LOCUS       sequence-333386-       12349 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of Cas9 in Culex quinquefasciatus or other mosquitoes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12349)
  AUTHORS   Feng X, Lopez Del Amo V, Mameli E, Lee M, Bishop AL, Perrimon N, 
            Gantz VM
  TITLE     Optimized CRISPR tools and site-directed transgenesis towards gene 
            drive development in Culex quinquefasciatus mosquitoes.
  JOURNAL   Nat Commun. 2021 May 20;12(1):2960. doi: 10.1038/s41467-021-23239-0.
  PUBMED    34017003
REFERENCE   2  (bases 1 to 12349)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-05-20"; volume: "12(1):2960. doi"; pages: " 
            10.1038/s41467-021-23239-0"
COMMENT     Sequence Label: pVMG0193_Cq-Rpl40-Cas9
FEATURES             Location/Qualifiers
     source          1..12349
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1739..1758)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1740..1758)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1765..1782)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1765..1781)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1774..1796)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1922..2350
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2009..2028)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2219..2240
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             2694..3488
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2748..2767)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3358..3377
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             join(3506..3574,3575..4366)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3724..3743)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4537..5125
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5026..5045
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5279..5296
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5413..5434
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5449..5454,5455..5472,5473..5479)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5487..5503
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5492..5514
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5511..5527
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5511..5527
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             complement(6470..6490)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(6497..10600)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(6497..10600)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
ORIGIN
        1 tgaccaaaaa gacccaatcg acgaaaatga acaaatcgac taaaatgacc caatcgacaa
       61 aaattaccaa atggaccaag acgaacaaat cgaccaaaat gaccaaatcg accaaaatta
      121 ccaaatcgac caaaatggcc aaatcgacca aaatgaacaa atcgactaaa ctgaccaaaa
      181 cgattaaaat ggcctaaacg accaaatcga ctaaaatgac caaatcgttc aaaattatca
      241 aaattaccca gattaccaaa tcgatcaaaa ttaacaaatc gaccaaaatt accaaatcga
      301 ccaaaatgaa caaatcaacc aaaatgacaa aatcgaccaa aattaccaaa ccaagcaaag
      361 agaacaaatc gactaaaatc taaatcgacc aaaatgacca gaatgattta aaaaaaaaaa
      421 attaacataa agtgcactgt tttcaaatta tctaaggtta atttttctaa aaaatatatt
      481 ttttaattta aaatgtgaag aaaaattaca tctgcagttt tttttacaaa aattaagaaa
      541 atttcctgca attttccttt tttaactttt gataacggac agcttaatgt tgtgatacag
      601 ttgtatactt aaaatttaaa ttttgtgaaa gaatagttta tctcagtgtc atctaataag
      661 cccttcattg cagctcgcga tatctctgga actattggtt tcagattggt taaattttta
