LOCUS       sequence-333377-       11242 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of Cas9 in Culex quinquefasciatus or other mosquitoes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11242)
  AUTHORS   Feng X, Lopez Del Amo V, Mameli E, Lee M, Bishop AL, Perrimon N, 
            Gantz VM
  TITLE     Optimized CRISPR tools and site-directed transgenesis towards gene 
            drive development in Culex quinquefasciatus mosquitoes.
  JOURNAL   Nat Commun. 2021 May 20;12(1):2960. doi: 10.1038/s41467-021-23239-0.
  PUBMED    34017003
REFERENCE   2  (bases 1 to 11242)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-05-20"; volume: "12(1):2960. doi"; pages: " 
            10.1038/s41467-021-23239-0"
COMMENT     Sequence Label: pVMG0213_Cq-vasa-Cas9
FEATURES             Location/Qualifiers
     source          1..11242
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      89..517
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(176..195)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     386..407
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             861..1655
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(915..934)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1525..1544
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             join(1673..1741,1742..2533)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1891..1910)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2704..3292
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3193..3212
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3446..3463
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3580..3601
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3616..3621,3622..3639,3640..3646)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3654..3670
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3659..3681
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3678..3694
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3678..3694
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             6183..10286
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6183..10286
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             10293..10313
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(11148..11167)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(11149..11167)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11174..11191)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11174..11190)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11183..11205)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
       61 acagttgcgc agcctgaatg gcgaatggac gcgccctgta gcggcgcatt aagcgcggcg
      121 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
      181 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
      241 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
      301 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
      361 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
      421 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
      481 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaattca gggcgcaagg
      541 gctgctaaag gaagcggaac acgtagaaag ccagtccgca gaaacggtgc tgaccccgga
      601 tgaatgtcag ctactgggct atctggacaa gggaaaacgc aagcgcaaag agaaagcagg
      661 tagcttgcag tgggcttaca tggcgatagc tagactgggc ggttttatgg acagcaagcg
      721 aaccggaatt gccagctggg gcgccctctg gtaaggttgg gaagccctgc aaagtaaact
      781 ggatggcttt cttgccgcca aggatctgat ggcgcagggg atcaagatct gatcaagaga
      841 caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg
      901 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
      961 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
     1021 ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg
     1081 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
     1141 tgggcgaagt gccggggcag gatctcctgt catcccacct tgctcctgcc gagaaagtat
     1201 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
     1261 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
