LOCUS       sequence-33336-e         929 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Kodama Y, Hu CD
  TITLE     An improved bimolecular fluorescence complementation assay with a 
            high signal-to-noise ratio.
  JOURNAL   Biotechniques. 2010 Nov . 49(5):793-805.
  PUBMED    21091444
REFERENCE   2  (bases 1 to 929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biotechniques. 2010 Nov . 49(5):793-805."
COMMENT     Sequence Label: pBiFC-bJunVN155(I152L)
FEATURES             Location/Qualifiers
     source          1..929
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(14..472,473..475,476..478))
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYLTA"
     primer_bind     153..172
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     412..433
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     intron          827..923
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
ORIGIN
        1 ccgcggccgc tcaggcggtg agatagacgt tgtggctgtt gtagttgtac tccagcttgt
       61 gccccaggat gttgccgtcc tccttgaagt cgatgccctt cagctcgatg cggttcacca
      121 gggtgtcgcc ctcgaacttc acctcggcgc gggtcttgta gttgccgtcg tccttgaaga
      181 agatggtgcg ctcctggacg tagccttcgg gcatggcgga cttgaagaag tcgtgctgct
      241 tcatgtggtc ggggtagcgg gcgaagcact gcaggccgta gcccagggtg gtcacgaggg
      301 tgggccaggg cacgggcagc ttgccggtgg tgcagatcag cttcagggtc agcttgccgt
      361 aggtggcatc gccctcgccc tcgccggaca cgctgaactt gtggccgttt acgtcgccgt
      421 ccagctcgac caggatgggc accaccccgg tgaacagctc ctcgcccttg ctcaccatac
      481 tcccgccacc tccgctcccg ccacctccgg taccgtggtt catgactttc tgtttaagct
      541 gtgccacctg ttccctgagc atgttggccg tggacgccag ctcggagttt tgcgctttca
      601 aggttttcac tttttcctct agccgggcga tccgctccag cttccttttc cggcacttgg
      661 aggcagcgat gcggttcctc atgcgcttcc tctccgcctt ccgaattcgg gcctccatgg
      721 ccataagcag gtcctcctct gagatcagct tctgcattga tgccatggtg ggcccgcggg
      781 tacaattccg cagcttttag agcagaagta acacttccgt acaggcctag aagtaaaggc
      841 aacatccact gaggagcagt tctttgattt gcaccaccac cggatccggg acctgaaata
      901 aaagacaaaa agactaaact taccagtta
//
