LOCUS       sequence-333390-       12239 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12239)
  AUTHORS   Verma P, Zhou Y, Cao Z, Deraska PV, Deb M, Arai E, Li W, Shao Y, 
            Puentes L, Li Y, Patankar S, Mach RH, Faryabi RB, Shi J, Greenberg 
            RA
  TITLE     ALC1 links chromatin accessibility to PARP inhibitor response in 
            homologous recombination-deficient cells.
  JOURNAL   Nat Cell Biol. 2021 Jan 18. pii: 10.1038/s41556-020-00624-3. doi: 
            10.1038/s41556-020-00624-3.
  PUBMED    33462394
REFERENCE   2  (bases 1 to 12239)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2021 Jan 18. pii: 10.1038/s41556-020-00624-3. doi: 
            10.1038/s41556-020-00624-3."
COMMENT     Sequence Label: SaLgCG
FEATURES             Location/Qualifiers
     source          1..12239
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..181
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    228..353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    846..1079
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_RNA        complement(1141..1216)
                     /label=Sa gRNA scaffold
                     /note="guide RNA scaffold for the Staphylococcus aureus 
                     CRISPR/Cas9 system"
     promoter        complement(3098..3346)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(3156..3175)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(3326..3346)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     misc_feature    3353..3470
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3539..3750
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      3770..3779
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3779..3802
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3809..3829
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3854..7009
                     /codon_start=1
                     /product="Cas9 endonuclease from the Staphylococcus aureus 
                     Type II CRISPR/Cas system"
                     /label=SaCas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="KRNYILGLDIGITSVGYGIIDYETRDVIDAGVRLFKEANVENNEG
                     RRSKRGARRLKRRRRHRIQRVKKLLFDYNLLTDHSELSGINPYEARVKGLSQKLSEEEF
                     SAALLHLAKRRGVHNVNEVEEDTGNELSTKEQISRNSKALEEKYVAELQLERLKKDGEV
                     RGSINRFKTSDYVKEAKQLLKVQKAYHQLDQSFIDTYIDLLETRRTYYEGPGEGSPFGW
                     KDIKEWYEMLMGHCTYFPEELRSVKYAYNADLYNALNDLNNLVITRDENEKLEYYEKFQ
                     IIENVFKQKKKPTLKQIAKEILVNEEDIKGYRVTSTGKPEFTNLKVYHDIKDITARKEI
                     IENAELLDQIAKILTIYQSSEDIQEELTNLNSELTQEEIEQISNLKGYTGTHNLSLKAI
                     NLILDELWHTNDNQIAIFNRLKLVPKKVDLSQQKEIPTTLVDDFILSPVVKRSFIQSIK
                     VINAIIKKYGLPNDIIIELAREKNSKDAQKMINEMQKRNRQTNERIEEIIRTTGKENAK
                     YLIEKIKLHDMQEGKCLYSLEAIPLEDLLNNPFNYEVDHIIPRSVSFDNSFNNKVLVKQ
                     EENSKKGNRTPFQYLSSSDSKISYETFKKHILNLAKGKGRISKTKKEYLLEERDINRFS
                     VQKDFINRNLVDTRYATRGLMNLLRSYFRVNNLDVKVKSINGGFTSFLRRKWKFKKERN
                     KGYKHHAEDALIIANADFIFKEWKKLDKAKKVMENQMFEEKQAESMPEIETEQEYKEIF
                     ITPHQIKHIKDFKDYKYSHRVDKKPNRELINDTLYSTRKDDKGNTLIVNNLNGLYDKDN
                     DKLKKLINKSPEKLLMYHHDPQTYQKLKLIMEQYGDEKNPLYKYYEETGNYLTKYSKKD
                     NGPVIKKIKYYGNKLNAHLDITDDYPNSRNKVVKLSLKPYRFDVYLDNGVYKFVTVKNL
                     DVIKKENYYEVNSKCYEEAKKLKKISNQAEFIASFYNNDLIKINGELYRVIGVNNDLLN
                     RIEVNMIDITYREYLENMNDKRPPRIIKTIASKTQSIKKYSTDILGNLYEVKSKKHPQI
                     IKKG"
     CDS             7010..7057
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             7073..7129
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(7130..7132,7133..7135,7136..7849)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7130..7132,7133..7135,7136..7846)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7175..7196)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7436..7455)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7783..7804
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    7865..8453
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7918..7938)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8336..8347)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8525..8758
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8836..8957
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8873..8892)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8927..8946
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8997..9132
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9152..9171)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9153..9171)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9181..9198)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9181..9197)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9190..9212)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9339..9794