     1321 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
     1381 tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc
     1441 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
     1501 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
     1561 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
     1621 tcgccttcta tcgccttctt gacgagttct tctgaattga aaaaggaaga gtatgagtat
     1681 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     1741 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     1801 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     1861 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     1921 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     1981 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     2041 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     2101 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     2161 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     2221 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     2281 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     2341 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     2401 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     2461 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     2521 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     2581 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     2641 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     2701 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     2761 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     2821 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     2881 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     2941 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     3001 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     3061 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     3121 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     3181 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     3241 acttgagcgc cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     3301 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     3361 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     3421 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     3481 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     3541 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     3601 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     3661 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc ttggtaccga
     3721 gctcggatcc actagtaacg gccgccagtg tgctggaatt cgcccttccg tgcataattc
     3781 gatttgatgc ggtagcaaac aggctgaaaa gcgggttgca aagtaaaacc tcgaagaact
     3841 gagagcaaat ttgctaccgc gacaggtacc gcgtcgggtg caattttgat ttgcttcgat
     3901 gtttgctacc cgcgctggag atgcacttag gaacgagtag gaaagacttt ttgtttacac
     3961 attggcccaa ttacactgtt ttgcttgaat tggccagtgc ctatgtgtaa acaaacagtc
     4021 tatttctatg tttcctatta ggctatttgt tgcttgatat tggctgaaaa tgttgaagct
     4081 gtaattgaac gccgaagtgc tgacatgcca acattacgtg cacgatggaa atacatgccg
     4141 ttcccctgtt tagcgtcagc aaacaggttc taggattgat ttcgatctga tcccaacaag
     4201 aatgttaaga aaatagaaat attaaaaact ctgaacgtga acgaaaaatc atagttactc
     4261 gaggcggggt tggatcccca gtcctttcga ttggttagca ataatgctac ccactaggcc
     4321 atcgcggctt ggtgagctat ggttggaatt aggaataatg ctcctagagc aagtttgctg
     4381 cgatataacc agggtattag tggagataaa atttggagtt ttttttaaaa aggtccaata
     4441 aaccaaattt tcaatttttg ctttttgggt gtttttgagt cttttgatcc cggagtatca
     4501 aaccttctta gcatggtttt gtctgtggca tggtgcttcc aacgaataaa ccaaatctcg
     4561 gagcgcggtg ttgtgtgccc tctagggtga caataaaaat aaagagagtg aaaatgttga
     4621 gcctcctcgg tccttacctg gtcacagcac ctaaaaggac ctaataaaaa tgagttgaga
     4681 ataaatcaaa taaattggac ccaaatcggt taaggtcgct ttttatcgtt gaagtttgaa
     4741 tgaaaaaaaa aaaaacgcat aaaaaacccg cttcaacgaa tcatctctgc ggtaaacttt
     4801 acgcagtagg cctggccgcg tttacgtctg tgatgtttca acgcattccg atgcaatggc
