LOCUS       sequence-333441-        1283 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses INPP5K in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1283)
  AUTHORS   Feng Z, Yu CH
  TITLE     PI(3,4)P2-mediated membrane tubulation promotes integrin trafficking
            and invasive cell migration.
  JOURNAL   Proc Natl Acad Sci U S A. 2021 May 11;118(19). pii: 2017645118. doi:
            10.1073/pnas.2017645118.
  PUBMED    33947811
REFERENCE   2  (bases 1 to 1283)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2021-05-11"; volume: "118(19). pii: 
            2017645118. doi"; pages: " 10.1073/pnas.2017645118"
COMMENT     Sequence Label: mCherry-INPP5K
FEATURES             Location/Qualifiers
     source          1..1283
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        70..88
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     70..87
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      144..153
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             150..857
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGREFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(295..313)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     563..582
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     757..780
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     protein_bind    870..894
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 aaagtgggaa gctaatagca tgctcgttta gggaccgcca ttctgcctgg ggacgtcgga
       61 gcaagcttga tttaggtgac actatagaat acaagctact tgttcttttt gcaggatccc
      121 atcgattcga attcaaggct agcgccacca tggtgagcaa gggcgaggag gacaacatgg
      181 ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccgcg
      241 agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc
      301 tgaaggtgac caagggcggc cccctgccct tcgcctggga catcctgtcc cctcagttca
      361 tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt
      421 ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga
      481 ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg
      541 gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct
      601 cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga
      661 agctgaagga cggcggccac tacgacgccg aggtcaagac cacctacaag gccaagaagc
      721 ccgtgcagct gcccggcgcc tacaacgtca acatcaagct ggacatcacc tcccacaacg
      781 aggactacac catcgtggaa cagtacgagc gcgccgaggg ccgccactcc accggcggca
      841 tggacgagct gtacaagaga tctcgaatca caagtttgta caaaaaagtt ggcatgagct
      901 cgcggaagct gagcgggccg aaaggcagga ggctcagcat acacgtcgtg acttggaacg
      961 tgcttcggca gcgcctcctc tagatctcag tgacctgctt cagctgaaca accggaacct
     1021 caatcttgac atatatgtta ttggtttgca gatgaactct ggatcatagc ctcctttccg
     1081 atgctgcttt atgactcgtg agcagtttcc tcatgatgtg ctttcccctc tgagctcatc
     1141 aagtcttcca tgtcgtatgc aggcatctct actgatcttg acagtatcag cattgaccta
     1201 atatcgaatc tctactatcc actcgctcac acattgactg tctcgcatct tgggggaaat
     1261 cgaagtgtgg atcaaacatc gtc
//
