LOCUS       sequence-333326-       26002 bp    DNA     circular UNA 15-DEC-2025
DEFINITION  Vector for plant genome editing containing p35S:GFP-Cas9 expression 
            cassette, nptII selection marker, and SlFAST2 and Ca-Bs3 
            counterselction markers, but not sgRNAs..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 26002)
  AUTHORS   Stuttmann J, Barthel K, Martin P, Ordon J, Erickson JL, Herr R, 
            Ferik F, Kretschmer C, Berner T, Keilwagen J, Marillonnet S, Bonas U
  TITLE     Highly efficient multiplex editing: One-shot generation of 8x 
            Nicotiana benthamiana and 12x Arabidopsis mutants.
  JOURNAL   Plant J. 2021 Feb 12. doi: 10.1111/tpj.15197.
  PUBMED    33577114
REFERENCE   2  (bases 1 to 26002)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2021 Feb 12. doi: 10.1111/tpj.15197."
COMMENT     Sequence Label: pDGE687
FEATURES             Location/Qualifiers
     source          1..26002
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1062..1691
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2120..3193
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      3259..3453
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(3689..3711)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    3797..3937
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     3852..3871
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3952..3969)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4123..4711)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4203..4222)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(4957..5748)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASCADQLEEFVHYVKGEITKVVGK"
     misc_feature    6269..6293
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(6365..6617)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(6630..7424)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6741..6760)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     7351..7370
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     misc_feature    7426..7481
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     promoter        complement(7517..7696)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             complement(9703..10431)
                     /codon_start=1
                     /product="photostable variant of TagRFP (Shaner et al., 
                     2008)"
                     /label=TagRFP-T
                     /translation="VSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMR
                     IKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGV
                     LTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTDM
                     ALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAVA
                     RYCDLPSKLGHKLNGMDELYK"
     CDS             complement(10444..11175)
                     /codon_start=1
                     /product="photostable variant of TagRFP (Shaner et al., 
                     2008)"
                     /label=TagRFP-T
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
     terminator      complement(12253..12960)
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     CDS             complement(16209..16229)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(join(21959..22669,22670..22672))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(22001..22022)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     22350..22369
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     22609..22630
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(22685..22711)
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     promoter        complement(22808..23153)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(22845..22865)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     promoter        24177..24279
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             24280..24963
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGRKRR
                     VDPAY"
     primer_bind     complement(24346..24365)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             25283..25588
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     25467..25486
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     misc_feature    25709..25733
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ttcctggcgt tttcttgtcg cgtgttttag tcgcataaag tagaatactt gcgactagaa
       61 ccggagacat tacgccatga acaagagcgc cgccgctggc ctgctgggct atgcccgcgt
      121 cagcaccgac gaccaggact tgaccaacca acgggccgaa ctgcacgcgg ccggctgcac
      181 caagctgttt tccgagaaga tcaccggcac caggcgcgac cgcccggagc tggccaggat
