJ, Schnappinger D
  TITLE     Protein inactivation in mycobacteria by controlled proteolysis and 
            its application to deplete the beta subunit of RNA polymerase.
  JOURNAL   Nucleic Acids Res. 2011 Mar;39(6):2210-20. doi: 10.1093/nar/gkq1149
  PUBMED    21075796
REFERENCE   2  (bases 1 to 905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2011-03"; volume: "39"; issue: "6"; 
            pages: "2210-20"
COMMENT     Sequence Label: pGMCKq1-10M1-sspBmyc
FEATURES             Location/Qualifiers
     source          1..905
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    310..330
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    859..883
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
       61 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgtcgg
      121 ccagtgaaac gcgtagacgc tggttgaccg ttgcgggtac cggatccaaa gtcgctactc
      181 actggggccg ttgcttaagc ttgaagcggc tacgcaacat cggggaggag cggcgtgttg
      241 gacttcttac cggttataaa aaatttttaa aataaaaaag gggacctcta gggtcccctg
      301 cagcttaagc aactatgtat aataaagttg ctattacttc acgacgcgca gggccgggcg
      361 accgccgcgc ggcggctggg gcggctcatc gtccgggtgg gtgtcatcgt cgtggtccgg
      421 cttgtcgccg tcgatcaccg acatcacggt ctcgttgtcg gccgaggcct cctcgtcgtt
      481 catgatcgag gtgtcctcgt cgtaggccgc ctccggctcg aacatggtgc cggcgccgtt
      541 ctcgcgggcg tagatggcca gcacggccgc cagcggcacc gagacctgcc gcgggatgcc
      601 accgaagcgc gcgttgaagc gcacctcgtc gttcgccagc tccaggttgc cgacggcgcg
      661 cggcgcgatg ttcagcacga tctggccgtc gcgcgcgtac tccatcggca cctgcacgcc
      721 cggcagggtc acgtccacca ccaggtgcgg ggtcagctgg ttgtccagca accactcgta
      781 gaaggcgcgc agcaggtagg gccggcgcgg ggtcagctgc gacaggtcca tatgtatatc
      841 tccttcgcat gcctcgagcc cactttgtac aagaaagctg ggtgcatgcg gatcgtgctc
      901 atttc
//
