LOCUS       sequence-33346-e         432 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Integration-free (episomal) expression of EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 432)
  AUTHORS   Okita K, Matsumura Y, Sato Y, Okada A, Morizane A, Okamoto S, Hong 
            H, Nakagawa M, Tanabe K, Tezuka KI, Shibata T, Kunisada T, Takahashi
            M, Takahashi J, Saji H, Yamanaka S
  TITLE     A more efficient method to generate integration-free human iPS 
            cells.
  JOURNAL   Nat Methods. 2011 May;8(5):409-12
  PUBMED    21460823
REFERENCE   2  (bases 1 to 432)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2011-05"; volume: "8(5)"; pages: "409-1"
COMMENT     Sequence Label: pCXLE-EGFP
FEATURES             Location/Qualifiers
     source          1..432
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      12..21
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(49..68)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(130..152)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
ORIGIN
        1 gcccttgctc accatggtgg cgaattcttt gccaaaatga tgagacagca caataaccag
       61 cacgttgccc aggagctgta ggaaaaagaa gaaggcatga acatggttag cagaggctct
      121 agagccgccg gtcacacgcc agaagccgaa ccccgccctg ccccgtcccc cccgaaggca
      181 gccgtccccc cgcggacagc cccgaggctg gagagggaga aggggacggc ggcgcggcga
      241 cgcacgaagg ccctccccgc ccatttcctt cctgccggcg ccgcaccgct tcgccccgcg
      301 cccgctagag ggggtgcggc ggcgcctccc agatttcggc tccgcacaga tttgggacaa
      361 aggaagtccc tgcgccctct cgcacgatta ccataaaagg caatggctgc ggctcgccgc
      421 gcctcgacag cc
//