      241 gcttgaccac ctacgccctg gcgacgttgt gacagtgacc aggctagacc gcctggcccg
      301 cagcacccgc gacctactgg acattgccga gagcatccag gaggccggcg cgggcctgcg
      361 tagcctggca gagccgtggg ccgacaccac cacgccggcc ggccgcatgg tgttgaccgt
      421 gttcgccggc attgccgagt tcgagcgttc cctaatcatc gaccgcaccc ggagcgggcg
      481 cgaggccgcc aaggcccgag gcgtgaagtt tggcccccgc cctaccctca ccccggcaca
      541 gatcgcgcac gcccgcgagc tgatcgacca ggaaggccgc accgtgaaag aggcggctgc
      601 actgcttggc gtgcatcgct cgaccctgta ccgcgcactt gagcgcagcg aggaagtgac
      661 gcccaccgag gccaggcggc gcggtgcctt ccgtgaggac gcattgaccg aggccgacgc
      721 cctggcggcc gccgagaatg aacgccaaga ggaacaagca tgaaaccgca ccaggacggc
      781 caggacgaac cgtttttcat taccgaagag atcgaggcgg agatgatcgc ggccgggtac
      841 gtgttcgagc cgcccgcgca cgtctcaacc gtgcggctgc atgaaatcct ggccggtttg
      901 tctgatgcca agctggcggc ctggccggcc agcttggccg ctgaagaaac cgagcgccgc
      961 cgtctaaaaa ggtgatgtgt atttgagtaa aacagcttgc gtcatgcggt cgctgcgtat
     1021 atgatgcgat gagtaaataa acaaatacgc aaggggaacg catgaaggtt atcgctgtac
     1081 ttaaccagaa aggcgggtca ggcaagacga ccatcgcaac ccatctagcc cgcgccctgc
     1141 aactcgccgg ggccgatgtt ctgttagtcg attccgatcc ccagggcagt gcccgcgatt
     1201 gggcggccgt gcgggaagat caaccgctaa ccgttgtcgg catcgaccgc ccgacgattg
     1261 accgcgacgt gaaggccatc ggccggcgcg acttcgtagt gatcgacgga gcgccccagg
     1321 cggcggactt ggctgtgtcc gcgatcaagg cagccgactt cgtgctgatt ccggtgcagc
     1381 caagccctta cgacatatgg gccaccgccg acctggtgga gctggttaag cagcgcattg
     1441 aggtcacgga tggaaggcta caagcggcct ttgtagtgtc gcgggcgatc aaaggcacgc
     1501 gcatcggcgg tgaggttgcc gaggcgctgg ccgggtacga gctgcccatt cttgagtccc
     1561 gtatcacgca gcgcgtgagc tacccaggca ctgccgccgc cggcacaacc gttcttgaat
     1621 cagaacccga gggcgacgct gcccgcgagg tccaggcgct ggccgctgaa attaaatcaa
     1681 aactcatttg agttaatgag gtaaagagaa aatgagcaaa agcacaaaca cgctaagtgc
     1741 cggccgtccg agcgcacgca gcagcaaggc tgcaacgttg gccagcctgg cagacacgcc
     1801 agccatgaag cgggtcaact ttcagttgcc ggcggaggat cacaccaagc tgaagatgta
     1861 cgcggtacgc caaggcaaga ccattaccga gctgctatct gaatacatcg cgcagctacc
     1921 agagtaaatg agcaaatgaa taaatgagta gatgaatttt agcggctaaa ggaggcggca
     1981 tggaaaatca agaacaacca ggcaccgacg ccgtggaatg ccccatgtgt ggaggaacgg
     2041 gcggttggcc aggcgtaagc ggctgggttg tctgccggcc ctgcaatggc actggaaccc
     2101 ccaagcccga ggaatcggcg tgagcggtcg caaaccatcc ggcccggtac aaatcggcgc
     2161 ggcgctgggt gatgacctgg tggagaagtt gaaggccgcg caggccgccc agcggcaacg
     2221 catcgaggca gaagcacgcc ccggtgaatc gtggcaagcg gccgctgatc gaatccgcaa
     2281 agaatcccgg caaccgccgg cagccggtgc gccgtcgatt aggaagccgc ccaagggcga
     2341 cgagcaacca gattttttcg ttccgatgct ctatgacgtg ggcacccgcg atagtcgcag
     2401 catcatggac gtggccgttt tccgtctgtc gaagcgtgac cgacgagctg gcgaggtgat
     2461 ccgctacgag cttccagacg ggcacgtaga ggtttccgca gggccggccg gcatggccag
     2521 tgtgtgggat tacgacctgg tactgatggc ggtttcccat ctaaccgaat ccatgaaccg
     2581 ataccgggaa gggaagggag acaagcccgg ccgcgtgttc cgtccacacg ttgcggacgt
     2641 actcaagttc tgccggcgag ccgatggcgg aaagcagaaa gacgacctgg tagaaacctg
     2701 cattcggtta aacaccacgc acgttgccat gcagcgtacg aagaaggcca agaacggccg
     2761 cctggtgacg gtatccgagg gtgaagcctt gattagccgc tacaagatcg taaagagcga
     2821 aaccgggcgg ccggagtaca tcgagatcga gctagctgat tggatgtacc gcgagatcac
     2881 agaaggcaag aacccggacg tgctgacggt tcaccccgat tactttttga tcgatcccgg
     2941 catcggccgt tttctctacc gcctggcacg ccgcgccgca ggcaaggcag aagccagatg
     3001 gttgttcaag acgatctacg aacgcagtgg cagcgccgga gagttcaaga agttctgttt
     3061 caccgtgcgc aagctgatcg ggtcaaatga cctgccggag tacgatttga aggaggaggc
     3121 ggggcaggct ggcccgatcc tagtcatgcg ctaccgcaac ctgatcgagg gcgaagcatc
     3181 cgccggttcc taatgtacgg agcagatgct agggcaaatt gccctagcag gggaaaaagg
     3241 tcgaaaaggt gtctttcctg tggatagcac gtacattggg aacccaaagc cgtacattgg
     3301 gaaccggaac ccgtacattg ggaacccaaa gccgtacatt gggaaccggt cacacatgta
     3361 agtgactgat ataaaagaga aaaaaggcga tttttccgcc taaaactctt taaaacttat
     3421 taaaactctt aaaacccgcc tggcctgtgc ataactgtct ggccagcgca cagccgaaga
     3481 gctgcaaaaa gcgcctaccc ttcggtcgct gcgctcccta cgccccgccg cttcgcgtcg
     3541 gcctatcgcg gccgctggcc gctcaaaaat ggctggccta cggccaggca atctaccagg
     3601 gcgcggacaa gccgcgccgt cgccactcga ccgccggcgc ccacatcaag gcaccctgcc
     3661 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     3721 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     3781 ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtatactg
     3841 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat
     3901 accgcacaga tgcgtaagga gaaaataccg cataaggcgc tcttccgctt cctcgctcac
