LOCUS       sequence-333433-        1265 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses PIK3C2G in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Feng Z, Yu CH
  TITLE     PI(3,4)P2-mediated membrane tubulation promotes integrin trafficking
            and invasive cell migration.
  JOURNAL   Proc Natl Acad Sci U S A. 2021 May 11;118(19). pii: 2017645118. doi:
            10.1073/pnas.2017645118.
  PUBMED    33947811
REFERENCE   2  (bases 1 to 1265)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2021-05-11"; volume: "118(19). pii: 
            2017645118. doi"; pages: " 10.1073/pnas.2017645118"
COMMENT     Sequence Label: EGFP-PIK3C2G
FEATURES             Location/Qualifiers
     source          1..1265
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      65..74
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(71..73,74..76,77..787)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(71..73,74..76,77..787)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(116..137)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(377..396)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     724..745
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ccagttttgg agcttatagc agagctggtt tagtgaaccg tcagatccgc tagcgctacc
       61 ggtcgccacc atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
      121 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga
      181 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
      241 ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga
      301 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
      361 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
      421 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
      481 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa
      541 gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt
      601 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
      661 cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga
      721 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct
      781 gtacaagtcc ggactcagat ctcgagctca agcttcgaat tctgcagtcg acatggcata
      841 ttcttggcaa acggatccaa atcctaatga atcacacgaa aagcagtatg aacaccaaga
      901 atttctcttt gtaaatcaac cccattcttc tagccaagtc agtctgggtt ttgatcagat
      961 agtagatgag atcagtggca aattccacac tacgagagtg aaattgatga aacacctttt
     1021 tgtgcccact gcacaaatgg gactcaacag gcattcataa atgagcacac caatatcctg
     1081 atgattcact ctaaagcgtg actctcctgg catcagtagc aagcaccagc atgtttagtc
     1141 ttctggttac caaacctcaa tcgaataaga atgctctggg gagcatggaa acaccagggg
     1201 ctgaagatcg attcgattag cctctctctc cgttggtaaa ttattcctgg aagattgaat
     1261 gtgaa
//
