LOCUS       sequence-333465-       12335 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses C-terminal flag-tagged CAD with mutation of reported 
            Erk1/2 and PKA phosphorylation site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12335)
  AUTHORS   Shin J, Mir H, Khurram MA, Fujihara KM, Dynlacht BD, Cardozo TJ, 
            Possemato R
  TITLE     Allosteric regulation of CAD modulates de novo pyrimidine synthesis 
            during the cell cycle.
  JOURNAL   Nat Metab. 2023 Feb 6. doi: 10.1038/s42255-023-00735-9.
  PUBMED    36747088
REFERENCE   2  (bases 1 to 12335)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Metab. 
            2023 Feb 6. doi: 10.1038/s42255-023-00735-9."
COMMENT     Sequence Label: pMXS-IRES-BLAST flag-CAD T456A S1406A
FEATURES             Location/Qualifiers
     source          1..12335
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     104..126
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     142..158
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    184..558
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     496..512
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     regulatory      585..594
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7262..7285
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    join(7351..7925,7926..7928,7929..7937)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7518..7535)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7745..7764
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7938..8333
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MVPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             8504..8973
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(9103..9120)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9274..9862)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9354..9373)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10026..10817,10818..10886))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10649..10668
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10887..10991)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             11021..11614
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    11677..12034
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             join(12093..12335,1..174)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
ORIGIN
        1 atcgtgacct gggaagcctt ggcttttgac ccccctccct gggtcaagcc ctttgtacac
       61 cctaagcctc cgcctcctct tcctccatcc gccccgtctc tcccccttga acctcctcgt
      121 tcgaccccgc ctcgatcctc cctttatcca gccctcactc cttctctagg cgcccccata
      181 tggccatatg agatcttata tggggcaccc ccgccccttg taaacttccc tgaccctgac
      241 atgacaagag ttactaacag cccctctctc caagctcact tacaggctct ctacttagtc
      301 cagcacgaag tctggagacc tctggcggca gcctaccaag aacaactgga ccgaccggtg
      361 gtacctcacc cttaccgagt cggcgacaca gtgtgggtcc gccgacacca gactaagaac
      421 ctagaacctc gctggaaagg accttacaca gtcctgctga ccacccccac cgccctcaaa
      481 gtagacggca tcgcagcttg gatacacgcc gcccacgtga aggctgccga ccccgggggt
      541 ggaccatcct ctagactgcc ggatctagct agttaattaa ggatccatgg cggccctagt
      601 gttggaggac gggtcggtcc tgcggggcca gccctttggg gccgccgtgt cgactgccgg
      661 ggaagtggtg tttcaaaccg gcatggtcgg ctaccccgag gccctcactg atccctccta
      721 caaggcacag atcttagtgc tcacctatcc tctgatcggc aactatggca tccccccaga
      781 tgaaatggat gagttcggtc tctgcaagtg gtttgaatcc tcgggcatcc acgtagcagc
      841 actggtagtg ggagagtgct gtcctactcc cagccactgg agtgccaccc gcaccctgca
      901 tgagtggctg cagcagcatg gcatccctgg cttgcaagga gtagacactc gggagctgac
      961 caagaagttg cgggaacagg ggtctctgct ggggaagctg gtccagaatg gaacagaacc
     1021 ttcaagtctg ccattcttgg accccaatgc ccgccccctg gtaccagagg tctccattaa
