LOCUS       sequence-333383-       13640 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of Cas9 in Culex quinquefasciatus or other mosquitoes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13640)
  AUTHORS   Feng X, Lopez Del Amo V, Mameli E, Lee M, Bishop AL, Perrimon N, 
            Gantz VM
  TITLE     Optimized CRISPR tools and site-directed transgenesis towards gene 
            drive development in Culex quinquefasciatus mosquitoes.
  JOURNAL   Nat Commun. 2021 May 20;12(1):2960. doi: 10.1038/s41467-021-23239-0.
  PUBMED    34017003
REFERENCE   2  (bases 1 to 13640)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-05-20"; volume: "12(1):2960. doi"; pages: " 
            10.1038/s41467-021-23239-0"
COMMENT     Sequence Label: pVMG0212_Cq-nanos-Cas9
FEATURES             Location/Qualifiers
     source          1..13640
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(17..34)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(17..33)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(26..48)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      174..602
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(261..280)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     471..492
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             946..1740
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1000..1019)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1610..1629
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             join(1758..1826,1827..2618)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1976..1995)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2789..3377
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3278..3297
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3531..3548
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3665..3686
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3701..3706,3707..3724,3725..3731)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3739..3755
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3744..3766
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3763..3779
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3763..3779
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             8028..12131
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             8028..12131
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             12138..12158
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(join(13631..13640,1..10))
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(join(13632..13640,1..10))
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 agtcgtatta caattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
       61 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
      121 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggacgcgcc
      181 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
      241 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
      301 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
      361 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
      421 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
      481 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
      541 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
      601 ttttaacaaa attcagggcg caagggctgc taaaggaagc ggaacacgta gaaagccagt
      661 ccgcagaaac ggtgctgacc ccggatgaat gtcagctact gggctatctg gacaagggaa
      721 aacgcaagcg caaagagaaa gcaggtagct tgcagtgggc ttacatggcg atagctagac
      781 tgggcggttt tatggacagc aagcgaaccg gaattgccag ctggggcgcc ctctggtaag
      841 gttgggaagc cctgcaaagt aaactggatg gctttcttgc cgccaaggat ctgatggcgc
      901 aggggatcaa gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat
      961 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
