LOCUS       sequence-333341-       10292 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10292)
  AUTHORS   Zhu Q, Pao GM, Huynh AM, Suh H, Tonnu N, Nederlof PM, Gage FH, Verma
            IM
  TITLE     BRCA1 tumour suppression occurs via heterochromatin-mediated 
            silencing.
  JOURNAL   Nature. 2011 Sep 7;477(7363):179-84. doi: 10.1038/nature10371.
  PUBMED    21901007
REFERENCE   2  (bases 1 to 10292)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2011-09-7- 7"; volume: "477"; issue: "7363"; pages:
            "179-84"
COMMENT     Sequence Label: p156RRL-EF1a-GFPU3H1MajSat
FEATURES             Location/Qualifiers
     source          1..10292
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     126..145
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(364..468)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     536..554
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(592..614)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     714..733
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     947..966
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        1256..1274
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1256..1273
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     LTR             1299..1479
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     protein_bind    1499..1532
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        complement(2029..2243)
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     complement(2089..2108)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    2272..2290
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2314..2332
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2356..2374
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2398..2416
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2440..2458
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    2626..3214
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             2732..2743
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     3141..3161
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(join(3289..3999,4000..4002,4003..4005))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(3289..3999,4000..4002,4003..4005))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3331..3352)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     3680..3699
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3939..3960
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      4002..4011
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    4096..4213
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        complement(4261..5439)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4270..5208
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(4293..4313)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             complement(5824..5868)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6053..6286
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    6779..6904
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             6951..7584
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    7752..7886
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7776..7795)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7830..7849
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(8311..8331)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          8461..8526
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     promoter        complement(9660..9989)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9674..9809
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9974..9994
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     complement(10064..10081)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
       61 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
      121 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
      181 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
      241 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
      301 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