      721 atgttaaagc ggtatacgca cttcggaagg aaccggtcaa gaaaaaaatc aaatgggtag
      781 cagcggcagc gcaagcaagt cagagagggt gaagaaaagc ccaaagaaat cgctccctct
      841 cctttgttct gctgttcgta aagctgtttc ttgacccctt tccttccgat ctgcatacta
      901 gccttaaaat agtaaaactt ttgtgatctg ctcttttcta tatatttttt tttaaatttt
      961 ataagcaatc accacttttt gaaaatgcta aaactatgca aatgctaaaa ctttgagatt
     1021 aatttgatta aaaaatggag gactataaga aattatggaa attattgatc aattttttaa
     1081 tgtcaccaga ttaagcctat aagctgaata gagctttatg acgtttcgcg tacacacact
     1141 agcgcaccat ttgattttgc tggccggaca aaatttaacc tcaatctttt tcgtgtacgt
     1201 acacgcaata catgtgcacg taaataactc tataagcaac aaccttctgc caagaaaacg
     1261 ttttaaaaat gtaaccttca attcagcgtg tgacacatag gtagcacaca cccgtcactg
     1321 agactccccc ttctctgata agcccacgtc acttcacgcc agcacgcgcg cacgagatcg
     1381 gtcaatcatc cgattttttc accccattca ttcattcatt ctcagctgtc ttcttttttc
     1441 aaccacctcg ctcttttgac cgccgattct tctggttgga gtttggaacc aggttccttt
     1501 tgcggtggcc aattagctgg tgccggagtg gtctagccgc tttaattgac attggtggcc
     1561 gcgttgagtt gcatttttca cctttgagga atgaggagta ggctgtgaaa ctgtggtgaa
     1621 gtttgtgtgt gagaagtaaa ccgtgagaaa agaagcaggc agaaaatgca agggcgaatt
     1681 ctgcagatat ccatcacact ggcggccgct cgagcatgca tctagagggc ccaattcgcc
     1741 ctatagtgag tcgtattaca attcactggc cgtcgtttta caacgtcgtg actgggaaaa
     1801 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     1861 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     1921 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     1981 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     2041 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     2101 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     2161 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     2221 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     2281 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     2341 aacgcgaatt ttaacaaaat tcagggcgca agggctgcta aaggaagcgg aacacgtaga
     2401 aagccagtcc gcagaaacgg tgctgacccc ggatgaatgt cagctactgg gctatctgga
     2461 caagggaaaa cgcaagcgca aagagaaagc aggtagcttg cagtgggctt acatggcgat
     2521 agctagactg ggcggtttta tggacagcaa gcgaaccgga attgccagct ggggcgccct
     2581 ctggtaaggt tgggaagccc tgcaaagtaa actggatggc tttcttgccg ccaaggatct
     2641 gatggcgcag gggatcaaga tctgatcaag agacaggatg aggatcgttt cgcatgattg
     2701 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg
     2761 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg
     2821 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg
     2881 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg
     2941 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc
     3001 tgtcatccca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc
     3061 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc
     3121 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc
     3181 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg
     3241 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct
     3301 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt
     3361 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc
     3421 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt
     3481 tcttctgaat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     3541 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     3601 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     3661 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     3721 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     3781 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     3841 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     3901 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     3961 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     4021 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     4081 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     4141 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     4201 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     4261 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     4321 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     4381 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     4441 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     4501 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     4561 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     4621 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     4681 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     4741 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     4801 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     4861 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     4921 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     4981 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     5041 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     5101 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     5161 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     5221 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     5281 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     5341 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     5401 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
     5461 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
     5521 ctatgaccat gattacgcca agcttggtac cgagctcgga tccactagta acggccgcca
     5581 gtgtgctgga attcgccctt gtcatcgtgg taagcgaatc tagaattttt cgaagtatct
     5641 tcaagagcag tgttggtatt atcgcgctct cgcgagaaaa atttctctct ctcgagttct
     5701 cgccgcgagt ctcgagctgc cgagagaaac tcaccgattg cccgtgctct cctccgctcg
     5761 cgaacgggct ttctcgcgag ccagaattgc ccgttcgcga gaagttgaac gaacaaaaaa
     5821 caacacaacg attgaagtta cacctttata ccatcgcctg gtccgtattc ataagcctga
     5881 taaacaaatt gctagcttgg gacgaacgga tagccagagg cgctcgcgtc gagagcgaga
     5941 acgcgaacga aaatgcgagc gcgagcgaga agagaaaagt actacaagtt ctctccctcg
     6001 ttcgcgagcg agaaatgctt tccttcttct cgctcgaatt ccaacactgt tcaagagtct
     6061 agagtcaaga gtctagattt ttatgtcgga tctgtacaga ttttttgttt tggatttatt
     6121 tcttttatta ttcggattta taaattaaac ttcctggatg tgtataattg caatacattc
     6181 tgaatctccc agtatgcctt aaaaatggta tcgtagttca acccaccagc ctgaaacagt
     6241 gatatttctt tcgactaact gagcacacaa gattcgtgtt gtatttttta aacttttttt
     6301 gtatgatttt atttattttc ttgctgtgac ttcaagcccc agccaaactc gcgctcggat
     6361 ctttgaagaa tgtttaattt tacaaacttg taacgcgacc cccggtcgga atcaacccag
     6421 aagcacaaca cacacacacc cgtccaaacg gcgaacgaat gggggcttac accttgcgct
     6481 tcttcttcgg cgaaccatca ccacccagct gcgacagatc gatgcgcgtc tcgtacagac
     6541 ccgtgatcga ctggtggatc agcgtggcat ccagcacctc cttcgtcgac gtgtagcgct
     6601 tgcgatcgat cgtcgtatcg aagtacttga aggcggccgg ggcacccagg ttcgtcagcg
     6661 tgaacaggtg gatgatgttc tcggcctgct cgcggatcgg cttatcgcgg tgcttgttgt
     6721 aggccgacag caccttatcc aggttggcat cggccaggat cacgcgcttc gagaactccg