     1021 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
     1081 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg
     1141 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
     1201 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatcc
     1261 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
     1321 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
     1381 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
     1441 gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc
     1501 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
     1561 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
     1621 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
     1681 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
     1741 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     1801 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     1861 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     1921 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     1981 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     2041 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     2101 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     2161 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     2221 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     2281 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     2341 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     2401 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     2461 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     2521 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     2581 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     2641 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     2701 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     2761 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     2821 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     2881 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     2941 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     3001 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     3061 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     3121 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     3181 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     3241 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     3301 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     3361 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     3421 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     3481 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     3541 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     3601 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     3661 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     3721 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     3781 atgattacgc caagcttggt accgagctcg gatccactag taacggccgc cagtgtgctg
     3841 gaattcgccc ttatgtcctt tttaatcacg attcacagaa ataaaaattt cacaccgttc
     3901 acgcaaggca ctgcgactaa tgttaaggca aagttagatt ttttaagggt ccacccgttt
     3961 tgtctggcaa ggcaacaaat gtgaagagag actgacggct gcacggaaag gccgaccgac
     4021 ttttatacgt ccgccgaacc cgaagtcggg ccggcgaagc cggagccagc cagaactggg
     4081 aggaacataa cgtaaaaaag tgcgtctctc tttctctcgc tccccgatgg ttgttttccg
     4141 acgaaggaac attgaagttt gggagagttg ggatttttga atcgaactaa tcaatcctca
     4201 aaataattca ataaaaaaat aatcctaatt ataattatta aaaaaaataa aagcgacaac
     4261 agagttgatt ttttgtttga aaaataatgt ttatgaaatt tagtttaaaa tgtgttttca
     4321 atatgtggat aaatttttta aagcttcttt gcaataagag gcatttttag taacaaaaca
     4381 acagaacaaa aaaacacttt ttcattttca cgccagcaat ttatctgaac tttttacgtc
     4441 tgtgactttt cgtttgacat ttgcccagtc agtattttag cgaattctaa ccgagctgga
     4501 cgttcagaat tccggctaaa atttcaaaat gattttaact gtattctgat aaaatcagca