     4861 gcactacaaa tgtttatgaa tgacaagaaa gatgtcagcc ggctttctaa aggatccctt
     4921 tgaaagattg gctgcgccag gctttgatga gattagatta ggagcgtaac gttttgtaat
     4981 tcgattcctc tgaaacgaag caacatttca agcaaaaaaa aaagttaatt ggccaaagcc
     5041 aaagtgtaaa caaagagtct gtcctgctca ttgctcatgt ttaacccact gactgacgta
     5101 aacaggacag actgtttaca ctttcgattt tggcccattt actatgtttt gcttggtttt
     5161 agcaatctgt acttcgtgtt cagataaaaa aaatcgtttt ttaaagcttg gaaaggtgca
     5221 gtgtcaatgt tacaatgata actaccacaa agaagaattt taaatacttt gaatagcacg
     5281 tcccgagatg ggagttggac aaagtcagtc tttgattaga ttagatcagt gtgatttcta
     5341 catcactacg agaagcacac ccaaatttag aacacgaaat ctgtcttcta ccacaggtca
     5401 cttctagaac agtgatcgta attcccgaac gttccccgtc ccaacgcggg ccaaaatgtc
     5461 aaactgcaaa gttgtttaca aacgcaaacc cggcttttcc ttctgccctc ttcccgcact
     5521 atttccccaa aggaaataag tcgagcaaca cattccgtgc agaaagtaat tcgatatctg
     5581 cgctgcacac aagtccctta ttttaaattg tgctgtgtcg tgcagttttc ggcccgaatg
     5641 tggcgccgat gaagagtagt tgtggcgcgc gagcgagcct tcgatttggt aagtgcacac
     5701 gggaaatgtt aggactgcgg cgggggtggt tttgggtggt gcacgcacca gcagccgtga
     5761 accgcgaacg agccggcaga taaacggggg agagggagat ggaaaaaaaa gtggaaaaaa
     5821 tagcggcaaa ttagccgaag aaacggttgg ccggtggtgt gactgctgct gctgcggacc
     5881 acatctctcg cgcttgttgt cactcatccg caaacaatta atacggttga aggctggccg
     5941 gccgaggctt tttgccggag aaggcctcgc gtttcaaagt aatacaatta tgataaccga
     6001 atgctgtgcg gctttctttt cgctcgtgct cgtgccttat taacttttag tgatactttg
     6061 attttttttt cctttcttcg cgtttagccc acaaatcctc ctccccccgc gaatgcttta
     6121 cgactcatct ccgcttattt tttttctctt ttgtcgtttc ttttcagtaa gtcaacgcca
     6181 acatggataa gaagtactcg atcggtctgg atatcggtac gaactcggtg ggttgggccg
     6241 tgatcacgga tgagtacaag gtgccgtcga agaagttcaa ggtgctgggt aacacggatc
     6301 gccactcgat caagaagaac ctgatcggtg ccctgctgtt cgattcgggt gagacggccg
     6361 aggccacgcg cctgaagcgc acggcccgcc gccgctacac gcgccgcaag aaccgcatct
     6421 gctacctgca ggagatcttc tcgaacgaga tggccaaggt ggatgattcg ttcttccacc
     6481 gcctggagga gtcgttcctg gtggaggagg ataagaagca cgagcgccac ccgatcttcg
     6541 gtaacatcgt ggatgaggtg gcctaccacg agaagtaccc gacgatctac cacctgcgca
     6601 agaagctggt ggattcgacg gataaggccg atctgcgcct gatctacctg gccctggccc
     6661 acatgatcaa gttccgcggt cacttcctga tcgagggtga tctgaacccg gataactcgg
     6721 atgtggataa gctgttcatc cagctggtgc agacgtacaa ccagctgttc gaggagaacc
     6781 cgatcaacgc ctcgggtgtg gatgccaagg ccatcctgtc ggcccgcctg tcgaagtcgc
     6841 gccgcctgga gaacctgatc gcccagctgc cgggtgagaa gaagaacggt ctgttcggta
     6901 acctgatcgc cctgtcgctg ggtctgacgc cgaacttcaa gtcgaacttc gatctggccg
     6961 aggatgccaa gctgcagctg tcgaaggata cgtacgatga tgatctggat aacctgctgg
     7021 cccagatcgg tgatcagtac gccgatctgt tcctggccgc caagaacctg tcggatgcca
     7081 tcctgctgtc ggatatcctg cgcgtgaaca cggagatcac gaaggccccg ctgtcggcct
     7141 cgatgatcaa gcgctacgat gagcaccacc aggatctgac gctgctgaag gccctggtgc
     7201 gccagcagct gccggagaag tacaaggaga tcttcttcga tcagtcgaag aacggttacg
     7261 ccggttacat cgatggtggt gcctcgcagg aggagttcta caagttcatc aagccgatcc
     7321 tggagaagat ggatggtacg gaggagctgc tggtgaagct gaaccgcgag gatctgctgc
     7381 gcaagcagcg cacgttcgat aacggttcga tcccgcacca gatccacctg ggtgagctgc
     7441 acgccatcct gcgccgccag gaggatttct acccgttcct gaaggataac cgcgagaaga
     7501 tcgagaagat cctgacgttc cgcatcccgt actacgtggg tccgctggcc cgcggtaact
     7561 cgcgcttcgc ctggatgacg cgcaagtcgg aggagacgat cacgccgtgg aacttcgagg
     7621 aggtggtgga taagggtgcc tcggcccagt cgttcatcga gcgcatgacg aacttcgata
     7681 agaacctgcc gaacgagaag gtgctgccga agcactcgct gctgtacgag tacttcacgg
     7741 tgtacaacga gctgacgaag gtgaagtacg tgacggaggg tatgcgcaag ccggccttcc
     7801 tgtcgggtga gcagaagaag gccatcgtgg atctgctgtt caagacgaac cgcaaggtga
     7861 cggtgaagca gctgaaggag gattacttca agaagatcga gtgcttcgat tcggtggaga
     7921 tctcgggtgt ggaggatcgc ttcaacgcct cgctgggtac gtaccacgat ctgctgaaga
     7981 tcatcaagga taaggatttc ctggataacg aggagaacga ggatatcctg gaggatatcg
