LOCUS       sequence-333432-        1246 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Endophilin A1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Feng Z, Yu CH
  TITLE     PI(3,4)P2-mediated membrane tubulation promotes integrin trafficking
            and invasive cell migration.
  JOURNAL   Proc Natl Acad Sci U S A. 2021 May 11;118(19). pii: 2017645118. doi:
            10.1073/pnas.2017645118.
  PUBMED    33947811
REFERENCE   2  (bases 1 to 1246)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2021-05-11"; volume: "118(19). pii: 
            2017645118. doi"; pages: " 10.1073/pnas.2017645118"
COMMENT     Sequence Label: EndophilinA1-mRuby2
FEATURES             Location/Qualifiers
     source          1..1246
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(152..862)
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
     regulatory      859..868
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aagtcattaa agctgcataa acaagttaac aacaacaatt gcattcattt tatgtttcag
       61 gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtatg
      121 gctgattatg atctagagtc gcggccgctt acttgtacag ctcgtccatc ccaccaccaa
      181 gcccggcgaa cttggcaact gcgtgttcgc gttgtactac gaacatttca ttgtcacttt
      241 cctctaacct ttccaggcgg tgatcaacgg catggatacc gggcatcttg atgttcccga
      301 cggtcttttt tgacctgtaa gttgttacga aagagcaaga cagatggcca ccaccatcaa
      361 ctttcagtgc catatgagtg tatcccctca gaccaccatc tgctggatac atcatctctg
      421 tattaggctc ccaacccttg gtcttcttct gcatcacggg accattggag ggaaagttta
      481 cccctctgac ttggacgtgg taaacgagac agccatcctc aaggctggtg tcctgcatga
      541 cggtgacgac tccaccatct tcgtatctcg taactctttc ccaagtaaaa ccctcaggaa
      601 aggactgttt aaagaaatca ggaatgcctt tcgggtactt gataaaagta cggctgccat
      661 acatgaacga cgtggcaaga atgtcaaagg caaatggcag gggtcctccc tcgatgactt
      721 tgatcctcat ggtttgagtt cccatgtacg gattgccttc tccttcacct gtgcatttga
      781 attggtggcc gttgaccgaa ccttccatga ccaccttcat acgcatattt tccttgatca
      841 gctcttcgcc cttagacacc atggtggcga ccggtggatc ccgggcccgc ggtaccgtcg
      901 actgcagaat tcgaagctta tggggcaggc aaccagaatt tccacataat tgatggggaa
      961 gaagcctgaa tggccatgca gcatcccctc ataccagttc tcatcaattt gggttagtga
     1021 gtgtgatgat atcgccctct ttaaatccca ctccccttca ttttcagttc aaagtcgtac
     1081 aggagctcgc agcaggctga tcatttggac actggagttt gggatgctgt gtgggagaga
     1141 ccccaatggc ctgatactgt ctcagctgga ctcaagctca atcctgcaag atgaatacat
     1201 cagctgaatg agctgactat catctccagt ctgacgtgaa ctgacg
//
