LOCUS       sequence-333328-        3356 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 module (CDS1) for further assemblies based on the Modular 
            Cloning system. FCY-UPP gene conferring sensitivity to 
            5-Fluorocytosine.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3356)
  AUTHORS   Stuttmann J, Barthel K, Martin P, Ordon J, Erickson JL, Herr R, 
            Ferik F, Kretschmer C, Berner T, Keilwagen J, Marillonnet S, Bonas U
  TITLE     Highly efficient multiplex editing: One-shot generation of 8x 
            Nicotiana benthamiana and 12x Arabidopsis mutants.
  JOURNAL   Plant J. 2021 Feb 12. doi: 10.1111/tpj.15197.
  PUBMED    33577114
REFERENCE   2  (bases 1 to 3356)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2021 Feb 12. doi: 10.1111/tpj.15197."
COMMENT     Sequence Label: pJOG1368
FEATURES             Location/Qualifiers
     source          1..3356
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     230..247
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1470..1488)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1556..1660
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1882..2673
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(2844..3356,1..76)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3333..3352
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
       61 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
      121 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
      181 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
      241 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
      301 tcattaatca ctctgtggtc tcaaatggtg acagggggaa tggcaagcaa gtgggatcag
      361 aagggtatgg acattgccta tgaggaggcg gccttaggtt acaaagaggg tggtgttcct
      421 attggcggat gtcttatcaa taacaaagac ggaagtgttc tcggtcgtgg tcacaacatg
      481 agatttcaaa agggatccgc cacactacat ggtgagatct ccactttgga aaactgtggg
      541 agattagagg gcaaagtgta caaagatacc actttgtata cgacgctgtc tccatgcgac
      601 atgtgtacag gtgccatcat catgtatggt attccacgct gtgttgtcgg tgagaacgtt
      661 aatttcaaaa gtaagggcga gaaatattta caaactagag gtcacgaggt tgttgttgtt
      721 gacgatgaga ggtgtaaaaa gatcatgaaa caatttatcg atgaaagacc tcaggattgg
      781 tttgaagata ttggtgaggc ttccgccaag atcgtggaag tcaaacaccc actcgtcaaa
      841 cacaagctgg gactgatgcg tgagcaagat atcagcacca agcgctttcg cgaactcgct
      901 tccgaagtgg gtagcctgct gacttacgaa gcgaccgccg acctcgaaac ggaaaaagta
      961 actatcgaag gctggaacgg cccggtagaa atcgaccaga tcaaaggtaa gaaaattacc
     1021 gttgtgccaa ttctgcgtgc gggtcttggt atgatggacg gtgtgctgga aaacgttccg
     1081 agcgcgcgca tcagcgttgt cggtatgtac cgtaatgaag aaacgctgga gccggtaccg
     1141 tacttccaga aactggtttc taacatcgat gagcgtatgg cgctgatcgt tgacccaatg
     1201 ctggcaaccg gtggttccgt tatcgcgacc atcgacctgc tgaaaaaagc gggctgcagc
     1261 agcatcaaag ttctggtgct ggtagctgcg ccagaaggta tcgctgcgct ggaaaaagcg
     1321 cacccggacg tcgaactgta taccgcatcg attgatcagg gactgaacga gcacggatac
     1381 attattccgg gcctcggcga tgccggtgac aaaatctttg gtacgaaata gctttgagac
     1441 cacgaagtgg ctcttcagtg gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     1501 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     1561 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     1621 accctgataa atgcttcaat aatattgaaa aaggaagagt atgcgctcac gcaactggtc
     1681 cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta
     1741 tgactgtttt tttttggggt acagtctatg cctcgggcat ccaagcagca agcgcgttac
     1801 gccgtgggtc gatgtttgat gttatggagc agcaacgatg ttacgcagca gggcagtcgc
     1861 cctaaaacaa agttaaacat catgagggaa gcggtgatcg ccgaagtatc gactcaacta
     1921 tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg
     1981 tacggctccg cagtggatgg cggcctgaag ccacacagcg atattgattt gctggttacg
     2041 gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact
     2101 tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac
     2161 gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag
     2221 cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc
     2281 ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc
     2341 tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg
     2401 aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt
     2461 tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg
     2521 gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga
     2581 caagaagaag atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt ccattacgta
     2641 aaaggcgaga tcaccaaggt agtcggcaaa taactgtcag accaagttta ctcatatata
     2701 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     2761 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     2821 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     2881 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     2941 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     3001 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     3061 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     3121 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     3181 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     3241 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     3301 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatag
//