     3961 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     4021 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     4081 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     4141 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     4201 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     4261 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     4321 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     4381 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     4441 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     4501 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     4561 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     4621 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     4681 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     4741 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgcatg atatatctcc
     4801 caatttgtgt agggcttatt atgcacgctt aaaaataata aaagcagact tgacctgata
     4861 gtttggctgt gagcaattat gtgcttagtg catctaacgc ttgagttaag ccgcgccgcg
     4921 aagcggcgtc ggcttgaacg aatttctagc tagacattat ttgccgacta ccttggtgat
     4981 ctcgcctttc acgtagtgga caaattcttc caactgatct gcgcacgagg ccaagcgatc
     5041 ttcttcttgt ccaagataag cctgtctagc ttcaagtatg acgggctgat actgggccgg
     5101 caggcgctcc attgcccagt cggcagcgac atccttcggc gcgattttgc cggttactgc
     5161 gctgtaccaa atgcgggaca acgtaagcac tacatttcgc tcatcgccag cccagtcggg
     5221 cggcgagttc catagcgtta aggtttcatt tagcgcctca aatagatcct gttcaggaac
     5281 cggatcaaag agttcctccg ccgctggacc taccaaggca acgctatgtt ctcttgcttt
     5341 tgtcagcaag atagccagat caatgtcgat cgtggctggc tcgaagatac ctgcaagaat
     5401 gtcattgcgc tgccattctc caaattgcag ttcgcgctta gctggataac gccacggaat
     5461 gatgtcgtcg tgcacaacaa tggtgacttc tacagcgcgg agaatctcgc tctctccagg
     5521 ggaagccgaa gtttccaaaa ggtcgttgat caaagctcgc cgcgttgttt catcaagcct
     5581 tacggtcacc gtaaccagca aatcaatatc actgtgtggc ttcaggccgc catccactgc
     5641 ggagccgtac aaatgtacgg ccagcaacgt cggttcgaga tggcgctcga tgacgccaac
     5701 tacctctgat agttgagtcg atacttcggc gatcaccgct tcccccatga tgtttaactt
     5761 tgttttaggg cgactgccct gctgcgtaac atcgttgctg ctccataaca tcaaacatcg
     5821 acccacggcg taacgcgctt gctgcttgga tgcccgaggc atagactgta ccccaaaaaa
     5881 acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa
     5941 ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagcttac gaaccgaaca
     6001 ggcttatgtc cactgggttc gtgcccgaat tgatcacagg cagcaacgct ctgtcatcgt
     6061 tacaatcagc atgctaccct ccgcgagatc atccgtgttt aaaacccggc agcttagttg
     6121 ccgttcttcc gaatagcatc ggtaacatga gcaaagtctg ccgccttaca acggctctcc
     6181 cgctgacgcc gtcccggact gatgggctgc ctgtatcgag tggtgatttt gtgccgagct
     6241 gccggtcggg gagctgttgg ctggctggtg gcaggatata ttgtggtgta aacaaattga
     6301 cgcttagaca acttaataac acattgcgga tccctcggtc acatgtgcat cctccacaag
     6361 cgtcgatcta gtaacataga tgacaccgcg cgcgataatt tatcctagtt tgcgcgctat
     6421 attttgtttt ctatcgcgta ttaaatgtat aattgcggga ctctaatcat aaaaacccat
     6481 ctcataaata acgtcatgca ttacatgtta attattacat gcttaacgta attcaacaga
     6541 aattatatga taatcatcgc aagaccggca acaggattca atcttaagaa actttattgc
     6601 caaatgtttg aacgatctgc ttgacaagct cagaagaact cgtcaagaag gcgatagaag
     6661 gcgatgcgct gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg gtcagcccat
     6721 tcgccgccaa gctcttcagc aatatcacgg gtagccaacg ctatgtcctg atagcggtcc
     6781 gccacaccca gccggccaca gtcgatgaat ccagaaaagc ggccattttc caccatgata
     6841 ttcggcaagc aggcatcgcc atgggtcacg acgagatcat cgccgtcggg catgcgcgcc
     6901 ttgagcctgg cgaacagttc ggctggcgcg agcccctgat gctcttcgtc cagatcatcc
     6961 tgatcgacaa gaccggcttc catccgagta cgtgctcgct cgatgcgatg tttcgcttgg
     7021 tggtcgaatg ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc atcagccatg
     7081 atggatactt tctcggcagg agcaaggtga gatgacagga gatcctgccc cggcacttcg
     7141 cccaatagca gccagtccct tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga
     7201 acgcccgtcg tggccagcca cgatagccgc gctgcctcgt cctgcagttc attcagggca
     7261 ccggacaggt cggtcttgac aaaaagaacc gggcgcccct gcgctgacag ccggaacacg
     7321 gcggcatcag agcagccgat tgtctgttgt gcccagtcat agccgaatag cctctccacc
     7381 caagcggccg gagaacctgc gtgcaatcca tcttgttcaa tcattgtaat tgtaaatagt
     7441 aattgtaatg ttgtttgttg tttgttgttg ttggtaattg ttgtaaaaat acagtatgag
     7501 agtgaatatg agactctaat tggataccga ggggaattta tggaacgtca gtggagcatt
     7561 tttgacaaga aatatttgct agctgatagt gaccttaggc gacttttgaa cgcgcaataa
     7621 tggtttctga cgtatgtgct tagctcatta aactccagaa acccgcggct gagtggctcc
