LOCUS       sequence-333416-        6497 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmTral_394-543.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6497)
  AUTHORS   Tritschler F, Eulalio A, Helms S, Schmidt S, Coles M, Weichenrieder 
            O, Izaurralde E, Truffault V
  TITLE     Similar modes of interaction enable Trailer Hitch and EDC3 to 
            associate with DCP1 and Me31B in distinct protein complexes.
  JOURNAL   Mol Cell Biol. 2008 Nov . 28(21):6695-708.
  PUBMED    18765641
REFERENCE   2  (bases 1 to 6497)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2008 Nov . 28(21):6695-708."
COMMENT     Sequence Label: pAc5.1B-EGFP-DmTral_394-543-V5His6_D
FEATURES             Location/Qualifiers
     source          1..6497
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     61..79
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        143..2652
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             join(2672..2674,2675..2677,2678..3388)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2672..2674,2675..2677,2678..3388)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2717..2738)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2978..2997)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3325..3346
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3890..3931
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             3941..3958
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3981..3998)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4031..4165
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4055..4074)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4109..4128
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(4766..5354)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4846..4865)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5525..6316,6317..6385))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6148..6167
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6386..6490)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
       61 aataggcgta tcacgaggcc ctttcgtctt caagaattaa ttctatattc taaaaacaca
      121 aatgatactt ctaaaaaaaa atcatgaatg gcatcaactc tgaatcaaat ctttgcagat
      181 gcacctactt ctcatttcca ctgtcacatc atttttccag atctcgctgc ctgttatgtg
      241 gcccacaaac caagacacgt tttatggcca ttaaagctgg ctgatcgtcg ccaaacacca
      301 aatacataat gaatatgtac acattcgaga aagaagcgat caaagaagcg tcttcgggcg
      361 gagtaggaga atgcggagga gaaggagaac gagctgatct agtatctctc cacaatccaa
      421 tgccaactga ccaactggcc atattcggag caatttgaag ccaatttcca tcgcctggcg
      481 atcgctccat tcttggctat atgtttttca ccgttacccg gggccatttt caaagactcg
      541 tcggcaagat aagattgtgt cactcgctgt ctctcttcat ttgtcgaaga atgctgagga
      601 atttcgcgat gacgtcggcg agtattttga agaatgagaa taatttgtat ttatacgaaa
      661 atcagttagt ggaattttct acaaaaacat gttatctata gataattttg ttgcaaaata
      721 tgttgactat gacaaagatt gtatgtatat acctttaatg tattctcatt ttcttatgta
      781 tttataatgg caatgatgat actgatgata ttttaagatg atgccagacc aaaaggcttg
      841 aatttctgcg tcttttgccg aacgcagtgc atgtgcaatt gttgtttttt ggaatattca
      901 attttcggac tgtccgcttt gatttcagtt tcttggctta ttcaaaaagc aaagtaaagc
      961 caaaaaagcg agatggcaat accaaatgcg gcaaaacggt agtggaagga aaggggtgcg
     1021 gggcagcgga aggaagggtg gggcggggcg tggcggggtc tgtggctggg cgcgacgtca
     1081 ccgacgttgg agccactcct ttgaccatgt gtgcgtgtgt gtattattcg tgtctcgcca
     1141 ctcgccggtt gtttttttct ttttatgctg cgctctctct agcgccatct cgcttacgca
     1201 tgctcaacgc accgcatgtt gccgtttcct tttatgcgtc attttggctc gaaataggca
     1261 attatttaaa caaagattag tcaacgaaaa cgctaaaata aataagtcta caatatggtt
     1321 acttattgcc atgtgtgtgc agccaacgat agcaacaaaa gcaacaacac aggtggcttt
     1381 ccctctttca ctttttgttt gcaagccgcg tgcgagcaag acggcacgac cggcaaacgc
     1441 aattacgctg acaaagagca gacgaagttt tggcgaaaaa catcaaggcg cctgatacga
     1501 atgcatttgc aataacaatt gcgatattta atattgttta tgaagctgtt tgacttcaaa
     1561 acacacaaaa aaaaaaataa aacaaattat ttgaaagaga attaggaatc ggacgcttat
     1621 cgttagggta acaacaagaa atgcttactg agtcacagcc tctggaaaac tgccgcaagc
     1681 cagagagaga gagaaaaaga gggagagcag cttagaccgc atgtgcttgt gtgtgaggcg
     1741 tctctctctt cgtctctgtt gcgcaaacgc atagactgca ctgaaaaaat cgattaccta
     1801 ttttttatga atgaatattt gcactattac tattcaaaac tattaagata gcaatcacat
     1861 tcaatagcca aatactatac cacctgagcg atgcaacgaa atgatcaatt tgagcaaaaa
     1921 tgctgcatat ttaggacggc atcattatag aaatgcttct tgctgtgtac ttttctctcg
     1981 tctggcagct gtttcgccgt tattgttaaa accggcttaa gttaggtgtg ttttctacga
     2041 ctagtgaatg ccctactaga agatgtgtgt tgcacaaaat gtccctggaa taaccaattt
     2101 gaagtgcaga tagcagtaaa cgtaagctaa tatgaatatt atttaactgt aatgttttaa
     2161 tatcgctgga cattactaat aaacccacta taaacacatg tacatatgta tgttttggca
     2221 tacaatgagt agttggggaa aaaatgtgta aaagcaccgt gaccatcaca gcataaagat
