LOCUS       sequence-33335-e         528 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Kodama Y, Hu CD
  TITLE     An improved bimolecular fluorescence complementation assay with a 
            high signal-to-noise ratio.
  JOURNAL   Biotechniques. 2010 Nov . 49(5):793-805.
  PUBMED    21091444
REFERENCE   2  (bases 1 to 528)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biotechniques. 2010 Nov . 49(5):793-805."
COMMENT     Sequence Label: pBiFC-VN155(I152L)
FEATURES             Location/Qualifiers
     source          1..528
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     45..64
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     304..325
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 tgcggttcac cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg tagttgccgt
       61 cgtccttgaa gaagatggtg cgctcctgga cgtagccttc gggcatggcg gacttgaaga
      121 agtcgtgctg cttcatgtgg tcggggtagc gggcgaagca ctgcaggccg tagcccaggg
      181 tggtcacgag ggtgggccag ggcacgggca gcttgccggt ggtgcagatc agcttcaggg
      241 tcagcttgcc gtaggtggca tcgccctcgc cctcgccgga cacgctgaac ttgtggccgt
      301 ttacgtcgcc gtccagctcg accaggatgg gcaccacccc ggtgaacagc tcctcgccct
      361 tgctcaccat actcccgcca cctccgctcc cgccacctcc ggtacctcga gagatctcgg
      421 tcgaccgaat tcgggcctcc atggccataa gcaggtcctc ctctgagatc agcttctgca
      481 ttgatgccat ggtgggcccg cgggtacaat tccgcagctt ttagagca
//