     7681 ttcaacgttg cggttctgtc agttccaaac gtaaaacggc ttgtcccgcg tcatcggcgg
     7741 gggtcataac gtgactccct taattctccg ctctccgcat gcgaattctc tgagcggtga
     7801 gatttctcag ctggagaagt tcattaggga gcatgtgaaa ccttgattat ggtatttcaa
     7861 gcaaacgggg ggcccctttt tatttccttc tcttttattt ttggtccggg aagctgaaaa
     7921 tactatcaga tagacgaaaa aaacatcaac ttggggaaca ttttccaagt agggatagga
     7981 atttgaagag ttctccttat gttctattgg caatccccac ctaaacatta tgtttgttgt
     8041 gcctagaata tcttgtcatt ggatttgatt acttcccttt atttttgtgc tggttgctct
     8101 tcatggcaaa ggactttcct gtgcataatt tttcttcatt gaattccttt tcctccatgt
     8161 gttgctagcg acattgaaga tgttcttttt tgtgtgcttc tgatacgttt gaactattgg
     8221 agatgtaaaa tagcacttct tttaataatt aagaagtgct ctaacattat ttggcatatg
     8281 gtttgaaact ttggtggata atgagttcca aagagtgcat tttttctgcc aagatttgca
     8341 tccttcatca atatattgat gagcgatgtg tgaagataat atttcttgac aattgaacac
     8401 ttatcttatt gttgactctt tctttgttta cgcctcgatt attctattaa gtacttaata
     8461 tctccattgt gtagaagaat gtttagaatt tttgaatttg tctttcgtcg tttcccatta
     8521 gaagttttta ctgtttgaat tgtgcattgt aatttctgtt aaaagggaga aacaactctc
     8581 cactaaaatc tcatttatgt atccacttat ttaatcttaa gtaagtttaa atgtctattt
     8641 agatgctaat ttagtctaaa tgaaatgata caattatgtt ttaatgcctt tttaaccttt
     8701 tctaaaagac taaaagagga ttcacatatt ttcccgttgg aataaacttc agtagtttgc
     8761 aacacaaact gaaaataata aacaatatga aggtagttac tcctggagat tcaagcaaca
     8821 attacgtacg aggtttggag agggtggtct atatgcaacc ttaaacaggt aattttcgat
     8881 atcctcgact caagcagaac ctatcaatcc tcgactcaag cagaacctat caaatatgaa
     8941 aaggatatac aaaggagatc caagaatgtg gaagtaaaat catttatctg agcaacatta
     9001 agaatttaaa aggaaaaaaa aagacattat ggtttaagaa tcactagcgt ataataatgt
     9061 ttttttgaat tgtttaatgg tatttgaact cattgtttgt ctccaaatag ctttctaatc
     9121 atccatttga acaatcaata cttcaatgct atcaccaagt catatatgcc atagcaaggg
     9181 aaccatcaag tacccacgca attacacatt tggcaaagag caccaagcat aacgataagc
     9241 acttaacgaa ggttttaatc ctgttaagtt tttgtgcttt taggtctgag cattttgatc
     9301 aagttacttg aagaaataga atatgaagat cggaatattt cttcaaatat tggttcgtag
     9361 taacatattg tatttaaaaa atagtagtat atataaaaat gatttatgat atgaaaaatt
     9421 agttaataca ttaatggacc taatttatat tgtgaatgtt agttgattaa aagagtgcat
     9481 ttcataactc caaaattcca ccatgttagt cattaaatcg acattcaacg aaaagaagat
     9541 accaaaaaca aaaaggctca cgcattttat tagagtacta gaaaatcaag aggctgcaac
     9601 attaagcact taaaagataa atacatacac atatattaaa ttatgattaa caaaacacag
     9661 aactttaaaa agagtactcc agattttaac aaatcaagct catttgtaca gctcgtccat
     9721 gccattaagt ttgtgcccca gtttgctagg gaggtcgcag tatctggcca cagccacctc
     9781 gtgctgctcg acgtaggttt ctttgtcggc ctccttgatt ctttccagtc tgtggtccac
     9841 atagtagacg ccgggcatct tgaggttctt agcgggtttc ttggatctgt atgtggtctt
     9901 gaagttgcag atcaggtggc ccccgcccac gagcttcagg gccatgtcgg ttctgccttc
     9961 caggccgccg tcagcggggt acagcatctc ggtgttggcc tcccagccga gtgttttctt
    10021 ctgcatcaca gggccgttgg atgggaagtt cacccctctg atcttgacgt tgtagatgag
    10081 gcagccgtcc tggaggctgg tgtcctgggt agcggtcagc acgcccccgt cctcgtatgt
    10141 ggtgactctc tcccatgtga agccctcagg gaaggactgc ttaaagaagt cggggatgcc
    10201 ctgggtgtgg ttgatgaagg ttctgctgcc gtacatgaag ctggtagcca ggatgtcgaa
    10261 ggcgaagggg agagggccgc cctcgaccac cttgattctc atggtctggg tgccctcgta
    10321 gggcttgcct tcgccctcgg atgtgcactt gaagtggtgg ttgttcacgg tgccctccat
    10381 gtacagcttc atgtgcatgt tctccttaat cagctcttcg cccttagaca cggcagccga
    10441 agctttgtac agctcgtcca tgccattaag tttgtgcccc agtttgctag ggaggtcgca
    10501 gtatctggcc acagccacct cgtgctgctc gacgtaggtt tctttgtcgg cctccttgat
    10561 tctttccagt ctgtggtcca catagtagac gccgggcatc ttgaggttct tagcgggttt
    10621 cttggatctg tatgtggtct tgaagttgca gatcaggtgg cccccgccca cgagcttcag
    10681 ggccatgtcg gttctgcctt ccaggccgcc gtcagcgggg tacagcatct cggtgttggc
    10741 ctcccagccg agtgttttct tctgcatcac agggccgttg gatgggaagt tcacccctct
    10801 gatcttgacg ttgtagatga ggcagccgtc ctggaggctg gtgtcctggg tagcggtcag
    10861 cacgcccccg tcctcgtatg tggtgactct ctcccatgtg aagccctcag ggaaggactg
    10921 cttaaagaag tcggggatgc cctgggtgtg gttgatgaag gttctgctgc cgtacatgaa
    10981 gctggtagcc aggatgtcga aggcgaaggg gagagggccg ccctcgacca ccttgattct
    11041 catggtctgg gtgccctcgt agggcttgcc ttcgccctcg gatgtgcact tgaagtggtg
    11101 gttgttcacg gtgccctcca tgtacagctt catgtgcatg ttctccttaa tcagctcttc
    11161 gcccttagac accattggac ggtggtggag aaggtcgaat ttgaatttat ttaataaaaa
    11221 taaaatgaat tgaggatgag aaaaaagggg aaggaggtgg gagtgaatat aaaggaagag
    11281 gagaatggac gcgtggagaa gagtgacaga tgttgatgag aaagtttgca tgaaaaaaat
    11341 ggatttagca gaaagagaga tgagtttgaa agtaaagagt ctgtttttac gattttggct