     4561 tttggtagaa gaattcatga actttttttc taaagttata ctactttcag tatttttttg
     4621 tctttgtagt tatttatgtt aaaatttatt tttgatgaaa ataaacattg ttttcaattt
     4681 atttaaaatc aatcgattaa agttaatcaa gacactactc gttcacccta ccctgtaaca
     4741 ccaaccgaag ttatccgcgc ccgcgaatcg acgaaccaca acaaaacgat ccaaacaacg
     4801 ataaaggaac ctccagatcc cctcataacc cggcccaaac cccgtgtacg aatcagctgc
     4861 tgtggggcga agtttttccg acttgtgccg gcgacgacgg cgattttcgc cgcatttgct
     4921 ccacttttgg cagaataatg ctgccataat tccatttttg gaatttgtcg aggccaatta
     4981 attgccttat gcgagccaag gttctataaa ttggtggtgc agataattcc aagatgctct
     5041 actcgaagtg gctgtctatg tgtttagatg gtttcaagaa tattggtcaa attttaagag
     5101 gaagctaact ctatttgatt ttttttggaa agtcatttgg ggaatccaag aaacatgtat
     5161 ttttgaactt ataatttaaa aaaacccatc ctccaaggaa aaggttttat ttttcaatcg
     5221 ttgttagaac atgtttttag aacccccaaa atgactgtcc attgattggt tgaccaattg
     5281 agtctaccaa ttcttcctcc tccgggtttt tgattctgga aattaaaagg aaccctagaa
     5341 tttaaaaaaa aatgtttttc ctaattgttt cgcttgtgta gtccagttat aacacttatt
     5401 tattattttt tcatcaaatt tatcgttgaa ttttcattgt acagattgtt tgcattattt
     5461 ggaggaggac tagcaacttt tttttggagg ggtattttgg aggggtattt tggaggagta
     5521 ttttagaggg gtattttgga ggggtatctg taaggggtat ctgtaaggaa attttctgat
     5581 caatttggtg tcttcggcaa agttgtaggt attgctgagg actattgaga aaaaaatttg
     5641 gtcacggaaa ataaattgcc gattttttta ttgacttttt ttttcacaaa aactcaattt
     5701 ccgaaaataa cgtatttttt gattttcgag attttttgat atgttttaga gaacaaaaac
     5761 ccgcatcttt tgaaccatag agaagtatgg tcaaaaaaat ctgccaccga gttatagtga
     5821 tagtgatttt tgaaaaaaaa acagagattt tttaaaaaaa atctaaattt tatgcaaaaa
     5881 attcgaccta attttttcat tgtgaaattg aatttgcaat cgaaaagtac tttacacatt
     5941 ttttgataaa gggctccgtt tttaaaatat agccactgaa agtttgattt tagcgaaata
     6001 ttaacggttt ttcgatattt acaaaaaggg accatgagtg accatttcca aaaatatttt
     6061 ttttgaaaag ttcaaaaatt aaaaaaaaaa tataagagag acgttgaagg ttggacctat
     6121 ggttgcttta aagctagaag tcgcctgaca aaaaactttt tctagaaaca gataggaaac
     6181 ctgatgcaaa caaacctcca gctgccatgt aaacatattt tcaatgtgtt ggtaaatttc
     6241 tgactagaat atcttacacc ttaaccaaaa atcatgattt tctgagttca atatcccact
     6301 tttcatacga attgttcagt tcaacaaata ttaccatttt tatggttgta gtacctgaac
     6361 tcaaaaaatc gtatgaaaag tgggatattg aactcagaaa atcatgattt ttagtaaggg
     6421 agtacggcat tctagaccca gtaaaaaaaa tataattcaa cccataaatg acgaacttct
     6481 cgtcacagtg tgctgatgca aacaatgatc gagctcgagc tgtcacaacc ctgcgaagta
     6541 tgttggccaa catatcgggc tgacagtcaa aaaaccacct agaatttgca tgacacaaaa
     6601 cttttgctag aaaaagatag gaaacctgat gcaaacaaac gtccagctgc catgtaaaca
     6661 tactttgaat gtgtaagtaa atttctgact agaaagcctt atttaaagtc caataaacaa
     6721 cacttttcta tctccacgta cgcccggtgg ccgatgtcgt acatgagaaa caatttaaaa
     6781 aaaacaacgt gttatcttgt aagtaattaa acacggaatg gctttactga atccatttga
     6841 caaccggaac ctcctctacg taatgcgctg tcgtgacctg ggcgttccag acgccaagtt
     6901 cgagtccagt ggtgtgcgga ctggtgggga ccatcgctga taaggttgtc ccaacaaaca
     6961 ctttcatttt ttgcttttag ggtgttttag aatatctctg acacaaggcg atttaaaaaa
     7021 aacactccag ctctattttt ttttatttcg acggtgacat ttcaaaaggc atcatgtaca
     7081 ttttgacact ttctgggtta ttgcatattc taaaagtact cctaataagc tacctttcca
     7141 ccaaaaatga gcaaaagtta cttcagtaaa gtctgtttaa tcatgatttg aaataaagta
     7201 acataaacaa aatctctgcc atcagcagtc cctgtttata tagccctgtc aacctgtcaa
     7261 acaaaagact acataaacct cgtgacgaaa aaaaagctac atgaacaaag cgccatgtac
     7321 attcggcgaa gaaaaacgaa tcataacaaa gcgtccccct caatgacagc agggtgatat
     7381 agacgttggt gatttcaaaa ggagttatgt ttgtttttct taaatataac gatggaaata
     7441 atgttttatt gaatttggat gatcaagtat caatgaatgc aacgtttttc atcgtttgtt
     7501 gttattagaa catcttattt tgcttttata ttaaaatggg aattgaaaat agatgctcaa
