LOCUS       sequence-333354-         399 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express Cys-DogCatcher protein in bacterial cytoplasm. The cysteine 
            enables convenient coupling to maleimide-dye or thiol-reactive 
            resin..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Keeble AH, Yadav VK, Ferla MP, Bauer CC, Chuntharpursat-Bon E, Huang
            J, Bon RS, Howarth M
  TITLE     DogCatcher allows loop-friendly protein-protein ligation.
  JOURNAL   Cell Chem Biol. 2021 Jul 28. pii: S2451-9456(21)00315-9. doi: 
            10.1016/j.chembiol.2021.07.005.
  PUBMED    34324879
REFERENCE   2  (bases 1 to 399)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Chem 
            Biol. 2021 Jul 28. pii: S2451-9456(21)00315-9. doi: 
            10.1016/j.chembiol.2021.07.005."
COMMENT     Sequence Label: pDEST14-Cys-DogCatcher
FEATURES             Location/Qualifiers
     source          1..399
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             52..72
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             85..396
                     /codon_start=1
                     /product="engineered fragment of the RrgA adhesin of 
                     Streptococcus pneumoniae"
                     /label=DogCatcher
                     /note="forms a covalent bond with an intra-domain protein 
                     loop containing the DogTag peptide (Keeble et al., 2022)"
                     /translation="KLGEIEFIKVDKTDKKPLRGAVFSLQKQHPDYPDIYGAIDQNGTY
                     QDVRTGEDGKLTFTNLSDGKYRLIENSEPPGYKPVQNKPIVSFRIVDGEVRDVTSIVPQ
                     "
ORIGIN
        1 atgtcgtact accatcacca tcaccatcac gattacgaca tcccaacgac cgaaaacctg
       61 tattttcagg gcggttgcgg tatgaaactg ggcgagattg aatttattaa agtggacaaa
      121 accgataaaa agccgctgcg tggtgccgtg tttagcctgc agaaacagca tcccgactat
      181 cccgatatct atggcgcgat tgatcagaat gggacctatc aagatgtgcg taccggcgaa
      241 gatggtaaac tgacctttac gaatctgagc gatggcaaat atcgcctgat tgaaaatagc
      301 gaacccccgg gctataaacc ggtgcagaat aagccgattg tgagctttcg tattgtggat
      361 ggcgaagtgc gtgatgtgac cagtattgtg ccgcagtaa
//