      361 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
      421 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
      481 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
      541 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca
      601 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
      661 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc
      721 agagcagatt gtactgagag tgcaccattc gacgctctcc cttatgcgac tcctgcatta
      781 ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat
      841 gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga
      901 aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga
      961 tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt
     1021 agaggatctg gctagcgatg accctgctga ttggttcgct gaccatttcc gggtgcggga
     1081 cggcgttacc agaaactcag aaggttcgtc caaccaaacc gactctgacg gcagtttacg
     1141 agagagatga tagggtctgc ttcagtaagc cagatgctac acaattaggc ttgtacatat
     1201 tgtcgttaga acgcggctac aattaataca taaccttatg tatcatacac atacgattta
     1261 ggtgacacta tagaatacac ggaattaatt ctagatgctg ctagagattt tccacactga
     1321 ctaaaagggt ctgagggatc tctagttacc agagtcacac aacagacggg cacacactac
     1381 ttgaagcact caaggcaagc tttattgagg cttaagcagt gggttcccta gttagccaga
     1441 gagctcccag gctcagatct ggtctaacca gagagaccca gtacaagcaa aaagcagcat
     1501 aacttcgtat aatgtatgct atacgaagtt attcccatgc gacggtatcg atttcacgtc
     1561 ctaaagtgtg tatttctcat tttccgtgaa tttcagtttt ctcgccatat tccaggtcct
     1621 tcagtgtgca tttctcattt ttcacatttt ttagtgattt tgtcattttt caagtcgtca
     1681 agtggatgtt tctcattttc catgattttc agttttcttg ccattttcca cgtcctagag
     1741 tggacatttc taaattttcc acctttttca gttatcctcg ccatagctag attcacgtcc
     1801 taaagtgtgt atttctcatt ttccgtgatt ttcagttttc tcgccatatt ccagctccta
     1861 cagtgtgcat ttctcatttt tcacgttttt cagggatttt gtcatttttc aagtcgtcaa
     1921 gtggatgttt ctcattttcc atgattttca gttttcttgc catattccac gtcctagagt
     1981 ggacatttct aaattttcca cctttttcag ttatcctcgc cataacgcgt ggtctcatac
     2041 agaacttata agattcccaa atccaaagac atttcacgtt tatggtgatt tcccagaaca
     2101 catagcgaca tgcaaatatt gcagggcgcc actcccctgt ccctcacagc catcttcctg
     2161 ccagggcgca cgcgcgctgg gtgttcccgc ctagtgacac tgggcccgcg attccttgga
     2221 gcgggttgat gacgtcagcg ttcgaattcc tgcagccccg actttcactt ttctctatca
     2281 ctgataggga gtggtaaact cgactttcac ttttctctat cactgatagg gagtggtaaa
     2341 ctcgactttc acttttctct atcactgata gggagtggta aactcgactt tcacttttct
     2401 ctatcactga tagggagtgg taaactcgac tttcactttt ctctatcact gatagggagt
     2461 ggtaaactcg agggggatcc actagcatgg tgaattgatc ccatcttgtc ttcgttggga
     2521 gtgaattagc ccttccagtc cccccttttc ttttaaaaag tggctaagat ctacagctgc
     2581 cttgtaagtc attggtctta aaggtacctg gccatacgta ccgatgcggg gaggcggccc
     2641 aaagggagat ccgactcgtc tgagggcgaa ggcgaagacg cggaagaggc cgcagagccg
     2701 gcagcaggcc gcgggaagga aggtccgctg gattgagggc cgaagggacg tagcagaagg
     2761 acgtcccgcg cagaatccag gtggcaacac aggcgagcag ccatggaaag gacgtcagct
     2821 tccccgacaa caccacggaa ttgtcagtgc ccaacagccg agcccctgtc cagcagcggg
     2881 caaggcaggc ggcgatgagt tccgccgtgg caatagggag ggggaaagcg aaagtcccgg
     2941 aaaggagctg acaggtggtg gcaatgcccc aaccagtggg ggttgcgtca gcaaacacag
     3001 tgcacaccac gccacgttgc ctgacaacgg gccacaactc ctcataaaga gacagcaacc
     3061 aggatttata caaggaggag aaaatgaaag ccatacggga agcaatagca tgatacaaag
     3121 gcattaaagc agcgtatcca catagcgtaa aaggagcaac atagttaaga ataccagtca
     3181 atctttcaca aattttgtaa tccagaggtt gattatcata tgactagtcc ccgaagcttg
     3241 agctcgacta gctagctagc tactagctag tcgagatctg agtccggact tgtacagctc
     3301 gtccatgccg agagtgatcc cggcggcggt cacgaactcc agcaggacca tgtgatcgcg
     3361 cttctcgttg gggtctttgc tcagggcgga ctgggtgctc aggtagtggt tgtcgggcag
     3421 cagcacgggg ccgtcgccga tgggggtgtt ctgctggtag tggtcggcga gctgcacgct
     3481 gccgtcctcg atgttgtggc ggatcttgaa gttcaccttg atgccgttct tctgcttgtc
     3541 ggccatgata tagacgttgt ggctgttgta gttgtactcc agcttgtgcc ccaggatgtt
     3601 gccgtcctcc ttgaagtcga tgcccttcag ctcgatgcgg ttcaccaggg tgtcgccctc
     3661 gaacttcacc tcggcgcggg tcttgtagtt gccgtcgtcc ttgaagaaga tggtgcgctc