     6781 agatctgctc gatgatctca tccaggtagt gcttgtgctg ctccacgaac agctgcttct
     6841 gctcgttatc ctccggcgaa cccttcagct tctcgtagtg cgaggccagg tacaggaagt
     6901 tcacgtactt cgacggcagg gccagctcgt tacccttctg cagctcaccg gccgaggcca
     6961 gcatgcgctt gcgaccgttc tccagctcga acagcgagta cttcggcagc ttgatgatca
     7021 gatccttctt cacctccttg taacccttgg cctccaggaa atcgatcggg ttcttctcga
     7081 acgacgagcg ctccatgatc gtgataccca gcagctcctt caccgacttc agcttcttcg
     7141 acttaccctt ctccaccttg gccaccacca gcaccgagta ggccaccgtc ggcgaatcga
     7201 aaccaccgta cttcttcgga tcccaatcct tcttgcgggc gatcagctta tccgagttgc
     7261 gcttcggcag gatcgactcc ttcgagaaac cacccgtctg cacctccgtc ttcttcacga
     7321 tgttcacctg cggcatcgac agcaccttgc gcaccgtggc gaaatcgcga cccttatccc
     7381 acacgatctc acccgtctca ccgttcgtct cgatcagcgg gcgcttgcgg atctcaccgt
     7441 tggccagcgt gatctccgtc ttgaagaagt tcatgatgtt cgagtagaag aagtacttgg
     7501 ccgtggcctt accgatctcc tgctccgact tggcgatcat cttgcgcaca tcgtacacct
     7561 tgtaatcacc gtacacgaac tccgactcca gcttcgggta cttcttgatc agggccgtac
     7621 ccaccacggc gttcaggtag gcatcgtggg cgtggtggta gttgttgatc tcgcgcacct
     7681 tgtagaactg gaaatccttg cggaaatccg acaccagctt cgacttcagc gtgatcacct
     7741 tcacctcgcg gatcagctta tcgttctcat cgtacttcgt gttcatgcgc gaatccagga
     7801 tctgggccac gtgcttcgtg atctggcgcg tctccaccag ctggcgcttg atgaaaccgg
     7861 ccttatccag ctccgacaga ccaccgcgct cggccttcgt caggttatcg aacttgcgct
     7921 gcgtgatcag cttggcgttc agcagctggc gccagtagtt cttcatcttc ttcaccacct
     7981 cctccgacgg cacgttatcc gacttaccgc ggttcttatc cgagcgcgtc agcaccttgt
     8041 tatcgatcga atcatccttc aggaacgact gcggcacgat gtgatccaca tcgtaatccg
     8101 acaggcggtt gatatccagc tcctgatcca cgtacatatc gcgaccgttc tgcaggtagt
     8161 acaggtacag cttctcgttc tgcagctgcg tgttctccac cgggtgctcc ttcaggatct
     8221 gcgaacccag ctccttgata ccctcctcga tgcgcttcat gcgctcgcgc gagttcttct
     8281 gacccttctg cgtcgtctgg ttctcgcggg ccatctcgat cacgatgttc tccggcttgt
     8341 ggcgacccat caccttcacc agctcatcca ccaccttcac cgtctgcagg atacccttct
     8401 tgatggccgg cgaaccggcc aggttggcga tgtgctcgtg cagcgaatca ccctgacccg
     8461 acacctgggc cttctggata tcctccttga acgtcagcga atcatcgtgg atcagctgca
     8521 tgaagttgcg gttggcgaaa ccatccgact tcaggaaatc caggatcgtc ttacccgact
     8581 gcttatcgcg gataccgttg atcagcttgc gcgacaggcg accccaaccc gtgtagcggc
     8641 ggcgcttcag ctgcttcatc accttatcat cgaacaggtg ggcgtacgtc ttcaggcgct
     8701 cctcgatcat ctcgcgatcc tcgaacagcg tcagcgtcag cacgatatcc tccaggatat
     8761 cctcgttctc ctcgttatcc aggaaatcct tatccttgat gatcttcagc agatcgtggt
     8821 acgtacccag cgaggcgttg aagcgatcct ccacacccga gatctccacc gaatcgaagc
     8881 actcgatctt cttgaagtaa tcctccttca gctgcttcac cgtcaccttg cggttcgtct
     8941 tgaacagcag atccacgatg gccttcttct gctcacccga caggaaggcc ggcttgcgca
     9001 taccctccgt cacgtacttc accttcgtca gctcgttgta caccgtgaag tactcgtaca
     9061 gcagcgagtg cttcggcagc accttctcgt tcggcaggtt cttatcgaag ttcgtcatgc
     9121 gctcgatgaa cgactgggcc gaggcaccct tatccaccac ctcctcgaag ttccacggcg
     9181 tgatcgtctc ctccgacttg cgcgtcatcc aggcgaagcg cgagttaccg cgggccagcg
     9241 gacccacgta gtacgggatg cggaacgtca ggatcttctc gatcttctcg cggttatcct
     9301 tcaggaacgg gtagaaatcc tcctggcggc gcaggatggc gtgcagctca cccaggtgga
     9361 tctggtgcgg gatcgaaccg ttatcgaacg tgcgctgctt gcgcagcaga tcctcgcggt
     9421 tcagcttcac cagcagctcc tccgtaccat ccatcttctc caggatcggc ttgatgaact
     9481 tgtagaactc ctcctgcgag gcaccaccat cgatgtaacc ggcgtaaccg ttcttcgact
     9541 gatcgaagaa gatctccttg tacttctccg gcagctgctg gcgcaccagg gccttcagca
