LOCUS       sequence-333363-        4393 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4393)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 4393)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_5277:Dendra
FEATURES             Location/Qualifiers
     source          1..4393
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(647..670)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     rep_origin      1689..2277
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2178..2197
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 caggggcaat ggatcagaga tgatctgctc tgcctgtggc cccgctgccg caaaggcaaa
       61 tggatgggcg ctgcgcttta catttggcag gcgccagaat gtgtcagaga caactccaag
      121 gtccggtgta acgggcgacg tggcaggatc gaacggctcg tcgtccagac ctgaccacga
      181 gggcatgacg agcgtccctc ccggacccag cgtagcacgc agggcctcga tcagtccaag
      241 tggcccatct tcgaggggcc ggacgctacg gaaggagctg tggaccagca gcacaccgcc
      301 gggggtaacc ccaaggttga gaagctgacc gatgagctcg gcttttcgcc attcgtattg
      361 cacgacattg cactccaccg ctgatgacat cagtcgatca tagcacgatc aacggcactg
      421 ttgcaaatag tcggtggtga taaacttatc atcccctttt gctgatggag ctgcacatga
      481 acccattcaa aggccggcat tttcagcgtg acatcattct gtgggccgta cgctggtact
      541 gcaaatacgg catcagttac cgtgagctgc aggaattcga tcgtacgcta gttaacaaaa
      601 aacccctcaa gacccgttta gaggccccaa ggggttattt aaatcacttg tcgtcgtcgt
      661 ccttgtagtc ctggaaatac aggttctccc cgccgccgcc ttcgaaccag acctgcgacg
      721 ggagcgggct atagcgtgcc acagcgtgct cgtacaactt gaccttgttg tagtccgaat
      781 cgttccccag gatttcgatg cggtggtcca cgaagtgcgc gtcgggcagc ttctcaacct
      841 tggggttacc cccggcggtg tgctgctggt ccacagctcc ttccgtagcg tccggcccct
      901 cgaagatggg ccacttggac tgatcgaggc cctgcgtgct acgctgggtc cgggagggac
      961 gctcgtcatg ccctcgtggt caggtctgga cgacgagccg ttcgatcctg ccacgtcgcc
     1021 cgttacaccg gaccttggag ttgtctctga cacattctgg cgcctgccaa atgtaaagcg
     1081 cagcgcccat ccatttgcct ttgcggcagc ggggccacag gcagagcaga tcatctctga
     1141 tccattgccc ctgccacctt actcgcctgc aagcccggtc gcccgtgtcc atgaactcga
     1201 tgggcaggta cttctcctcg gcgtgggaca cgatgccaac acgacgctgc atcttgccga
     1261 gttgatggca aaggttccct atggggtgcc gagacactgc accattcttc aggatggcaa
     1321 gttggtacgc gtcgattatc tcgagaatga ccactgctgt gagcgctttg ccttggcgga
     1381 caggtggctc aaggagaaga gccttcagaa ggaaggtcca gtcggtcatg cctttgctcg
     1441 gttgatccgc tcccgcgaca ttgtggcgac agccctgggt caactgggcc gagatccgtt
     1501 gatcttcctg catccgccag aggcgggatg cgaagaatgc gatgccgctc gccagtcgat
     1561 tggctgagct catgagcgga gaacgagatg acgttggagg ggcaaggtcg cgctgattgc
     1621 tggggcaaca cgggggatcc actagttcca tgagcgtcag accccgtaga aaagatcaaa
     1681 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     1741 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     1801 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
     1861 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     1921 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     1981 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     2041 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     2101 cccgagggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     2161 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     2221 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     2281 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     2341 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     2401 accgctcgcc gcagccgaac gaccgagcgc aacgcgtgcg gccgcggtac ccggggatcc
     2461 tctagagtcg accaccaagg gcaccatctc tgcttgggcc accccgttgg ccgcagccag
     2521 ctcgctgaga gccgtgaacg acagggcgaa cgccagcccg ccgacggcga gggttccgac
     2581 cgctgcaact cccggtgcaa ccttgtcccg gtctattctc ttcactgcac cagctccaat
     2641 ctggtgtgaa tgcccctcgt ctgttcgcgc aggcgggggg ctctattcgt ttgtcagcat
     2701 cgaaagtagc cagatcaggg atgcgttgca accgcgtatg cccaggtcag aagagtcgca
     2761 caagagttgc agacccctgg aaagaaaaat ggccagaggg cgaaaacacc ctctgaccag
     2821 cggagcgggc gacgggaatc gaacccgcgt agctagtttg gaagaatggg tgtctgccga
     2881 ccacatatgg gccggtcaag ataggttttt accccctctc ggctgcatcc tctaagtgga
     2941 aagaaattgc aggtcgtaga agcgcgttga agcctgagag ttgcacagga gttgcaaccc
     3001 ggtagccttg ttcacgacga gaggagacct agttggcacg tcgcggatgg ggatcgctga
     3061 agactcagcg cagcgggagg atccaagcct catacgtcaa cccgcaggac ggtgtgaggt
     3121 actacgcgct gcagacctac gacaacaaga tggacgccga agcctggctc gcgggcgaga
     3181 agcggctcat cgagatggag acctggaccc ctccacagga ccgggcgaag aaggcagccg
     3241 ccagcgccat cctaggagtc gtgtgaccga gacgggccat cagctccttg gtcgtcgcac
     3301 ctgcctgagc ggcgaacgta gcgccgacag cgcggaggtc gtggatgcgg agttccggcc
     3361 gaccgatctt ggcgtagcca cgcttcagcg acttggtgaa cgcggacttc gacagccggt
     3421 tgccctgcgt cgtggtcacc aggaatgcct cggggccctt gttcatcttc gtacggtcct
     3481 tcatgtgcgc tcggatcatc tccgcgacgt gaggcggaac cgtcacagga cgcttcgacc
     3541 ggacggtctt ggcgttgcca acgacgatct tgttccccac gcgggaagcg ccacggcgca
     3601 cccggagctt catcgtcatg ccgtcgtcca cgatgtcctt gcggcgaagc tcgatcagct
     3661 ctccgaaccg gaggctcgtc cacgccagga tgtatgccgc gatccggtag tgctcgaaga
     3721 tctcagcggc gacgatgtcc agctcctcag gcgtcagcgc ctctacgtcg cgctcatcgg
     3781 ctgccttctg ctcgatccgg cacgggttct ctgcgatcag cttgtcctcg accgctgtgt
     3841 tcatcaccgc ccggaggacg ttgtaggcat gccggcgggc agtcgggtgc ttcctaccca
     3901 tcccggccca ccacgcacgc accagagctg gcgtcatctc tgtgaccgcc acttcaccta
     3961 gcaccgggta gatgcggcgc tccgcgtgcc cgctgtacag atccctggtg ccgtctgcga
     4021 ggtcgcgctc cacgagccac ttccgggtgt actcctccag cgtgatggcg ctggcggctg
     4081 ccttcttcgc ccggtcctgt ggaggggtcc aggtctccat ctcgatgagc cgcttctcgc
     4141 ccgcgagcca ggcttcggcg tccatcttgt tgtcgtaggt ctgcagcgcg tagtacctca
     4201 caccgtcctg cgggttgacg tatgaggctt ggatcctccc gctgcgctga gtcttcagcg
     4261 atccccatcc gcgacgtgcc aactaggtct cctctcgtcg tgaacaaggc taccgggttg
     4321 caactcctgt gcaactctca ggcttcaacg cgcttctacg acctgcaatt tctttccact
     4381 tagaggatgc agc
//
