LOCUS       sequence-33342-e         609 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Non-integrating (episomal) expression of human C-MYC, LIN28 and 
            NANOG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 609)
  AUTHORS   Okita K, Matsumura Y, Sato Y, Okada A, Morizane A, Okamoto S, Hong 
            H, Nakagawa M, Tanabe K, Tezuka KI, Shibata T, Kunisada T, Takahashi
            M, Takahashi J, Saji H, Yamanaka S
  TITLE     A more efficient method to generate integration-free human iPS 
            cells.
  JOURNAL   Nat Methods. 2011 May;8(5):409-12
  PUBMED    21460823
REFERENCE   2  (bases 1 to 609)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2011-05"; volume: "8(5)"; pages: "409-1"
COMMENT     Sequence Label: pCXLE-hMLN
FEATURES             Location/Qualifiers
     source          1..609
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(244..309)
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             complement(394..423)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 gtgcagctta ctctggtgca caaagacatc cactgggggg tcgagcgcga ccccggcgcg
       61 ggcggtcatg gacaggaagc cgaaccccat gcgcacgttg aaccacttac agatgcccgc
      121 accgtgcagc agctgaggct cgtccgccgc ccgggccgcg tcctccggcg cctcctcggg
      181 cgcctcttct gccgccttgg cgcagccacc tgcaaactgc tggttggaca cggagcccat
      241 gagtggacct ggattgcttt ctacatcccc agccagtttg agtaaatcaa agttaagagt
      301 ttgtttgaca ggagcgacaa ttttgagatc ccccgcacaa gagttccgta gctgttcaag
      361 tttgtgtttc aactgttctc gtcgtttccg caacaagtcc tcttcagaaa tgagcttttg
      421 ctcctctgct tggacggaca ggatgtatgc tgtggctttt ttaaggataa ctaccttggg
      481 ggccttttca ttgttttcca actccgggat ctggtcacgc agggcaaaaa agctccgttt
      541 tagctcgttc ctcctctggc gctccaagac gttgtgtgtt cgcctcttga cattctcctc
      601 ggtgtccga
//