     9601 gcgtcagatc ctggtggtgc tcatcgtagc gcttgatcat cgaggccgac agcggggcct
     9661 tcgtgatctc cgtgttcacg cgcaggatat ccgacagcag gatggcatcc gacaggttct
     9721 tggcggccag gaacagatcg gcgtactgat caccgatctg ggccagcagg ttatccagat
     9781 catcatcgta cgtatccttc gacagctgca gcttggcatc ctcggccaga tcgaagttcg
     9841 acttgaagtt cggcgtcaga cccagcgaca gggcgatcag gttaccgaac agaccgttct
     9901 tcttctcacc cggcagctgg gcgatcaggt tctccaggcg gcgcgacttc gacaggcggg
     9961 ccgacaggat ggccttggca tccacacccg aggcgttgat cgggttctcc tcgaacagct
    10021 ggttgtacgt ctgcaccagc tggatgaaca gcttatccac atccgagtta tccgggttca
    10081 gatcaccctc gatcaggaag tgaccgcgga acttgatcat gtgggccagg gccaggtaga
    10141 tcaggcgcag atcggcctta tccgtcgaat ccaccagctt cttgcgcagg tggtagatcg
    10201 tcgggtactt ctcgtggtag gccacctcat ccacgatgtt accgaagatc gggtggcgct
    10261 cgtgcttctt atcctcctcc accaggaacg actcctccag gcggtggaag aacgaatcat
    10321 ccaccttggc catctcgttc gagaagatct cctgcaggta gcagatgcgg ttcttgcggc
    10381 gcgtgtagcg gcggcgggcc gtgcgcttca ggcgcgtggc ctcggccgtc tcacccgaat
    10441 cgaacagcag ggcaccgatc aggttcttct tgatcgagtg gcgatccgtg ttacccagca
    10501 ccttgaactt cttcgacggc accttgtact catccgtgat cacggcccaa cccaccgagt
    10561 tcgtaccgat atccagaccg atcgagtact tcttatccat ggtgaatctg cgggaacgga
    10621 aaggcggata ttcaatgaaa acattgctcc atttgaggtt atgttcaggt ggtgaaaaca
    10681 agatccagtt ttctagttta aaatcgaatt tataatgaat cttttacgca aaacgaataa
    10741 accaaccaat tagctacaat ttctacgcta tttccaaaga attaaactat ttttaatcag
    10801 gaaaaatggc aaaaattgtg cactcactct ttcccaacgt gtccgcgttg ttagaagatt
    10861 tcccgagaaa gaaagcaaac tgtcagtggg gctgcggcct gtcagtggcg aacgtgcaag
    10921 cacccgtcac tgaaaactct gcaagggcgc ggagctttta gcacagggtg acaattttag
    10981 cgagtgagca aaagcgcggt gttacccaaa tttgggtaac aagcgttatt tcaaatgttc
    11041 aacagcagcc aacagcaatc tctagggttt gaattatgac cgagtcctta acctacgaat
    11101 tatataagaa tttccaatga tttaaaattg atcctcaaat gggatttatt gaccgaatcg
    11161 accaaaacga ccaaatcgac aataataata aaataattaa aattaccaaa ataactaaat
    11221 caaccaaatt aaccaaaatg tgcaaaacga ctttattgac ctaaacgacc gaaatggcct
    11281 aaacgaccgg aatgaccaaa attatcaaaa ttaccgaaac gaccaaaatt atcaaaatga
    11341 tcaaatcaac caaaattact aaatcgacct taacaaccaa attggcctaa actaccaaat
    11401 cgaccttgaa atcgactgaa ataaccaaat cgacaaaaat tacctaatcg aaattgccaa
    11461 aacgttccaa attatcaaaa ttaccaaatc gaccaaattg acctaatcga caaaaaagac
    11521 ctaatcaaca aaaatgacca aatcaaccaa aattaccaaa tcgaccaaaa tggccaaaac
    11581 gcccaaaatt accaatacgt tcaaaattat caaaattacc aatattatca aatcgaccaa
    11641 aatgacctaa ccaaacagaa tgaccatatc gaccaaaatg aacaaatcga ataaaatgac
    11701 caaaacgatg aatatggcct aaacgaccaa atcgactaaa attaccaaaa ttaccaaaat
    11761 gaacaaatcg actaaaataa cccaattgac aaaaatgacc aaaacgatca aaatggccaa
    11821 atcgacccaa tcgaccaaaa tgagcaaatc gaccaaaatg agcatatcga ccaaaatggc
    11881 caaaacgacc aaaattatca aaattaccaa atcgaccaaa atgaaccaat cgacaaaaat
    11941 gaccaaaacg attaaaatgg cctaaacgac caaatcgacc aaaattacca aaacgatcaa
    12001 aattatcaaa ataaccaaat cgaccaaatg atctgtcgat cgacaattaa aattgcctta
    12061 acgaccaaaa cgactagaat taccaaaatg accaaaatga acaaattgac taaaatggca
    12121 caatcgacaa aaatggccaa atcgaccaag ctgaacaaat tgaccaaaat taccaaatcg
    12181 accacaatgg tctaaacgac caaaatgacc caatcgacca aaatgactca atcgaccaaa
    12241 atgaccaaaa cgatcaaata gaccaaatcg accaaaatgg ccaaatcgac caaaatgcgc
    12301 aaatcgacca aaattaccaa aacgttcaaa attatcaata ttaccaaat
//
