LOCUS       sequence-333426-        1295 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses AKTIP in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1295)
  AUTHORS   Feng Z, Yu CH
  TITLE     PI(3,4)P2-mediated membrane tubulation promotes integrin trafficking
            and invasive cell migration.
  JOURNAL   Proc Natl Acad Sci U S A. 2021 May 11;118(19). pii: 2017645118. doi:
            10.1073/pnas.2017645118.
  PUBMED    33947811
REFERENCE   2  (bases 1 to 1295)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2021-05-11"; volume: "118(19). pii: 
            2017645118. doi"; pages: " 10.1073/pnas.2017645118"
COMMENT     Sequence Label: mRuby2-AKTIP
FEATURES             Location/Qualifiers
     source          1..1295
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      64..73
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             70..780
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
ORIGIN
        1 cccaaagggg ggctaatagc agagctggtt tagtgaccgt cagatccgct agcgctaccg
       61 gtcgccacca tggtgtctaa gggcgaagag ctgatcaagg aaaatatgcg tatgaaggtg
      121 gtcatggaag gttcggtcaa cggccaccaa ttcaaatgca caggtgaagg agaaggcaat
      181 ccgtacatgg gaactcaaac catgaggatc aaagtcatcg agggaggacc cctgccattt
      241 gcctttgaca ttcttgccac gtcgttcatg tatggcagcc gtacttttat caagtacccg
      301 aaaggcattc ctgatttctt taaacagtcc tttcctgagg gttttacttg ggaaagagtt
      361 acgagatacg aagatggtgg agtcgtcacc gtcatgcagg acaccagcct tgaggatggc
      421 tgtctcgttt accacgtcca agtcagaggg gtaaactttc cctccaatgg tcccgtgatg
      481 cagaagaaga ccaagggttg ggagcctaat acagagatga tgtatccagc agatggtggt
      541 ctgaggggat acactcatat ggcactgaaa gttgatggtg gtggccatct gtcttgctct
      601 ttcgtaacaa cttacaggtc aaaaaagacc gtcgggaaca tcaagatgcc cggtatccat
      661 gccgttgatc accgcctgga aaggttagag gaaagtgaca atgaaatgtt cgtagtacaa
      721 cgcgaacacg cagttgccaa gttcgccggg cttggtggtg ggatggacga gctgtacaag
      781 tccggactca gatctcgagc tcaagcttcg aattctgcag tcgacatgaa ccctttctgg
      841 agcatgtcta caagctctgt acgcaaacga tctgaaggtg aagagaagac attaacaggg
      901 gacgtgaaaa ccagtcctcc acgaactgca ccaaagaaac agctgccttc tattcccaaa
      961 aatgctttgc ccataactaa gcctacatct cctgccccag cagcacagtc aacaaatggc
     1021 acgcatgcgt cctatggacc cttctacctg aatactctct ctgcagattt accttggtgt
     1081 gaagcaaagc taccagcgtc tatgtgcagc atctatcgct ctgcattaat gtattgagta
     1141 tatcatacgc atgactttac cagaatgggc gtattagttt acagttacat cctgataact
     1201 tcgatggtga ctgtcacggc tgggttcgaa ttctgttctt caccgctagt tgatccacct
     1261 cagggtagac cttggaagtg tggaaagaag accac
//