     2281 aaccagctga agtatcgaat atgagtaacc cccaaattga atcacatgcc gcaactgata
     2341 ggacccatgg aagtacactc ttcatggcga tatacaagac acacacaagc acgaacaccc
     2401 agttgcggag gaaattctcc gtaaatgaaa acccaatcgg cgaacaattc atacccatat
     2461 atggtaaaag ttttgaacgc gacttgagag cggagagcat tgcggctgat aaggttttag
     2521 cgctaagcgg gctttataaa acgggctgcg ggaccagttt tcatatcact accgtttgag
     2581 ttcttgtgct gtgtggatac tcctcccgac acaaagccgc tccatcagcc agcagtcgtc
     2641 taatccagag accccggatc ggggtaccat catggtgagc aagggcgagg agctgttcac
     2701 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
     2761 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
     2821 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca
     2881 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     2941 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
     3001 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     3061 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
     3121 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca
     3181 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     3241 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa
     3301 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     3361 cactctcggc atggacgagc tgtacaagcc aagcttgaat tctcagaaca agccgcggaa
     3421 caagatcaag ttcgagggcg acttcgattt cgagcaggca aacaacaagt tcgaggaact
     3481 gcgctcccaa ctggccaagc tcaaggtggc cgaggatggt gcacccaagc cagccaccaa
     3541 tgcaacggcc gccactgcaa ctgcaaccaa tgagcaggtg ggtgagaagg ttgaaggcgt
     3601 tcacacactg aatggcgaga ccgacaagaa ggatgattct ggcaacgaga ccggcgctgg
     3661 ggagcacgag cctgaggggg atgatgttgc tgtgtgctac gacaagacca aatcgttctt
     3721 cgacaacatc tcgtgcgagg ctgcccagga tcgcagcagg aacaagaaga acgattggcg
     3781 ccaggagcgc aagttgaaca cggagacctt cggagtgtcc tccacacgac gtggcagtta
     3841 ccgcggacgc aacggcggcc gctcgagtct agagggcccg cggttcgaag gtaagcctat
     3901 ccctaaccct ctcctcggtc tcgattctac gcgtaccggt catcatcacc atcaccattg
     3961 agtttaaacc cgctgatcag cctcgactgt gccttctaag gcctgagctc gctgatcagc
     4021 ctcgatcgag gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag
     4081 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
     4141 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt
     4201 tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtatggc
     4261 tgattatgat cagtcgaccg atgcccttga gagccttcaa cccagtcagc tccttccggt
     4321 gggcgcgggg catgactatc gtcgccgcac ttatgactgt cttctttatc atgcaactcg
     4381 taggacaggt gccggcagcg ctctgggtca ttttcggcga ggaccgcttt cgctggagcg
     4441 cgacgatgat cggcctgtcg cttgcggtat tcggaatctt gcacgccctc gctcaagcct
     4501 tcgtcactgg tcccgccacc aaacgtttcg gcgagaagca ggccattatc gccggcatgg
     4561 cggccgacgc gctgggctac gtcttgctgg cgttcgcgac gcgaggctgg atggccttcc
     4621 ccattatgat tcttctcgct tccggcggca tcgggatgcc cgcgttgcag gccatgctgt
     4681 ccaggcaggt agatgacgac catcagggac agcttcaagg ccagcaaaag gccaggaacc
     4741 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     4801 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     4861 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     4921 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     4981 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     5041 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     5101 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     5161 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     5221 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     5281 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     5341 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     5401 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     5461 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     5521 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     5581 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     5641 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     5701 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     5761 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     5821 gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     5881 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     5941 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     6001 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     6061 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     6121 gttgctcttg cccggcgtca acacgggata ataccgcgcc acatagcaga actttaaaag
     6181 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     6241 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     6301 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     6361 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     6421 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     6481 gggttccgcg cacattt
//