     8041 tgctgacgct gacgctgttc gaggatcgcg agatgatcga ggagcgcctg aagacgtacg
     8101 cccacctgtt cgatgataag gtgatgaagc agctgaagcg ccgccgctac acgggttggg
     8161 gtcgcctgtc gcgcaagctg atcaacggta tccgcgataa gcagtcgggt aagacgatcc
     8221 tggatttcct gaagtcggat ggtttcgcca accgcaactt catgcagctg atccacgatg
     8281 attcgctgac gttcaaggag gatatccaga aggcccaggt gtcgggtcag ggtgattcgc
     8341 tgcacgagca catcgccaac ctggccggtt cgccggccat caagaagggt atcctgcaga
     8401 cggtgaaggt ggtggatgag ctggtgaagg tgatgggtcg ccacaagccg gagaacatcg
     8461 tgatcgagat ggcccgcgag aaccagacga cgcagaaggg tcagaagaac tcgcgcgagc
     8521 gcatgaagcg catcgaggag ggtatcaagg agctgggttc gcagatcctg aaggagcacc
     8581 cggtggagaa cacgcagctg cagaacgaga agctgtacct gtactacctg cagaacggtc
     8641 gcgatatgta cgtggatcag gagctggata tcaaccgcct gtcggattac gatgtggatc
     8701 acatcgtgcc gcagtcgttc ctgaaggatg attcgatcga taacaaggtg ctgacgcgct
     8761 cggataagaa ccgcggtaag tcggataacg tgccgtcgga ggaggtggtg aagaagatga
     8821 agaactactg gcgccagctg ctgaacgcca agctgatcac gcagcgcaag ttcgataacc
     8881 tgacgaaggc cgagcgcggt ggtctgtcgg agctggataa ggccggtttc atcaagcgcc
     8941 agctggtgga gacgcgccag atcacgaagc acgtggccca gatcctggat tcgcgcatga
     9001 acacgaagta cgatgagaac gataagctga tccgcgaggt gaaggtgatc acgctgaagt
     9061 cgaagctggt gtcggatttc cgcaaggatt tccagttcta caaggtgcgc gagatcaaca
     9121 actaccacca cgcccacgat gcctacctga acgccgtggt gggtacggcc ctgatcaaga
     9181 agtacccgaa gctggagtcg gagttcgtgt acggtgatta caaggtgtac gatgtgcgca
     9241 agatgatcgc caagtcggag caggagatcg gtaaggccac ggccaagtac ttcttctact
     9301 cgaacatcat gaacttcttc aagacggaga tcacgctggc caacggtgag atccgcaagc
     9361 gcccgctgat cgagacgaac ggtgagacgg gtgagatcgt gtgggataag ggtcgcgatt
     9421 tcgccacggt gcgcaaggtg ctgtcgatgc cgcaggtgaa catcgtgaag aagacggagg
     9481 tgcagacggg tggtttctcg aaggagtcga tcctgccgaa gcgcaactcg gataagctga
     9541 tcgcccgcaa gaaggattgg gatccgaaga agtacggtgg tttcgattcg ccgacggtgg
     9601 cctactcggt gctggtggtg gccaaggtgg agaagggtaa gtcgaagaag ctgaagtcgg
     9661 tgaaggagct gctgggtatc acgatcatgg agcgctcgtc gttcgagaag aacccgatcg
     9721 atttcctgga ggccaagggt tacaaggagg tgaagaagga tctgatcatc aagctgccga
     9781 agtactcgct gttcgagctg gagaacggtc gcaagcgcat gctggcctcg gccggtgagc
     9841 tgcagaaggg taacgagctg gccctgccgt cgaagtacgt gaacttcctg tacctggcct
     9901 cgcactacga gaagctgaag ggttcgccgg aggataacga gcagaagcag ctgttcgtgg
     9961 agcagcacaa gcactacctg gatgagatca tcgagcagat ctcggagttc tcgaagcgcg
    10021 tgatcctggc cgatgccaac ctggataagg tgctgtcggc ctacaacaag caccgcgata
    10081 agccgatccg cgagcaggcc gagaacatca tccacctgtt cacgctgacg aacctgggtg
    10141 ccccggccgc cttcaagtac ttcgatacga cgatcgatcg caagcgctac acgtcgacga
    10201 aggaggtgct ggatgccacg ctgatccacc agtcgatcac gggtctgtac gagacgcgca
    10261 tcgatctgtc gcagctgggt ggtgatggtt cgccgaagaa gaagcgcaag gtgtaagcgg
    10321 ggcttgcgac caggttgtag gctatggaat agtctatcat tgagttgtta tttctcttat
    10381 tatcagtaag tttccttttt agataaacta cgatcttgtg aagaacgtaa cagaatattt
    10441 ggttttgaaa agtacttgac ttagtttcaa acgcttaaat tgttgaaaac aatttaattt
    10501 catttatgac aataaaaaac ctgaaataat caaaacagta atgatgcatt ttgaatgaaa
    10561 atcgagaaaa agatatcgta tggttccgta aatgtcctcc cgggagaacc ttcccccggg
    10621 gcgctggtca ctccgggtgt tggccaatgt cctaccaatt tggtcccgtc taggtttctt
    10681 tgaattgctc aattgcttca attgctttag atcaaaaatt ccttcagtgg cttcaagaag
    10741 gcaacaacca gcacatgctg attcattttg gcgcagaagt tcgttaaatt gaatttaaaa
    10801 cagaatattt tatagttatg atcaattttc tccgaaaaag atcaacggct tcaccttaaa
    10861 tgcccaattg aattcaatag agtagcgatt ctcaaccttc ttctaggccg gtaccccctt
    10921 ccatgtaaat caagtaggcc cggtaccccc gttgagaatc cctgcaatag agcaatgatt
    10981 tgattgcagt gttgatcaaa taaaacattt attacctacc atttatgatc acaagaaaat
    11041 gttgcactat ggtacattgg gtgggctagc tgaagagcaa gggcgaattc tgcagatatc
    11101 catcacactg gcggccgctc gagcatgcat ctagagggcc caattcgccc tatagtgagt
    11161 cgtattacaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
    11221 cccaacttaa tcgccttgca gc
//
