LOCUS       sequence-33333-e         758 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 758)
  AUTHORS   Kim JH, Wei JR, Wallach JB, Robbins RS, Rubin EJ, Schnappinger D
  TITLE     Protein inactivation in mycobacteria by controlled proteolysis and 
            its application to deplete the beta subunit of RNA polymerase.
  JOURNAL   Nucleic Acids Res. 2011 Mar;39(6):2210-20. doi: 10.1093/nar/gkq1149
  PUBMED    21075796
REFERENCE   2  (bases 1 to 758)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2011-03"; volume: "39"; issue: "6"; 
            pages: "2210-20"
COMMENT     Sequence Label: pGMCZ-PrpoB
FEATURES             Location/Qualifiers
     source          1..758
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(101..119)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(102..119)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    182..202
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    208..232
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cttatctagc cgaaagcttg aacaccagat ggacacgctc caggactact acatggttgc
       61 gcggcacgta ccgatgccag ctaggccatc gaattccact tctatagtgt cacctaaatc
      121 accggttata aaaaattttt aaaataaaaa aggggacctc tagggtcccc tgcagcttaa
      181 gcaactatgt ataataaagt tgacgcgtcc actttgtaca agaaagctgg gtgcgggtgt
      241 tgaaacttga ctactgaggc ggtcttcgga cgaggctcta cgcgagatcc tcgacggacg
      301 cggattcgtt gcgcgacagg ttgattccca ggttcgcggc agcgcgctcc aggtcctcgt
      361 cgtcaccgtc acgcatctcg atcgccgcgc cgtcgctgga gagcacctcg acgttgaggc
      421 acagcgactg cagctccttg agcaacacct tgaacgactc ggggataccg ggttcaggga
      481 tgttctcgcc cttgacgatc gcctcgtaca ccttgacgcg gcccaccgtg tcgtcggact
      541 tgatggtcag cagttcctgc agcgtgtagg cagcgccgta cgcctgcatg gcccaacatt
      601 ccatctcgcc gaagcgctga ccaccgaact gcgccttacc accgagcggc tgctgggtga
      661 tcatcgagta cggaccggtc gaacgcgcgt ggatcttgtc gtcgaccagg tggtgcagct
      721 tcaggatgta catgtagcca accgtcaccg ggtacggg
//
