LOCUS       sequence-333334-        4152 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 module (3U+Ter) for further assemblies based on the Modular 
            Cloning system. 3'UTR and flanking sequence of a Solanum 
            lycopersicum Oleosin gene, arbitrarily called OLE2 
            (Sollyc03g112440).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4152)
  AUTHORS   Stuttmann J, Barthel K, Martin P, Ordon J, Erickson JL, Herr R, 
            Ferik F, Kretschmer C, Berner T, Keilwagen J, Marillonnet S, Bonas U
  TITLE     Highly efficient multiplex editing: One-shot generation of 8x 
            Nicotiana benthamiana and 12x Arabidopsis mutants.
  JOURNAL   Plant J. 2021 Feb 12. doi: 10.1111/tpj.15197.
  PUBMED    33577114
REFERENCE   2  (bases 1 to 4152)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2021 Feb 12. doi: 10.1111/tpj.15197."
COMMENT     Sequence Label: pJOG1336
FEATURES             Location/Qualifiers
     source          1..4152
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     83..102
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     336..353
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(2372..2390)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2458..2562
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2784..3575
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(3746..4152,1..182)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
       61 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      121 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      181 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      241 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      301 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
      361 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatcactct
      421 gtggtctcag cttgatttgt taaaatctgg agtactcttt ttaaagttct gtgttttgtt
      481 aatcataatt taatatatgt gtatgtattt atcttttaag tgcttaatgt tgcagcctct
      541 tgattttcta gtactctaat aaaatgcgtg agcctttttg tttttggtat cttcttttcg
      601 ttgaatgtcg atttaatgac taacatggtg gaattttgga gttatgaaat gcactctttt
      661 aatcaactaa cattcacaat ataaattagg tccattaatg tattaactaa tttttcatat
      721 cataaatcat ttttatatat actactattt tttaaataca atatgttact acgaaccaat
      781 atttgaagaa atattccgat cttcatattc tatttcttca agtaacttga tcaaaatgct
      841 cagacctaaa agcacaaaaa cttaacagga ttaaaacctt cgttaagtgc ttatcgttat
      901 gcttggtgct ctttgccaaa tgtgtaattg cgtgggtact tgatggttcc cttgctatgg
      961 catatatgac ttggtgatag cattgaagta ttgattgttc aaatggatga ttagaaagct
     1021 atttggagac aaacaatgag ttcaaatacc attaaacaat tcaaaaaaac attattatac
     1081 gctagtgatt cttaaaccat aatgtctttt tttttccttt taaattctta atgttgctca
     1141 gataaatgat tttacttcca cattcttgga tctcctttgt atatcctttt catatttgat
     1201 aggttctgct tgagtcgagg attgataggt tctgcttgag tcgaggatat cgaaaattac
     1261 ctgtttaagg ttgcatatag accaccctct ccaaacctcg tacgtaattg ttgcttgaat
     1321 ctccaggagt aactaccttc atattgttta ttattttcag tttgtgttgc aaactactga
     1381 agtttattcc aacgggaaaa tatgtgaatc ctcttttagt cttttagaaa aggttaaaaa
     1441 ggcattaaaa cataattgta tcatttcatt tagactaaat tagcatctaa atagacattt
     1501 aaacttactt aagattaaat aagtggatac ataaatgaga ttttagtgga gagttgtttc
     1561 tcccttttaa cagaaattac aatgcacaat tcaaacagta aaaacttcta atgggaaacg
     1621 acgaaagaca aattcaaaaa ttctaaacat tcttctacac aatggagata ttaagtactt
     1681 aatagaataa tcgaggcgta aacaaagaaa gagtcaacaa taagataagt gttcaattgt
     1741 caagaaatat tatcttcaca catcgctcat caatatattg atgaaggatg caaatcttgg
     1801 cagaaaaaat gcactctttg gaactcatta tccaccaaag tttcaaacca tatgccaaat
     1861 aatgttagag cacttcttaa ttattaaaag aagtgctatt ttacatctcc aatagttcaa
     1921 acgtatcaga agcacacaaa aaagaacatc ttcaatgtcg ctagcaacac atggaggaaa
     1981 aggaattcaa tgaagaaaaa ttatgcacag gaaagtcctt tgccatgaag agcaaccagc
     2041 acaaaaataa agggaagtaa tcaaatccaa tgacaagata ttctaggcac aacaaacata
     2101 atgtttaggt ggggattgcc aatagaacat aaggagaact cttcaaattc ctatccctac
     2161 ttggaaaatg ttccccaagt tgatgttttt ttcgtctatc tgatagtatt ttcagcttcc
     2221 cggaccaaaa ataaaagaga aggaaataaa aaggggcccc ccgtttgctt gaaataccat
     2281 aatcaaggtt tcacatgctc cctaatgaac ttctccagct gagaaatctc accgcttgag
     2341 accacgaagt ggctcttcag tggacgaaag ggcctcgtga tacgcctatt tttataggtt
     2401 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
     2461 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     2521 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgcgctc acgcaactgg
     2581 tccagaacct tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttgt
     2641 tatgactgtt tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt
     2701 acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc
     2761 gccctaaaac aaagttaaac atcatgaggg aagcggtgat cgccgaagta tcgactcaac
     2821 tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt
     2881 tgtacggctc cgcagtggat ggcggcctga agccacacag cgatattgat ttgctggtta
     2941 cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa
     3001 cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc
     3061 acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc
     3121 agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta
     3181 tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac
     3241 tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat
     3301 ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca
     3361 tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa
     3421 tggagcgcct gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg
     3481 gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccattacg
     3541 taaaaggcga gatcaccaag gtagtcggca aataactgtc agaccaagtt tactcatata
     3601 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     3661 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     3721 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     3781 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     3841 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     3901 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     3961 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     4021 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     4081 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     4141 gagaaagcgc ca
//