    11401 ttttctctgc ctctctgttt ctttgttatt ttttgaggga ttttcgacac ttcaaaggga
    11461 aaataataat taacactctt agtcccttga gtataaataa atttaaactt tggtcatcgt
    11521 gaattaatat gtgtgcattt ggatctctct gactttgaat cttcacaatt ttaatgaact
    11581 gttgagaatt aaactattga attattcatg tacttcgtta aaattttaag tataatttta
    11641 ataaatttca tattgtttct cttttttgaa ttataatttt ttttaaaaaa tttatgaatt
    11701 tcgaatacat tcaaatacag aagtgaaggt gtatcgagag gggagggatg gattagaaaa
    11761 agaataggac attcaaatac atcattggac tttcagatac attagcagac attcggatac
    11821 ataaagagaa gatataccag agatcgagaa ggaataaaaa tatattaaca agagtagagc
    11881 aatgaatttg agagaatatt tttgattttt taaaaatgat tgaaaattta agagattatg
    11941 taaaataaaa tgtgtgtgta tataattttt ccaaaatttt gttgttatta tatgagaaaa
    12001 atttaataca ttacttatat gtttcctact aaaaatatgg gctagtaaat gggcttagtc
    12061 aggaattgga gggtttttct gttgtgggcc taatgggcta catttgggag ctattttggg
    12121 cctttctata atatgatgct ggattgggct attttaacaa tgttatgtat tggattctaa
    12181 ttggacttta gaggatccat gcaaggtgca tccttgtaga ctgtactccc catgggaatt
    12241 cgtaaagcgg tcctgctgag cctcgacatg ttgtcgcaaa attcgccctg gacccgccca
    12301 acgatttgtc gtcactgtca aggtttgacc tgcacttcat ttggggccca catacaccaa
    12361 aaaaatgctg cataattctc ggggcagcaa gtcggttacc cggccgccgt gctggaccgg
    12421 gttgaatggt gcccgtaact ttcggtagag cggacggcca atactcaact tcaaggaatc
    12481 tcacccatgc gcgccggcgg ggaaccggag ttcccttcag tgagcgttat tagttcgccg
    12541 ctcggtgtgt cgtagatact agcccctggg gcacttttga aatttgaata agatttatgt
    12601 aatcagtctt ttaggtttga ccggttctgc cgcttttttt aaaattggat ttgtaataat
    12661 aaaacgcaat tgtttgttat tgtggcgctc tatcatagat gtcgctataa acctattcag
    12721 cacaatatat tgttttcatt ttaatattgt acatataagt agtagggtac aatcagtaaa
    12781 ttgaacggag aatattattc ataaaaatac gatagtaacg ggtgatatat tcattagaat
    12841 gaaccgaaac cggcggtaag gatctgagct acacatgctc aggtttttta caacgtgcac
    12901 aacagaattg aaagcaaata tcatgcgatc ataggcttct cgcatatctc attaaagcag
    12961 gacaagccta catttgttct ttccaaattt tggcaatatc ttgtgcaaca ttagtggcat
    13021 ctatagaagc accaaacagt cctattcctg taaatccaac tgcatacaga ccatcctccc
    13081 ccttccaacc atttgggaat gggctttttg gacatccctc ccttgaaaac aattcactct
    13141 cctgtatcag taaaattctc attagaccaa atttcgaagg gggctattat agaacgagag
    13201 aaagttgctc agatggtaaa gagcccctta ttcccaatct taagattgtg agttcgagtt
    13261 atcaaaagaa caaaaaggta tgaggaaagg gtaaaagaaa aaagacggtt atagtgaatt
    13321 ctaactaatt aataactcaa aggaaagaat ccacaggata atgagatacc aacttacatg
    13381 aatgtaacaa gaatccataa ataattacta cattcattaa gagttcattt gactaaatca
    13441 tcctttatag ttgaaggagc ttttatcata cttagtacta tatataattt atgattaaac
    13501 accaatttaa ttaggcatag aaataaaact tgtgtatttc cttaccatta accaagaagt
    13561 tacattgctg gtataaccag ttgccaagat aacagagtcg atctctagaa tttgtccatt
    13621 aacaaattct acttttcctt gagtaaattt cttgattgct ggaactatgt ttatttcctc
    13681 agggaaatta cgaccctgta cctgaaagat taacaaaact agatgatatc attcatgtgt
    13741 gaatataata tattcatgcg agcaaatgaa agatacaaga tcactttcta gtgttacttg
    13801 aaaaataata cttattgaat ataaaactta ccgagcttcg aactaccatg aatggatttg
    13861 cattatgttg ggaaagatca agtgagatat cgatcccgga attgccacag ccaaccgcca
    13921 gcacattttc tccagtatag tattccccag tcttgtactc acaagcatga ataacctggc
    13981 caccaaaatc ttgcaatcct tcgaattcgg gcactatcat ctcagcattc tctcctgtgg
    14041 ccacaataag ccacttacac atgtattcag aggttgaacc attgatttca gaaactgttt
    14101 tcaccttcca taaaccacat gtctcatcat atccagctaa gtttactgac tcgttgagtt
    14161 gtggtttgat ctcgaaatgc tttgcataag atacgaggta gctgatgaat tggtttttgg
    14221 ttggatactc tggaaagtct ggtggaaatg gcaagccagg caattcgcag tattgtcgtg
    14281 gcacgttaag cctaagccga tcgtaggtct tgtgttgcca cagagaagca atgcagtccg
    14341 cgcgttcaat gattacatac ggaacactgt attgcttaag gacagcagca gtagccaggc
    14401 ctgaagggcc agctccaaca ataagaggac catttacttg tatgcattta gcagcacaag
    14461 aggatttttt tggttcaagt gcatcaacag ttagaggtga acaagaatta aagcaattct
    14521 gattcatcat taaatatatg tgcaactagg actacttgtg ttttgataga tttagcgggt
    14581 gacaagaaca agaggagaaa agagaaaggg gatgataact tgaagttgtg aggatggtta
    14641 ggtttatata ataaaattgg tcaggcaaac gtgttcattg tttaaccagg gtgtgcaaat
    14701 tgtggtttaa cccataaact gaaccagaaa tggttaatct atcggttaaa cggttttatt
    14761 gttttttttt ttttttngat atattgatct ttcagttcaa tgattagttc tttgagttta
    14821 cttgcatact aatttcatat ttcccttgca taagtttcgt tagcttgact atgatatatt
    14881 agagtttgct ataagggaaa agatatacaa atgtatcgtt gtatctgtgg taaaaagcaa
    14941 gtacaatcta atgtgtcatg tatcattcgc atttcaaagt aaaactaagg agtttagtct
    15001 aggttgatat taatagactg ttgttttgca ttaattcttt gttgcggttg gcagtaccaa
