LOCUS       sequence-333330-        3302 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 module (Pro+5U) for further assemblies based on the Modular 
            Cloning system. Promoter and 5'UTR of a Solanum lycopersicum Oleosin
            gene, arbitrarily called OLE2 (Sollyc03g112440).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3302)
  AUTHORS   Stuttmann J, Barthel K, Martin P, Ordon J, Erickson JL, Herr R, 
            Ferik F, Kretschmer C, Berner T, Keilwagen J, Marillonnet S, Bonas U
  TITLE     Highly efficient multiplex editing: One-shot generation of 8x 
            Nicotiana benthamiana and 12x Arabidopsis mutants.
  JOURNAL   Plant J. 2021 Feb 12. doi: 10.1111/tpj.15197.
  PUBMED    33577114
REFERENCE   2  (bases 1 to 3302)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2021 Feb 12. doi: 10.1111/tpj.15197."
COMMENT     Sequence Label: pJOG1334
FEATURES             Location/Qualifiers
     source          1..3302
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     188..207
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     441..458
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1627..1645)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1713..1817
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2039..2830
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(3001..3302,1..287)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
       61 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
      121 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
      181 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
      241 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
      301 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
      361 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
      421 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
      481 gcaaaccgcc tctccccgcg cgttggccga ttcattaatc actctgtggt ctcaggagta
      541 cagtctacaa ggatgcacct tgcatggatc ctctaaagtc caattagaat ccaatacata
      601 acattgttaa aatagcccaa tccagcatca tattatagaa aggcccaaaa tagctcccaa
      661 atgtagccca ttaggcccac aacagaaaaa ccctccaatt cctgactaag cccatttact
      721 agcccatatt tttagtagga aacatataag taatgtatta aatttttctc atataataac
      781 aacaaaattt tggaaaaatt atatacacac acattttatt ttacataatc tcttaaattt
      841 tcaatcattt ttaaaaaatc aaaaatattc tctcaaattc attgctctac tcttgttaat
      901 atatttttat tccttctcga tctctggtat atcttctctt tatgtatccg aatgtctgct
      961 aatgtatctg aaagtccaat gatgtatttg aatgtcctat tctttttcta atccatccct
     1021 cccctctcga tacaccttca cttctgtatt tgaatgtatt cgaaattcat aaatttttta
     1081 aaaaaaatta taattcaaaa aagagaaaca atatgaaatt tattaaaatt atacttaaaa
     1141 ttttaacgaa gtacatgaat aattcaatag tttaattctc aacagttcat taaaattgtg
     1201 aagattcaaa gtcagagaga tccaaatgca cacatattaa ttcacgatga ccaaagttta
     1261 aatttattta tactcaaggg actaagagtg ttaattatta ttttcccttt gaagtgtcga
     1321 aaatccctca aaaaataaca aagaaacaga gaggcagaga aaaagccaaa atcgtaaaaa
     1381 cagactcttt actttcaaac tcatctctct ttctgctaaa tccatttttt tcatgcaaac
     1441 tttctcatca acatctgtca ctcttctcca cgcgtccatt ctcctcttcc tttatattca
     1501 ctcccacctc cttccccttt tttctcatcc tcaattcatt ttatttttat taaataaatt
     1561 caaattcgac cttctccacc accgtccaat gtgagaccac gaagtggctc ttcagtggac
     1621 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     1681 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     1741 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     1801 attgaaaaag gaagagtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg
     1861 gtggtaacgg cgcagtggcg gttttcatgg cttgttatga ctgttttttt ttggggtaca
     1921 gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt
     1981 atggagcagc aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt taaacatcat
     2041 gagggaagcg gtgatcgccg aagtatcgac tcaactatca gaggtagttg gcgtcatcga
     2101 gcgccatctc gaaccgacgt tgctggccgt acatttgtac ggctccgcag tggatggcgg
     2161 cctgaagcca cacagcgata ttgatttgct ggttacggtg accgtaaggc ttgatgaaac
     2221 aacgcggcga gctttgatca acgacctttt ggaaacttcg gcttcccctg gagagagcga
     2281 gattctccgc gctgtagaag tcaccattgt tgtgcacgac gacatcattc cgtggcgtta
     2341 tccagctaag cgcgaactgc aatttggaga atggcagcgc aatgacattc ttgcaggtat
     2401 cttcgagcca gccacgatcg acattgatct ggctatcttg ctgacaaaag caagagaaca
     2461 tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt gatccggttc ctgaacagga
     2521 tctatttgag gcgctaaatg aaaccttaac gctatggaac tcgccgcccg actgggctgg
     2581 cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa
     2641 aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg cccagtatca
     2701 gcccgtcata cttgaagcta gacaggctta tcttggacaa gaagaagatc gcttggcctc
     2761 gcgcgcagat cagttggaag aatttgtcca ttacgtaaaa ggcgagatca ccaaggtagt
     2821 cggcaaataa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     2881 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     2941 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     3001 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     3061 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     3121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     3181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     3241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     3301 gg
//