     7561 cattcaaatg cgtttttctc aaaacgcatg gtttgtacat gatgcttttt gaaatgttgg
     7621 cgtcgatttt accgaaagtg actcatctgg acatttccag tctgtgactt ttaaggagtt
     7681 tcactcattt tcattcaaca gtgtacgaca aacgtcaaac gaatttgtgc gtcaccttca
     7741 gcaaatttca tcaccttgct cttggagcca cggcagagag gcagcttttt ccttttggtc
     7801 ctgttttatc ctgatttcgt ttattttctc gtatttttta cattgtgctt tttgtaaaat
     7861 ttgataactt taatccctat ttgtatgttg tgaaaacata aatctgctaa ttgtgtgcct
     7921 caagtgccgt gaatttcgcc caacggtaag aatcgtgtcc tttttttgac gatgacgcaa
     7981 aacctaacct caaagttccc ccttcccggc aaggtccttt ctaaaacatg gataagaagt
     8041 actcgatcgg tctggatatc ggtacgaact cggtgggttg ggccgtgatc acggatgagt
     8101 acaaggtgcc gtcgaagaag ttcaaggtgc tgggtaacac ggatcgccac tcgatcaaga
     8161 agaacctgat cggtgccctg ctgttcgatt cgggtgagac ggccgaggcc acgcgcctga
     8221 agcgcacggc ccgccgccgc tacacgcgcc gcaagaaccg catctgctac ctgcaggaga
     8281 tcttctcgaa cgagatggcc aaggtggatg attcgttctt ccaccgcctg gaggagtcgt
     8341 tcctggtgga ggaggataag aagcacgagc gccacccgat cttcggtaac atcgtggatg
     8401 aggtggccta ccacgagaag tacccgacga tctaccacct gcgcaagaag ctggtggatt
     8461 cgacggataa ggccgatctg cgcctgatct acctggccct ggcccacatg atcaagttcc
     8521 gcggtcactt cctgatcgag ggtgatctga acccggataa ctcggatgtg gataagctgt
     8581 tcatccagct ggtgcagacg tacaaccagc tgttcgagga gaacccgatc aacgcctcgg
     8641 gtgtggatgc caaggccatc ctgtcggccc gcctgtcgaa gtcgcgccgc ctggagaacc
     8701 tgatcgccca gctgccgggt gagaagaaga acggtctgtt cggtaacctg atcgccctgt
     8761 cgctgggtct gacgccgaac ttcaagtcga acttcgatct ggccgaggat gccaagctgc
     8821 agctgtcgaa ggatacgtac gatgatgatc tggataacct gctggcccag atcggtgatc
     8881 agtacgccga tctgttcctg gccgccaaga acctgtcgga tgccatcctg ctgtcggata
     8941 tcctgcgcgt gaacacggag atcacgaagg ccccgctgtc ggcctcgatg atcaagcgct
     9001 acgatgagca ccaccaggat ctgacgctgc tgaaggccct ggtgcgccag cagctgccgg
     9061 agaagtacaa ggagatcttc ttcgatcagt cgaagaacgg ttacgccggt tacatcgatg
     9121 gtggtgcctc gcaggaggag ttctacaagt tcatcaagcc gatcctggag aagatggatg
     9181 gtacggagga gctgctggtg aagctgaacc gcgaggatct gctgcgcaag cagcgcacgt
     9241 tcgataacgg ttcgatcccg caccagatcc acctgggtga gctgcacgcc atcctgcgcc
     9301 gccaggagga tttctacccg ttcctgaagg ataaccgcga gaagatcgag aagatcctga
     9361 cgttccgcat cccgtactac gtgggtccgc tggcccgcgg taactcgcgc ttcgcctgga
     9421 tgacgcgcaa gtcggaggag acgatcacgc cgtggaactt cgaggaggtg gtggataagg
     9481 gtgcctcggc ccagtcgttc atcgagcgca tgacgaactt cgataagaac ctgccgaacg
     9541 agaaggtgct gccgaagcac tcgctgctgt acgagtactt cacggtgtac aacgagctga
     9601 cgaaggtgaa gtacgtgacg gagggtatgc gcaagccggc cttcctgtcg ggtgagcaga
     9661 agaaggccat cgtggatctg ctgttcaaga cgaaccgcaa ggtgacggtg aagcagctga
     9721 aggaggatta cttcaagaag atcgagtgct tcgattcggt ggagatctcg ggtgtggagg
     9781 atcgcttcaa cgcctcgctg ggtacgtacc acgatctgct gaagatcatc aaggataagg
     9841 atttcctgga taacgaggag aacgaggata tcctggagga tatcgtgctg acgctgacgc
     9901 tgttcgagga tcgcgagatg atcgaggagc gcctgaagac gtacgcccac ctgttcgatg
     9961 ataaggtgat gaagcagctg aagcgccgcc gctacacggg ttggggtcgc ctgtcgcgca
    10021 agctgatcaa cggtatccgc gataagcagt cgggtaagac gatcctggat ttcctgaagt
    10081 cggatggttt cgccaaccgc aacttcatgc agctgatcca cgatgattcg ctgacgttca
    10141 aggaggatat ccagaaggcc caggtgtcgg gtcagggtga ttcgctgcac gagcacatcg
    10201 ccaacctggc cggttcgccg gccatcaaga agggtatcct gcagacggtg aaggtggtgg
    10261 atgagctggt gaaggtgatg ggtcgccaca agccggagaa catcgtgatc gagatggccc
    10321 gcgagaacca gacgacgcag aagggtcaga agaactcgcg cgagcgcatg aagcgcatcg
    10381 aggagggtat caaggagctg ggttcgcaga tcctgaagga gcacccggtg gagaacacgc
    10441 agctgcagaa cgagaagctg tacctgtact acctgcagaa cggtcgcgat atgtacgtgg
    10501 atcaggagct ggatatcaac cgcctgtcgg attacgatgt ggatcacatc gtgccgcagt
    10561 cgttcctgaa ggatgattcg atcgataaca aggtgctgac gcgctcggat aagaaccgcg