    15061 gcgttggtaa gggaaatgat tgaattctat tttacttgat atttttttct agttggaaac
    15121 tcaggaacaa atgttttctt actagaaaaa ataattagaa acaaaagcag catatgagga
    15181 atcttcaaat acatgtgaat tagaactctt gacaccaaaa gtttcaacgc aacaaggaga
    15241 tgagcaaatg tgatctccac atttgcttcc tatatcactc tctcattaca agcagcaaac
    15301 cacaacaaaa aatttcgcct atgataaagt atgcttgatt tcactccaca aggcatccca
    15361 aagatgttga tctagccgtc actctaagcc accaaacaca gacaccatta atttatcatg
    15421 atacttttct ggataaattc tgaaaaccag agccaagaca catgagattg acagttcctg
    15481 tggaggcatc aggtttcatg acaaacttaa cacccaaaaa atctctgatg tgccttaatg
    15541 ctgctccgag ctcgaattct agtagcgttg tcaatcaatt ggcaagtcat aaaatgcatt
    15601 aaaaaatatt ttcatactca actacaaatc catgagtata actataatta taaagcaatg
    15661 attagaatct gacaaggatt ctggaaaatt acataaagga aagttcataa atgtctaaaa
    15721 cacaagagga catacttgta ttcagtaaca tttgcagctt ttctaggtct gaaaatatat
    15781 ttgttgccta gtgaataagc ataatggtac aactacaagt gttttactcc tcatattaac
    15841 ttcggtcatt agaggccacg atttgacaca tttttactca aaacaaaatg tttgcatatc
    15901 tcttataatt tcaaattcaa cacacaacaa ataagagaaa aaacaaataa tattaatttg
    15961 agaatgaaca aaaggaccat atcattcatt aactcttctc catccatttc catttcacag
    16021 ttcgatagcg aaaaccgaat aaaaaacaca gtaaattaca agcacaacaa atggtacaag
    16081 aaaaacagtt ttcccaatgc cataatactc aaactcagta ggattctggt gtgtgcgcaa
    16141 tgaaactgat gcattgaact tgacgaacgt tgtcgaaacc gatgatacga acgaaagctc
    16201 taagctcaga ctttacgctt cttctttggg tcagccctag agtcaccacc caactgcgac
    16261 agatctatcc ttgtctcata gagccctgta atagattggt gaataagggt agcgtccaac
    16321 acttccttgg ttgatgtgta gcgctttctg tcgatagtgg tatcgaagta cttaaaggct
    16381 gctggggctc ccagattggt cagtgtgaac aggtggatga tgttttccgc ttgttccctt
    16441 atcggcttat ctctgtgctt gttgtacgcg gaaagcacct ttaattgtag aaaaccagta
    16501 aaacatggac aattaggagg catcgattat ctaaccaaag actttaacaa cactaatctg
    16561 ccactaatat tcatgagaag tccttacctt atcgaggtta gcgtctgcaa gaatgacccg
    16621 cttggaaaat tcgctgatct gctctatgat ctcatcaaga tagtgcttgt gctgctcgac
    16681 aaacaactgc ttctgctcgt tgtcctctgg tgaccccttc agcttctcat agtgtgaggc
    16741 caagtacagg aagttgacgt acttactggg caatgccagc tcgttgccct tctgcagttc
    16801 tccggcagat gccagcatgc gttttctgcc attttccaat tcgaaaagcg aatacttggg
    16861 cagtttgata atgaggtctt tcttcacctc tttgtaacct ggtttgtata attttggttc
    16921 agttaattag gaaaattaca taatggctcc aaaaaaaaat tatcgacctt ttaccaaaaa
    16981 aaataaaatt tatcgacctt gacaggaaaa agttacaaat caaaagaaag aatatcgtct
    17041 ggcatccaat attttacctt tagcctcgag gaagtcgatc ggattcttct cgaacgagga
    17101 cctttccatt atggtaattc caagcagttc cttgacgctt ttcagtttct tgctcttgcc
    17161 cttctccacc ttggccacta ccagtacgct gtatgccacg gtagggctat caaagcctcc
    17221 gtatttcttc ggatcccaat ccttcttcct cgcaatcagt ttgtcggagt tgcgcttagg
    17281 gagaatggac tccttggaga aaccaccggt ttgaacctcc gttttcttga ctatgttgac
    17341 ttggggcatg ctgagaacct tacgcacagt cgcaaaatcc ctgcccttat cccagacaat
    17401 ctcacctgca agaaacagca aagagagagt taagatataa gtgaacaaga aagtcttggg
    17461 atcgacatac atacaatcaa ttgatcaaat tcctaggatt aaacatatga aataggaaag
    17521 acagacctgt ttcgccattt gtctcgatca gaggcctctt ccggatttca ccattcgcca
    17581 gagtgatctc tgtcttgaag aagttcatga tattcgagta aaagaagtat ttcgcggtag
    17641 ccttgccgat ctcttgttct gacttcgcga tcatcttcct aacgtcgtac accttgtaat
    17701 ccccgtacac aaactcagac tccagtttcg gatacttctt gatgagagca gtgccaacaa
    17761 cggcgttcag gtaggcgtca tgggcatgat ggtagttgtt gatctcgcga accttgtaaa
    17821 actggaagtc cttccggaag tcactaacca gtttgctctt gagggtgatc acctgcgtaa
    17881 ccatcaaaag tcaatatgga ttcatcacaa attaatcagg aatagaaaga tcgggtggcc
    17941 ttgaagttga tcaaataaaa agtttcgaac tttagtttga tatggtaaag gaaagttgga
    18001 aactttacct ttacctccct gataagtttg tcattctcat catacttcgt attcatacgc
    18061 gaatcgagta tctgggccac atgtttcgta atctgacgag tttcaaccaa ctggcgcttg
    18121 atgaagccgg ctttgtcgag ctcagacagt ccgccccttt ctgccttcgt gagattgtcg
    18181 aatttccgtt gggttatgag cttagcattc aaaagctgcc tccaatagtt cttcatcttc
    18241 ttcacaacct cctcgctggg tacattgtcg gattttccgc ggttcttgtc tgagcgcgta
    18301 aggacctata acacgggaga aaccaaaaag caaactcatt tcacgccaaa tactacagat
    18361 ttctcaaaaa ttaagttata atccatatat cctgacaaga aattgattaa aacacagttg
    18421 ctttaccttg ttgtcaatac tatcgtcctt caggaaagat tgagggacaa tgtgatccac
    18481 atcatagtcg gagagtctgt taatgtcgag ctcttggtcg acgtacatat ctctcccatt
    18541 ctggaggtaa tagaggtaca gcttctcgtt ctggagttga gtattttcga ctgggtgctc
    18601 tttcagaatc tgactcccaa gctccttgat gccttcctct atccgcttca tgcgctcccg
    18661 tgagttcttc tgccctttct gagtcgtctg gttttccctc gccatctcga tgacgatgtt