     3721 ctggacgtag ccttcgggca tggcggactt gaagaagtcg tgctgcttca tgtggtcggg
     3781 gtagcggctg aagcactgca cgccgtaggt cagggtggtc acgagggtgg gccagggcac
     3841 gggcagcttg ccggtggtgc agatgaactt cagggtcagc ttgccgtagg tggcatcgcc
     3901 ctcgccctcg ccggacacgc tgaacttgtg gccgtttacg tcgccgtcca gctcgaccag
     3961 gatgggcacc accccggtga acagctcctc gcccttgctc accatggtgg cgaccggtag
     4021 cgctaggacg cgcgcgtaag cttaggcctt cgaagatccc gtagtttaaa cctcgaggct
     4081 agtctcgtga tcgataaaat tttgaatttt tgtaatttgt ttttgtaatt ctttagtttg
     4141 tatgtctgtt gctattatgt ctactattct ttcccctgca ctgtaccccc caatcccccc
     4201 ttttctttta aaagttaacc gataccgtcg agattaatta aatttaaatg tcgaaattcc
     4261 tcacgacacc tgaaatggaa gaaaaaaact ttgaaccact gtctgaggct tgagaatgaa
     4321 ccaagatcca aactcaaaaa gggcaaattc caaggagaat tacatcaagt gccaagctgg
     4381 cctaacttca gtctccaccc actcagtgtg gggaaactcc atcgcataaa acccctcccc
     4441 ccaacctaaa gacgacgtac tccaaaagct cgagaactaa tcgaggtgcc tggacggcgc
     4501 ccggtactcc gtggagtcac atgaagcgac ggctgaggac ggaaaggccc ttttcctttg
     4561 tgtgggtgac tcacccgccc gctctcccga gcgccgcgtc ctccattttg agctccctgc
     4621 agcagggccg ggaagcggcc atctttccgc tcacgcaact ggtgccgacc gggccagcct
     4681 tgccgcccag ggcggggcga tacacggcgg cgcgaggcca ggcaccagag caggccggcc
     4741 agcttgagac tacccccgtc cgattctcgg tggccgcgct cgcaggcccc gcctcgccga
     4801 acatgtgcgc tgggacgcac gggccccgtc gccgcccgcg gccccaaaaa ccgaaatacc
     4861 agtgtgcaga tcttggcccg catttacaag actatcttgc cagaaaaaaa gcgtcgcagc
     4921 aggtcatcaa aaattttaaa tggctagaga cttatcgaaa gcagcgagac aggcgcgaag
     4981 gtgccaccag attcgcacgc ggcggcccca gcgcccaggc caggcctcaa ctcaagcacg
     5041 aggcgaaggg gctccttaag cgcaaggcct cgaactctcc cacccacttc caacccgaag
     5101 ctcgggatca agaatcacgt actgcagcca ggtggaagta attcaaggca cgcaagggcc
     5161 ataacccgta aagaggccag gcccgcggga accacacacg gcacttacct gtgttctggc
     5221 ggcaaacccg ttgcgaaaaa gaacgttcac ggcgactact gcacttatat acggttctcc
     5281 cccaccctcg ggaaaaaggc ggagccagta cacgacatca ctttcccagt ttaccccgcg
     5341 ccaccttctc taggcaccgg ttcaattgcc gacccctccc cccaacttct cggggactgt
     5401 gggcgatgtg cgctctgccc actgacgggc accggagcca attcccactc ctttcaagac
     5461 ctagaaggtc cattagctgc aaagattcct ctctgtttaa aactttatcc atctttgcaa
     5521 agcttatcgt cgacatcgcg atggtcgagg ctagtctcgt gatcgatacc gtcgagatcc
     5581 gttcactaat cgaatggatc tgtctctgtc tctctctcca ccttcttctt ctattccttc
     5641 gggcctgtcg ggtcccctcg gggttgggag gtgggtctga aacgataatg gtgaatatcc
     5701 ctgcctaact ctattcacta tagaaagtac agcaaaaact attcttaaac ctaccaagcc
     5761 tcctactatc attatgaata attttatata ccacagccaa tttgttatgt taaaccaatt
     5821 ccacaaactt gcccatttat ctaattccaa taattcttgt tcattctttt cttgctggtt
     5881 ttgcgattct tcaattaagg agtgtattaa gcttgtgtaa ttgttaattt ctctgtccca
     5941 ctccatccag gtcgtgtgat tccaaatctg ttccagagat ttattactcc aactagcatt
     6001 ccaaggcaca gcagtggtgc aaatgagttt tccagagcaa ccccaaatcc ccaggagctg
     6061 ttgatccttt aggtatcttt ccacagccag gattcttgcc tggagctgct tgatgcccca
     6121 gactgtgagt tgcaacagat gctgttgcgc ctcaatagcc ctcagcaaat tgttctgctg
     6181 ctgcactata ccagacaata attgtctggc ctgtaccgtc agcgtcattg acgctgcgcc
     6241 catagtgctt cctgctgctc ccaagaaccc aaggaacaaa gctcctattc ccactgctct
     6301 tttttctctc tgcaccactc ttctctttgc cttggtgggt gctactccta atggttcaat
     6361 ttttactact ttatatttat ataattcact tctccaattg tccctcatat ctcctcctcc
     6421 aggtctgaag atcagcggcc gcttgctgtg cggtggtctt acttttgttt tgctcttcct
     6481 ctatcttgtc taaagcttcc ttggtgtctt ttatctctat cctttgatgc acacaataga
     6541 gggttgctac tgtattatat aatgatctaa gttcttctga tcctgtctga agggatggtt
     6601 gtagctgtcc cagtatttgt ctacagcctt ctgatgtttc taacaggcca ggattaactg
     6661 cgaatcgttc tagctccctg cttgcccata ctatatgttt taatttatat tttttctttc
     6721 cccctggcct taaccgaatt ttttcccatc gcgatctaat tctcccccgc ttaatactga
     6781 cgctctcgca cccatctctc tccttctagc ctccgctagt caaaattttt ggcgtactca
     6841 ccagtcgccg cccctcgcct cttgccgtgc gcgcttcagc aagccgagtc ctgcgtcgag
     6901 agagctcctc tggtttccct ttcgctttca agtccctgtt cgggcgccac tgctagagat