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9426..9445)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9636..9657
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9820..9924
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9925..9993,9994..10785)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MCIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10143..10162)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10956..11544
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11445..11464
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11698..11715
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11832..11853
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11868..11873,11874..11891,11892..11898)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11906..11922
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11911..11933
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11930..11946
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11930..11946
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11965..11985
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11967..11985
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        12013..12239
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
      661 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
      721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
      781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
      841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
      901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
      961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1081 gggatttggg gttgctctgg aaaactcgcc atttgtctca agatctagaa ttcaaaaaaa
     1141 tctcgccaac aagttgacga gataaacacg gcattttgcc ttgtttaagt agattctgtt
     1201 tccagagtac ttaaacagag acgtacaaaa aagagcaaga agctaaaaaa gatttaaaaa
     1261 ttatttttag cgcagttaat ggaacaggaa ctaaatttac cccaaaaata ttacgtgaat
     1321 caggatataa cgttattgag gttgaagagc atgcatttga agatgaaaca tttaaaaatg
     1381 ttgtaaatcc aaatccagaa tttgatcctg catgaaaaat accgcttgaa tatggtatta
     1441 aacatgatgc agatattatt attatgaatg acccagatgc tgacagattt ggaatggcaa
     1501 taaaacatga tggtcatttt gtaagattag atggaaatca aacaggacca attttaattg
     1561 attgaaaatt atcaaatcta aaacgcttaa atagcattcc aaaaaatccg gctctatatt
     1621 caagttttgt aacaagtgat ttgggtgata gaatcgctca tgaaaaatat ggagttaata
     1681 ttgtaaaaac tttaactgga tttaaatgaa tgggtagaga aattgctaaa gaagaagata
     1741 acggattaaa ttttgttttt gcttatgaag aaagttatgg atatgtaatt gatgactcag
     1801 ctagagataa agatggaata caagcttcta tattaatagc agaggctgct tgattttata
     1861 aaaaacaaaa taaaacatta gtagactatt tagaagattt atttaaagaa atgggtgcat
     1921 attacacttt cactttaaac ttgaatttta aaccagaaga aaagaaatta aaaattgaac
     1981 cattaatgaa atcattgaga gcaacaccct taactcaaat tgctggactt aaagttgtta
     2041 atgttgaaga ctacatcgat ggaatgtata atatgccagg acaagactta ctaaaatttt
     2101 atttagaaga taagtcatga tttgctgttc gcccaagtgg aactgaacct aaactaaaaa
     2161 tttattttat aggtgttggt gaatctgttc aaaacgctaa agttaaagta gacgaaatta
     2221 ttaaagaatt aaaattaaaa atgaatatat aggagaaaaa atgaaactaa acaaatatat
     2281 agatcacaca ttattaaaac aagatgctac gaaagctgaa attaaacaat tatgtgatga
     2341 agcaattgaa tttgattttg caacagtttg tgttaattca tattgaacaa gctattgtaa
     2401 agaattatta aaaggcacaa atgtaggaat aacaaatgtt gtaggttttc ctctaggtgc
     2461 atgcacaaca gctacaaaag cattcgaagt ttctgaagca attaaagatg gtgcaacaga
     2521 aattgatatg gtattaaata ttggtgcatt aaaagacaaa aattatgaat tagttttaga
     2581 agacatgaaa gctgtaaaaa aagcagctgg atcacatgtt gttaaatgta ttatggaaaa
     2641 ttgtttatta acaaaagaag aaatcatgaa agcttgtgaa atagctgttg aagctggatt
     2701 agaatttgtt aaaacatcaa caggattttc aaaatcaggt gcaacatttg aagatgttaa
     2761 actaatgaag tcagttgtta aagacaatgc tttagttaaa gcagctggtg gagttagaac
     2821 atttgaagat gctcaaaaaa tgattgaagc aggagctgac cgcttaggaa caagtggtgg
     2881 agtagctatt attaaaggtg aagaaaacaa cgcgagttac taaaactagc gtttttttat
     2941 tttgctcatt tttattaaaa gtttgcaaaa aggaacataa aaattctaat tattgatact
     3001 aaagttatta aaaagaagat tttggttgat tttataaagg tcatagaata taatatttta
     3061 gcatgtgtat tttgtgtgct catttacaac cgtctccggt gtttcgtcct ttccacaaga
     3121 tatataaagc caagaaatcg aaatactttc aagttacggt aagcatatga tagtccattt
     3181 taaaacataa ttttaaaact gcaaactacc caagaaatta ttactttcta cgtcacgtat
     3241 tttgtactaa tatctttgtg tttacagtca aattaattct aattatctct ctaacagcct
     3301 tgtatcgtat atgcaaatat gaaggaatca tgggaaatag gccctcccac aattttaaaa
     3361 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
     3421 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
     3481 acagggacag cagagatcca ctttggcgcc ggctcgagtg gctccggtgc ccgtcagtgg
     3541 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3601 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3661 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3721 tttcgcaacg ggtttgccgc cagaacacag gtgtcgtgac gcgggatccg ccaccatgga