    18721 ctccggctta tggcggccca tgacctgtag caataattaa aacaaaaata ccacaaacag
    18781 ttagaactga ggcaagaaga atgaatgcag agaaaaaaaa tcttagctat tcatggaaat
    18841 ggaagtgcga cgaacaccta aaatgcaagg agcataacca aatgcagaac ttaccttcac
    18901 cagctcatcc acaaccttca ctgtctgcag tatgcccttc tttatagctg gcgatccagc
    18961 caggttggct atgtgttcgt gaaggctatc gccctgacct gacacttggg ccttctggat
    19021 atcttctttg aaggtcagtg agtcatcgtg aataagctgc atgaagttcc gattcgcaaa
    19081 accgtcggat ttgagaaagt ccagtatagt cttgccggat tgcttgtcgc ggatgccgtt
    19141 gatgagcttc ctggagagcc ttccccaacc taaaacatag aacaaacatt tgtctcatca
    19201 caaatatgca tcaaggaaca ctcagaagtt agtcatatac ccaaattctt gaaactagag
    19261 aaacaaaaaa agagggatca ttgcggaaac cttttgacct cttacctgta tatctcctcc
    19321 tcttcaactg cttcatgacc ttgtcatcga acagatgcgc ataagtctta aggcgctcct
    19381 cgatcatttc tctgtcctca aacagcgtga gagtcagcac gatgtcctcg aggatgtcct
    19441 cgttctcctc gttgtcaagg aagtccttgt ccttgatgat tttcaacagg tcatggtatg
    19501 tgccaagaga ggcattgaac ctatcctcaa cgccgctgat ttccacagaa tcaaagcatt
    19561 cgatcttctt aaagtagtcc tctttaagct gtttcacagt gaccttccga ttggttttga
    19621 acaagagatc gacaatggcc ttcttctgct cacctgttat aacagtaaaa attactttag
    19681 aaacaaaaca aaagtgtggt cttttaggaa tgattattga tcagttcata gtttaaaatc
    19741 ttgtgttttt atcgtaaaag tgtggaccag gatttacctg acaggaaggc cggtttgcgc
    19801 atcccctccg tcacatattt caccttcgtc agctcgttgt agaccgtgaa gtattcgtac
    19861 aggagactat gcttgggcag gactttctca tttgggagat tcttatcgaa gttggtcatc
    19921 ctttcgatga aggactgtgc actcgcaccc ttgtccacga cctcttcgaa gttccaaggc
    19981 gtgatagtct cctcggattt gcgtgtcatc cacgcaaacc gcgagttccc gcgtgcgagt
    20041 ggacctaatg aattaataga aaaatcaggc aaattagaaa agaaataatt ctcaaaagag
    20101 aacaactgag gtcagaaaat agcattcatc aactgaaact aggtatttct tcatatgata
    20161 ctaacctaca tagtagggaa tgcggaacgt gaggatcttt tcaatcttct ctctgttgtc
    20221 tttcaggaac ggatagaagt cctcttgcct cctcaagata gcatgcagct caccgaggtg
    20281 gatctggtgc ggtatgctgc cattgtcgaa ggtacgctgc ttccgaagca aatcttcgcg
    20341 gttcaattta acgagcagct cctctgtacc gtccatcttt tccagaatag gcttgatgaa
    20401 tttgtagaat tcctcctgac ttgcacctaa taatatcatc agaggaaaac tcaattctta
    20461 tatcttacag aaatgaacaa caaaatacat gtagcagaga ggaaaacaat cataagtaac
    20521 aacttacctc catcaatgta ccctgcgtag ccattcttcg attggtcaaa gaaaatctcc
    20581 ttgtacttct cggggagttg ctggcgaacc aaagccttca gaagggtcag atcctggtga
    20641 tgctcgtcgt acctcttgat catggaggcg ctaagtggcg ctttggttat ctccgtgttg
    20701 acccgcagaa tgtcactgag caagatagcg tctgacagat tcttggcagc caagaacagg
    20761 tcggcatact gatcacctac aaaagtttaa aagactcaat tttaactcta cagcttcaaa
    20821 taaatttccg caaagaaatt gataggttta gacaacaaaa gaagagttcc aatttcaaga
    20881 gcacctattt gggcgagcag attgtccaga tcatcatcgt atgtatcctt tgacagctgc
    20941 agcttcgcgt cctcggcgag gtcaaagttg cttttgaagt taggggtgag gcccagagac
    21001 agtgcaatca gattcccgaa caagccgttc ttcttctcgc ctggcaattg agcgatcaga
    21061 ttctcaagcc gtctggactt cgaaagtctg gcagacagta ttgccttggc gtcgacacct
    21121 gagattgttt gcaaagaaaa atctcaaagc atgtacaatc tataaactta tgatggaaaa
    21181 agaaaagaaa ggtaactaag gaatgttacc tgatgcattg atggggttct cctcgaacag
    21241 ctgattgtag gtttggacga gctgaatgaa cagtttatcc acatcggagt tatccgggtt
    21301 gaggtcccct tcgatcaaga aatggccacg aaactttatc atgtgcgcga gagccaggta
    21361 tatgagccga aggtctgcct tatcggttga gtccaccagc ttcttgcgaa ggtggtatat
    21421 agtcggatac ttttcatgat aggctacttc gtcaacaata ttcccaaaga tggggtgacg
    21481 ctcgtgtttc ttatcttcct ctaccaggaa ggattcctcg aggcgatgga agaacgagtc
    21541 gtcaacctgc aaaatcaagt ttattgatca tcaagtgagt ttacatgtag ttaaatgctt
    21601 tcagtaatat cccaatgaag caggatatgc tttcagtaca aaatacataa gcatactata
    21661 tcataaaaat ccttaccttc gccatctcgt tgctaaagat ctcttggaga taacagattc
    21721 tgttcttcct gcgagtgtac cttctcctcg cagtacgttt aagccttgtc gcctccgcgg
    21781 tttctccaga gtcaaagagc agagctccga tcaagttctt cttaatgctg tgcctatctg
    21841 tgttgcccag cactttaaat ttcttcgaag gtaccttgta ttcatcggtg ataacggccc
    21901 acccaactga gttcgtacca atatcaagtc caatgctgta cttcttgtcc attgcagatt
    21961 tgtacagctc gtccatgccg agagtgatcc cggcggcggt cacgaactcc agcaggacca
    22021 tgtgatcgcg cttctcgttg gggtctttgc tcagcttgga ctgagtactc aggtagtggt
    22081 tgtcgggcag cagcacgggg ccgtcgccga tgggggtgtt ctgctggtag tggtcggcga
    22141 gctgcacgct gccgtcctcg atgttgtggc ggatcttgaa gttcaccttg atgccgttct
    22201 tctgcttgtc ggccatgata tagacgttgt ggctgttgta gttgtactcc agcttgtgcc
    22261 ccaggatgtt gccgtcctcc ttgaagtcga tgcccttcag ctcgatgcgg ttcaccaggg
    22321 tgtcgccctc gaacttcacc tcggcgcggg tcttgtagtt gccgtcgtcc ttgaagaaga