     6961 tttccacact gactaaaagg gtctgaggga tctctagtta ccagagtcac acaacagacg
     7021 ggcacacact acttgaagca ctcaaggcaa gctttattga ggcttaagca gtgggttccc
     7081 tagttagcca gagagctccc aggctcagat ctggtctaac cagagagacc cagtacaggc
     7141 aaaaagcagc tgcttatatg caggatctga gggctcgcca ctccccagtc ccgcccaggc
     7201 cacgcctccc tggaaagtcc ccagcggaaa gtcccttgta gcaagctcga tatcagcagt
     7261 tcttgaagta ctccggatgc agctctcggg ccacgtgatg aaatgctagg cggctgtcaa
     7321 acctccactc taacacttct ctctccgggt catccatccc atgcaggctc acagggtgta
     7381 acaagctggt gttctctcct ttattggcct cttctacctt atctggctca actggtacta
     7441 gcttgtagca ccatccaaag gtcagtggat atctgacccc tggccctggt gtgtagttct
     7501 gctaatcagg gaagtagcct tgtgtgtggt agatccacag atcaaggata tcttgtcttc
     7561 tttgggagtg aattagccct tccaactact aagtttgtag tacatattta acaaatacaa
     7621 tttctttaaa atgaaaataa ttcagaggaa tcacaggttt agagtaaatg aaaccacagg
     7681 taattggcag tggtaatagg gtatggggtg ggaagtttgg gatgattttg gttagcttga
     7741 gttatccagt tgatccagac atgataagat acattgatga gtttggacaa accacaacta
     7801 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
     7861 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg
     7921 ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg
     7981 ctgattatga tctctagtca aggcactata catcaaatat tccttattaa cccctttaca
     8041 aattaaaaag ctaaaggtac acaatttttg agcatagtta ttaatagcag acactctatg
     8101 cctgtgtgga gtaagaaaaa acagtatgtt atgattataa ctgttatgcc tacttataaa
     8161 ggttacagaa tatttttcca taattttctt gtatagcagt gcagcttttt cctttgtggt
     8221 gtaaatagca aagcaagcaa gagttctatt actaaacaca gcatgactca aaaaacttag
     8281 caattctgaa ggaaagtcct tggggtcttc tacctttctc ttcttttttg gaggagtaga
     8341 atgttgagag tcagcagtag cctcatcatc actagatggc atttcttctg agcaaaacag
     8401 gttttcctca ttaaaggcat tccaccactg ctcccattca tcagttccat aggttggaat
     8461 ctaaaataca caaacaatta gaatcagtag tttaacacat tatacactta aaaattttat
     8521 atttacctta gagctttaaa tctctgtagg tagtttgtcc aattatgtca caccacagaa
     8581 gtaaggttcc ttcacaaaga tccggggccc actcataaat ccagttgccg ccacggtagc
     8641 caatcaccgt atcgtataaa tcatcgtcgg tacgttcggc atcgctcatc acaatacgtg
     8701 cctggacgtc gaggatttcg cgtgggtcaa tgccgcgcca gatccacatc agacggttaa
     8761 tcatgcgata ccagtgaggg atggttttac catcaagggc cgactgcaca ggcggttgtg
     8821 cgccgtgatt aaagcggcgg actagcgtcg aggtttcagg atgtttaaag cggggtttga
     8881 acagggtttc gctcaggttt gcctgtgtca tggatgcagc ctccagaata cttactggaa
     8941 actattgtaa cccgcctgaa gttaaaaaga acaacgcccg gcagtgccag gcgttgaaaa
     9001 gattagcgac cggagattgg cgggacgaat acgacgccca tatcccacgg ctgttcaatc
     9061 caggtatctt gcgggatatc aacaacatag tcatcaacca gcggacgacc agccggtttt
     9121 gcgaagatgg tgacaaagtg cgcttttgga tacatttcac gaatcgcaac cgcagtacca
     9181 ccggtatcca ccaggtcatc aataacgatg aagccttcgc catcgccttc tgcgcgtttc
     9241 agcactttaa gctcgcgctg gttgtcgtga tcgtagctgg aaatacaaac ggtatcgaca
     9301 tgacgaatac ccagttcacg cgccagtaac gcacccggta cagaccgcca cggcttacgg
     9361 caataatgcc tttccattgt tcagaaggca tcagtcggct tgcgagttta cgtgcatgga
     9421 tctgcaacat gtcccaggtg acgatgtatt tttcgctcat gtgaagtgtc ccagcctgtt
     9481 tatctacggc ttaaaaagtg ttcgagggga aaataggttg cgcgagatta tagagatctg
     9541 gcgcactaaa aaccagtatt tcacatgagt ccgcgtcttt ttacgcactg cctctccctg
     9601 acgcgggata aagtggtatt ctcaaacata tctcgcaagc ctgtcttgtg tccaagcttt
     9661 ttgcaaaagc ctaggcctcc aaaaaagcct cctcactact tctggaatag ctcagaggcc
     9721 gaggcggcct cggcctctgc ataaataaaa aaaattagtc agccatgggg cggagaatgg
     9781 gcggaactgg gcggagttag gggcgggatg ggcggagtta ggggcgggac tatggttgct
     9841 gactaattga gatgcatgct ttgcatactt ctgcctgctg gggagcctgg ggactttcca
     9901 cacctggttg ctgactaatt gagatgcatg ctttgcatac ttctgcctgc tggggagcct
     9961 ggggactttc cacaccctaa ctgacacaca ttccacagct gcattaatga atcggccaac
    10021 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
    10081 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
    10141 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
    10201 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
    10261 agcatcacaa aaatcgacgc tcaagtcaga gg
//