     3781 ttacaaagac gatgacgata agatggcccc aaagaagaag cggaaggtcg gtatccacgg
     3841 agtcccagca gccaagcgga actacatcct gggcctggac atcggcatca ccagcgtggg
     3901 ctacggcatc atcgactacg agacacggga cgtgatcgat gccggcgtgc ggctgttcaa
     3961 agaggccaac gtggaaaaca acgagggcag gcggagcaag agaggcgcca gaaggctgaa
     4021 gcggcggagg cggcatagaa tccagagagt gaagaagctg ctgttcgact acaacctgct
     4081 gaccgaccac agcgagctga gcggcatcaa cccctacgag gccagagtga agggcctgag
     4141 ccagaagctg agcgaggaag agttctctgc cgccctgctg cacctggcca agagaagagg
     4201 cgtgcacaac gtgaacgagg tggaagagga caccggcaac gagctgtcca ccaaagagca
     4261 gatcagccgg aacagcaagg ccctggaaga gaaatacgtg gccgaactgc agctggaacg
     4321 gctgaagaaa gacggcgaag tgcggggcag catcaacaga ttcaagacca gcgactacgt
     4381 gaaagaagcc aaacagctgc tgaaggtgca gaaggcctac caccagctgg accagagctt
     4441 catcgacacc tacatcgacc tgctggaaac ccggcggacc tactatgagg gacctggcga
     4501 gggcagcccc ttcggctgga aggacatcaa agaatggtac gagatgctga tgggccactg
     4561 cacctacttc cccgaggaac tgcggagcgt gaagtacgcc tacaacgccg acctgtacaa
     4621 cgccctgaac gacctgaaca atctcgtgat caccagggac gagaacgaga agctggaata
     4681 ttacgagaag ttccagatca tcgagaacgt gttcaagcag aagaagaagc ccaccctgaa
     4741 gcagatcgcc aaagaaatcc tcgtgaacga agaggatatt aagggctaca gagtgaccag
     4801 caccggcaag cccgagttca ccaacctgaa ggtgtaccac gacatcaagg acattaccgc
     4861 ccggaaagag attattgaga acgccgagct gctggatcag attgccaaga tcctgaccat
     4921 ctaccagagc agcgaggaca tccaggaaga actgaccaat ctgaactccg agctgaccca
     4981 ggaagagatc gagcagatct ctaatctgaa gggctatacc ggcacccaca acctgagcct
     5041 gaaggccatc aacctgatcc tggacgagct gtggcacacc aacgacaacc agatcgctat
     5101 cttcaaccgg ctgaagctgg tgcccaagaa ggtggacctg tcccagcaga aagagatccc
     5161 caccaccctg gtggacgact tcatcctgag ccccgtcgtg aagagaagct tcatccagag
     5221 catcaaagtg atcaacgcca tcatcaagaa gtacggcctg cccaacgaca tcattatcga
     5281 gctggcccgc gagaagaact ccaaggacgc ccagaaaatg atcaacgaga tgcagaagcg
     5341 gaaccggcag accaacgagc ggatcgagga aatcatccgg accaccggca aagagaacgc
     5401 caagtacctg atcgagaaga tcaagctgca cgacatgcag gaaggcaagt gcctgtacag
     5461 cctggaagcc atccctctgg aagatctgct gaacaacccc ttcaactatg aggtggacca
     5521 catcatcccc agaagcgtgt ccttcgacaa cagcttcaac aacaaggtgc tcgtgaagca
     5581 ggaagaaaac agcaagaagg gcaaccggac cccattccag tacctgagca gcagcgacag
     5641 caagatcagc tacgaaacct tcaagaagca catcctgaat ctggccaagg gcaagggcag
     5701 aatcagcaag accaagaaag agtatctgct ggaagaacgg gacatcaaca ggttctccgt
     5761 gcagaaagac ttcatcaacc ggaacctggt ggataccaga tacgccacca gaggcctgat
     5821 gaacctgctg cggagctact tcagagtgaa caacctggac gtgaaagtga agtccatcaa
     5881 tggcggcttc accagctttc tgcggcggaa gtggaagttt aagaaagagc ggaacaaggg
     5941 gtacaagcac cacgccgagg acgccctgat cattgccaac gccgatttca tcttcaaaga
     6001 gtggaagaaa ctggacaagg ccaaaaaagt gatggaaaac cagatgttcg aggaaaagca
     6061 ggccgagagc atgcccgaga tcgaaaccga gcaggagtac aaagagatct tcatcacccc
     6121 ccaccagatc aagcacatta aggacttcaa ggactacaag tacagccacc gggtggacaa
     6181 gaagcctaat agagagctga ttaacgacac cctgtactcc acccggaagg acgacaaggg
     6241 caacaccctg atcgtgaaca atctgaacgg cctgtacgac aaggacaatg acaagctgaa
     6301 aaagctgatc aacaagagcc ccgaaaagct gctgatgtac caccacgacc cccagaccta
     6361 ccagaaactg aagctgatta tggaacagta cggcgacgag aagaatcccc tgtacaagta
     6421 ctacgaggaa accgggaact acctgaccaa gtactccaaa aaggacaacg gccccgtgat
     6481 caagaagatt aagtattacg gcaacaaact gaacgcccat ctggacatca ccgacgacta
     6541 ccccaacagc agaaacaagg tcgtgaagct gtccctgaag ccctacagat tcgacgtgta
     6601 cctggacaat ggcgtgtaca agttcgtgac cgtgaagaat ctggatgtga tcaaaaaaga
     6661 aaactactac gaagtgaata gcaagtgcta tgaggaagct aagaagctga agaagatcag
     6721 caaccaggcc gagtttatcg cctccttcta caacaacgat ctgatcaaga tcaacggcga
     6781 gctgtataga gtgatcggcg tgaacaacga cctgctgaac cggatcgaag tgaacatgat
     6841 cgacatcacc taccgcgagt acctggaaaa catgaacgac aagaggcccc ccaggatcat
     6901 taagacaatc gcctccaaga cccagagcat taagaagtac agcacagaca ttctgggcaa
     6961 cctgtatgaa gtgaaatcta agaagcaccc tcagatcatc aaaaagggca agcgtcctgc
     7021 tgctactaag aaagctggtc aagctaagaa aaagaaagct agcggcagcg gcgccaccaa
     7081 cttcagcctg ctgaagcagg ccggcgacgt ggaggagaac cccggcccca tggtgagcaa
     7141 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
     7201 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
     7261 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
     7321 cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt
     7381 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
     7441 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
     7501 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
     7561 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt
     7621 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