    10621 gtaagtcgga taacgtgccg tcggaggagg tggtgaagaa gatgaagaac tactggcgcc
    10681 agctgctgaa cgccaagctg atcacgcagc gcaagttcga taacctgacg aaggccgagc
    10741 gcggtggtct gtcggagctg gataaggccg gtttcatcaa gcgccagctg gtggagacgc
    10801 gccagatcac gaagcacgtg gcccagatcc tggattcgcg catgaacacg aagtacgatg
    10861 agaacgataa gctgatccgc gaggtgaagg tgatcacgct gaagtcgaag ctggtgtcgg
    10921 atttccgcaa ggatttccag ttctacaagg tgcgcgagat caacaactac caccacgccc
    10981 acgatgccta cctgaacgcc gtggtgggta cggccctgat caagaagtac ccgaagctgg
    11041 agtcggagtt cgtgtacggt gattacaagg tgtacgatgt gcgcaagatg atcgccaagt
    11101 cggagcagga gatcggtaag gccacggcca agtacttctt ctactcgaac atcatgaact
    11161 tcttcaagac ggagatcacg ctggccaacg gtgagatccg caagcgcccg ctgatcgaga
    11221 cgaacggtga gacgggtgag atcgtgtggg ataagggtcg cgatttcgcc acggtgcgca
    11281 aggtgctgtc gatgccgcag gtgaacatcg tgaagaagac ggaggtgcag acgggtggtt
    11341 tctcgaagga gtcgatcctg ccgaagcgca actcggataa gctgatcgcc cgcaagaagg
    11401 attgggatcc gaagaagtac ggtggtttcg attcgccgac ggtggcctac tcggtgctgg
    11461 tggtggccaa ggtggagaag ggtaagtcga agaagctgaa gtcggtgaag gagctgctgg
    11521 gtatcacgat catggagcgc tcgtcgttcg agaagaaccc gatcgatttc ctggaggcca
    11581 agggttacaa ggaggtgaag aaggatctga tcatcaagct gccgaagtac tcgctgttcg
    11641 agctggagaa cggtcgcaag cgcatgctgg cctcggccgg tgagctgcag aagggtaacg
    11701 agctggccct gccgtcgaag tacgtgaact tcctgtacct ggcctcgcac tacgagaagc
    11761 tgaagggttc gccggaggat aacgagcaga agcagctgtt cgtggagcag cacaagcact
    11821 acctggatga gatcatcgag cagatctcgg agttctcgaa gcgcgtgatc ctggccgatg
    11881 ccaacctgga taaggtgctg tcggcctaca acaagcaccg cgataagccg atccgcgagc
    11941 aggccgagaa catcatccac ctgttcacgc tgacgaacct gggtgccccg gccgccttca
    12001 agtacttcga tacgacgatc gatcgcaagc gctacacgtc gacgaaggag gtgctggatg
    12061 ccacgctgat ccaccagtcg atcacgggtc tgtacgagac gcgcatcgat ctgtcgcagc
    12121 tgggtggtga tggttcgccg aagaagaagc gcaaggtgta aaattgtagt aatttgaacc
    12181 atgtaagaga aaaaagaaac tgtcgaatcg gtttcgctgc ctggcgcgcg gaaatcggca
    12241 taaagctagg tggaaagaag gtttgttccg gaaaaagtgt cattttgttt acacgtcgaa
    12301 tgtgtattct tgttttttta acgcactttt tatactgttt tttttttgta ctcttatttg
    12361 ttactgtata atcgagactt gtataagaaa aagcgttatg ttctgactat gatgaattca
    12421 ataaaatgat gttcgaacga atttctcaga aaagttatta tctacgtgtc catatttttt
    12481 taaacattta aaaaaaggtc aacttttcag aattcaaata agctcgttga ttaactgttg
    12541 gagcaaacca tgaaatgtga caaagttagg gaataaaaat atttcgtctc tctgtaagca
    12601 aaggaaaagt aaattgtgaa aagtaaaaat atgttctacc acaaatttaa attttcattg
    12661 gaaaaaactt gtacacgaaa agttcacgag agatgggcaa acaaaaagag aaagtcgctc
    12721 aaagagccgg tttactaaaa gagctaacga accatggctc acaaaaaaaa ccgcgattat
    12781 ttttgaaact ccgatctttt gaaagaaccg ttccaagatg taatgatttt gaaaatgtgt
    12841 gtctattttt atgtttattt aggctggtat aatagtagtt tatgcaacaa gttgcaaaaa
    12901 gaggattttt tcagcacgag tcgtacattt atccaacgag gttcaccgag ttggataaat
    12961 acgaagagtg ctgaaaaaat caagttttgc aacgagttcc atacaacatt ttttgcaatt
    13021 ccgaaaaata cccaatgagt gaaatttaat gtcaaatttt catgtatttt gtcaataaat
    13081 cgttgaaatc aaaaaaatgt tgaaaagtgt tacttttcga aacaagtgct gaaaagtaga
    13141 acttttcagc atttattttg aaaagtgttg ctattcgatt ctgttatttt tggtacagaa
    13201 aagtatgcta tttcgtcgtt caagaatgac aggaaaagta agtagtttca cgacggaatc
    13261 gcaaaaattt tattttaaaa acatataaaa cgtgcaataa gctaaaattt atttaaaatg
    13321 cattccgctg cgtttaaaaa tatgtttagc atgtttgaat tgatttaaaa atcttttcaa
    13381 tttttgaaaa aaatcgatgt ttagtatcgc aaaaagtttt tttttgcatt ttttttgttt
    13441 tcgtcaaatc ttactttttt ttgaaaacta atgattgcat ttaaaaaaaa ttgcattcaa
    13501 atgttggctt gttatttcaa tttttaattt tttttcagac aaattttgag caaccttgct
    13561 caagggcgaa ttctgcagat atccatcaca ctggcggccg ctcgagcatg catctagagg
    13621 gcccaattcg ccctatagtg
//