    22381 tggtgcgctc ctggacgtag ccttcgggca tggcggactt gaagaagtcg tgctgcttca
    22441 tgtggtcggg gtagcggctg aagcactgca cgccgtaggt cagggtggtc acgagggtgg
    22501 gccagggcac gggcagcttg ccggtggtgc agatgaactt cagggtcagc ttgccgtagg
    22561 tggcatcgcc ctcgccctcg ccggacacgc tgaacttgtg gccgtttacg tcgccgtcca
    22621 gctcgaccag gatgggcacc accccggtga acagctcctc gcccttgctc actgcggcag
    22681 ctgagtcgag tttaactctt ttggcagcag gagccatggt atcgataatt gtaaatgtaa
    22741 ttgtaatgtt gtttgttgtt tgttgttgtt ggtaattgtt gtaaaaatga gctcttatac
    22801 tcgagcgtgt cctctccaaa tgaaatgaac ttccttatat agaggaaggg tcttgcgaag
    22861 gatagtggga ttgtgcgtca tcccttacgt cagtggagat gtcacatcaa tccacttgct
    22921 ttgtagacgt ggttggaacc tcttcttttt ccacgatgct cctcgtgggt gggggtccat
    22981 ctttgggacc actgtcggca gagagatctt gaatgatagc ctttccttta tcgcaatgat
    23041 ggcatttgta ggagccacct tccttttcta ctgtcctttc gatgaagtga cagatagctg
    23101 ggcaatggaa tccgaggagg tttcccgaaa ttatcctttg ttgaaaagtc tcaatagccc
    23161 tttgatcttc tgagactgta tctttgacat ttttggagta gaccagagtg tcgtgctcca
    23221 ccatgttgac gaagattttc ttcttgtcat tgagtcgtaa aagactctgt atgaactgtt
    23281 cgccagtctt gacggcgagt tctgttagat cctcgatttg aatcttagac tccatgcatg
    23341 gccttagatt cagtaggaac taccttttta gagactccaa tctctattac ttgccttggt
    23401 ttatgaagca agccttgaat cgtccatact ggaatagtac ttctgatctt gagaaatatg
    23461 tctttctctg tgttcttgat gcaattagtc ctgaatcttt tgactgcatc tttaaccttc
    23521 ttgggaaggt atttgatctc ctggagattg ttactcgggt agatcgtctt gatgagactt
    23581 gctgcgtagg cctctctaac catctgtggg tcagcattct ttctgaaatt gaagaggcta
    23641 accttctcat tatcagtggt gaacatagtg tcgtcacctt caccttcgaa cttccttcct
    23701 agatcgtaaa gatagaggaa atcgtccatt gtaatctccg gggcaaagga gatctctttt
    23761 ggggctggat cactgctggg ccttttggtt cctagcgtga gccagtgggc tttttgcttt
    23821 ggtgggcttg ttagggcctt agcaaagctc ttgggcttga gttgagcttc tcctttgggg
    23881 atgaagttca acctgtctgt ttgctgactt gttgtgtacg cgtcagctgc tgctcttgcc
    23941 tctgtaatag tggcaaattt cttgtgtgca actccgggaa cgccgtttgt tgccgccttt
    24001 gtacaacccc agtcatcgta tataccggca tgtggaccgt tatacacaac gtagtagttg
    24061 atatgagggt gttgaatacc cgattctgct ctgagaggag caactgtgct gttaagctca
    24121 gatttttgtg ggattggaat tcctccaagc ttgaattctt gcagagaccg agacgttgat
    24181 cggcacgtaa gaggttccaa ctttcaccat aatgaaataa gatcactacc gggcgtattt
    24241 tttgagttat cgagattttc aggagctaag gaagctaaaa tggagaaaaa aatcactgga
    24301 tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc atttcagtca
    24361 gttgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt tttaaagacc
    24421 gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc ccgcctgatg
    24481 aatgctcatc cggaattccg tatggcaatg aaagacggtg agctggtgat atgggatagt
    24541 gttcaccctt gttacaccgt tttccatgag caaactgaaa cgttttcatc gctctggagt
    24601 gaataccacg acgatttccg gcagtttcta cacatatatt cgcaagatgt ggcgtgttac
    24661 ggtgaaaacc tggcctattt ccctaaaggg tttattgaga atatgttttt cgtctcagcc
    24721 aatccctggg tgagtttcac cagttttgat ttaaacgtgg ccaatatgga caacttcttc
    24781 gcccccgttt tcaccatggg caaatattat acgcaaggcg acaaggtgct gatgccgctg
    24841 gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg tcggcagaat gcttaatgaa
    24901 ttacaacagt actgcgatga gtggcagggg gggcgtaaac gccgcgtgga tccggcttac
    24961 taaaagccag ataacagtat gcgtatttgc gcgctgattt ttgcggtata agaatatata
    25021 ctgatatgta tacccgaagt atgtcaaaaa gaggtatgct atgaagcagc gtattacagt
    25081 gacagttgac agcgacagct atcagttgct caaggcatat atgatgtcaa tatctccggt
    25141 ctggtaagca caaccatgca gaatgaagcc cgtcgtctgc gtgccgaacg ctggaaagcg
    25201 gaaaatcagg aagggatggc tgaggtcgcc cggtttattg aaatgaacgg ctcttttgct
    25261 gacgagaaca ggggctggtg aaatgcagtt taaggtttac acctataaaa gagagagccg
    25321 ttatcgtctg tttgtggatg tacagagtga tattattgac acgcccgggc gacggatggt
    25381 gatccccctg gccagtgcac gtctgctgtc agataaagtc tcccgtgaac tttacccagt
    25441 ggtgcatatc ggggatgaaa gctggcgcat gatgaccacc gatatggcca gtgtgccggt
    25501 ttccgttatc ggggaagaag tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc
    25561 cattaacctg atgttctggg gaatataaat gtcaggctcc cttatacaca gccagtcggt
    25621 ctcagcttct gcaggcatgc gacgtcgagc ttagcttgag cttggatcag attgtcgttt
    25681 cccgccttca gtttaaacta tcagtgtttg acaggatata ttggcgggta aacctaagag
    25741 aaaagagcgt ttattagaat aatcggatat ttaaaagggc gtgaaaaggt ttatccgttc
    25801 gtccatttgt atgtgcatgc caaccacagg gttcccctcg ggatcaaagt actttaaagt
    25861 actttaaagt actttaaagt actttgatcc aacccctccg ctgctatagt gcagtcggct
    25921 tctgacgttc agtgcagccg tgttctgaaa acgacatgtc gcacaagtcc taagttacgc
    25981 gacaggctgc cgccctgccc tt
//
