LOCUS       sequence-333439-        1304 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses INPP5J in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1304)
  AUTHORS   Feng Z, Yu CH
  TITLE     PI(3,4)P2-mediated membrane tubulation promotes integrin trafficking
            and invasive cell migration.
  JOURNAL   Proc Natl Acad Sci U S A. 2021 May 11;118(19). pii: 2017645118. doi:
            10.1073/pnas.2017645118.
  PUBMED    33947811
REFERENCE   2  (bases 1 to 1304)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2021-05-11"; volume: "118(19). pii: 
            2017645118. doi"; pages: " 10.1073/pnas.2017645118"
COMMENT     Sequence Label: mCherry-INPP5J
FEATURES             Location/Qualifiers
     source          1..1304
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        75..93
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     75..92
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      149..158
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             155..862
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGREFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(300..318)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     568..587
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     762..785
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     protein_bind    875..899
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ccccaggggg gaggctaata agcatgctcg tttagggaac cgccattctg cctggggacg
       61 tcggagcaag cttgatttag gtgacactat agaatacaag ctacttgttc tttttgcagg
      121 atcccatcga ttcgaattca aggctagcgc caccatggtg agcaagggcg aggaggacaa
      181 catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg
      241 ccgcgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc
      301 caagctgaag gtgaccaagg gcggccccct gcccttcgcc tgggacatcc tgtcccctca
      361 gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa
      421 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt
      481 ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct
      541 gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga
      601 ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag
      661 gctgaagctg aaggacggcg gccactacga cgccgaggtc aagaccacct acaaggccaa
      721 gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagctggaca tcacctccca
      781 caacgaggac tacaccatcg tggaacagta cgagcgcgcc gagggccgcc actccaccgg
      841 cggcatggac gagctgtaca agagatctcg aatcacaagt ttgtacaaaa aagttggcac
      901 catggccctc ccaaggcttg acacccagag tacagggcct ggcaggtgcc tgagccccaa
      961 ccttcaggcc caagaagccc cagccccagt caccacctcc tcttctacat ccaccctgtc
     1021 atcctcccct ggtcagctca gcctacctga agagcgaccc ggcttccgga tcactgtgtc
     1081 cacatggacg gtggcactgc atgccccaga cgatgtcaca tccctcttca cctgggcggt
     1141 ggtgacgacg cgacggccag actgatcgca tagatgcaga gtgaactcat gctcacagga
     1201 ctcaagacgc cctcttacga cagtgaatga acgttcatgg atgccctagc ccttcacctc
     1261 aggctggtga gattcgggag aagaatgaca gaggacattc tggt
//