     7681 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac
     7741 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
     7801 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaga cgcgttaagt
     7861 cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     7921 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     7981 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     8041 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     8101 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     8161 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     8221 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct
     8281 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     8341 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     8401 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcgtcgact
     8461 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
     8521 ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg
     8581 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     8641 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     8701 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     8761 acgtatagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata
     8821 tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     8881 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     8941 ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc
     9001 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     9061 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     9121 tttttggagg cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc
     9181 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
     9241 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
     9301 cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt
     9361 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     9421 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     9481 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     9541 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     9601 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     9661 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     9721 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     9781 aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     9841 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     9901 caataatatt gaaaaaggaa gagtatgtgt attcaacatt tccgtgtcgc ccttattccc
     9961 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
    10021 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
    10081 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
    10141 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
    10201 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
    10261 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
    10321 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
    10381 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
    10441 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
    10501 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
    10561 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
    10621 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
    10681 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
    10741 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
    10801 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
    10861 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
    10921 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
    10981 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
    11041 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
    11101 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
    11161 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
    11221 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
    11281 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
    11341 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
    11401 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
    11461 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
    11521 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
    11581 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
    11641 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
    11701 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
    11761 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
    11821 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
    11881 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
    11941 tatgaccatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag
    12001 ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga
    12061 gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa
    12121 ggtggtacga tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa
    12181 ccactgaatt gccgcattgc agagatattg tatttaagtg cctagctcga tacataaac
//