     1081 gactccacgg gtattcaata cagggggtgc ccctcggatc cttgctttgg actgtggcct
     1141 caagtataat cagatccgat gcctctgcca gcgtggggct gaggtcactg tggtaccctg
     1201 ggaccatgca ctagacagcc aagagtatga gggtctcttc ttaagtaatg ggcctggtga
     1261 ccctgcctcc tatcccagtg tcgtatccac actgagccgt gttttatctg agcctaatcc
     1321 ccgacctgtc tttgggatct gcctgggaca ccagctattg gccttagcca ttggggccaa
     1381 gacttacaag atgagatatg ggaaccgagg ccataaccag ccctgcttgt tggtgggctc
     1441 tgggcgctgc tttctgacat cccagaacca tgggtttgct gtggagacag actcactgcc
     1501 agcagactgg gctcctctct tcaccaacgc caatgatggt tccaatgaag gcattgtgca
     1561 caacagcttg cctttcttca gtgtccagtt tcacccagag caccaagctg gcccttcaga
     1621 tatggaactg cttttcgata tctttctgga aactgtgaaa gaggccacag ctgggaaccc
     1681 tgggggccag acagttagag agcggctgac tgagcgcctc tgtccccctg ggattcccac
     1741 tcccggctct ggacttccac caccacgaaa ggttctgatc ctgggctcag ggggcctctc
     1801 cattggccaa gctggagaat ttgactactc gggctctcag gcaattaagg ccctgaagga
     1861 ggaaaacatc cagacgttgc tgatcaaccc caatattgcc acagtgcaga cctcccaggg
     1921 gctggccgac aaggtctatt ttcttcccat agcacctcat tatgtaaccc aggtgatacg
     1981 taatgaacgc cccgatggtg tgttactgac ttttgggggc cagactgctc tgaactgtgg
     2041 tgtggagctg accaaggccg gggtgctggc tcggtatggg gtccgggtcc tgggcacacc
     2101 agtggagacc attgagctga ccgaggatcg acgggccttt gctgccagaa tggcagagat
     2161 cggagagcat gtggccccga gcgaggcagc aaattctctt gaacaggccc aggcagccgc
     2221 tgaacggctg gggtaccctg tgctagtgcg tgcagccttt gccctgggtg gcctgggctc
     2281 tggctttgcc tctaacaggg aggagctctc tgctctcgtg gccccagctt ttgcccatac
     2341 cagccaagtg ctagtagaca agtctctgaa gggatggaag gagattgagt acgaggtggt
     2401 gagagacgcc tatggcaact gtgtcacggt gtgtaacatg gagaacttgg acccactggg
     2461 catccacact ggtgagtcca tagtggtggc ccctagccag acactgaatg acagggagta
     2521 tcagctcctg aggcagacag ctatcaaggt gacccagcac ctgggaattg ttggggagtg
     2581 caatgtgcag tatgccttga accctgagtc tgagcagtat tacatcattg aagtgaatgc
     2641 caggctctct cgcagctctg ccctggccag taaggccaca ggttatccac tggcttatgt
     2701 ggcagccaag ctagcattgg gcatcccttt gcctgagctc aggaactctg tgacaggggg
     2761 tacagcagcc tttgaaccca gcgtggatta ttgtgtggtg aagattcctc gatgggacct
     2821 tagcaagttc ctgcgagtca gcacaaagat tgggagctgc atgaagagcg ttggtgaagt
     2881 catgggcatt gggcgttcat ttgaggaggc cttccagaag gccctgcgca tggtggatga
     2941 gaactgtgtg ggctttgatc acacagtgaa accagtcagc gatatggagt tggagactcc
     3001 aacagataag cggatttttg tggtggcagc tgctttgtgg gctggttatt cagtggaccg
     3061 cctgtatgag ctcacacgca tcgaccgctg gttcctgcac cgaatgaagc gtatcatcgc
     3121 acatgcccag ctgctagaac aacaccgtgg acagcctttg ccgccagacc tgctgcaaca
     3181 ggccaagtgt cttggcttct cagacaaaca gattgccctt gcagttctga gcacagagct
     3241 ggctgttcgc aagctgcgtc aggaactggg gatctgtcca gcagtgaaac agattgacac
     3301 agttgcagct gagtggccag cccagacaaa ttacctatac ctaacgtatt ggggcaccac
     3361 ccatgacctc acctttcgaa cacctcatgt cctagtcctt ggctctggcg tctaccgtat
     3421 tggctctagc gttgaatttg actggtgtgc tgtaggctgc atccagcagc tccgaaagat
     3481 gggatataag accatcatgg tgaactataa cccagagaca gtcagcaccg actatgacat
     3541 gtgtgatcga ctctactttg atgagatctc ttttgaggtg gtgatggaca tctatgagct
     3601 cgagaaccct gaaggtgtga tcctatccat gggtggacag ctgcccaaca acatggccat
     3661 ggcgttgcat cggcagcagt gccgggtgct gggcacctcc cctgaagcca ttgactcggc
     3721 tgagaaccgt ttcaagtttt cccggctcct tgacaccatt ggtatcagcc agcctcagtg
     3781 gagggagctc agtgacctcg agtctgctcg ccaattctgc cagaccgtgg ggtacccctg
     3841 tgtggtgcgc ccctcctatg tgctgagcgg tgctgctatg aatgtggcct acacggatgg
     3901 agacctggag cgcttcctga gcagcgcagc agccgtctcc aaagagcatc ccgtggtcat
     3961 ctccaagttc atccaggagg ctaaggagat tgacgtggat gccgtggcct ctgatggtgt
     4021 ggtggcagcc atcgccatct ctgagcatgt ggagaatgca ggtgtgcatt caggtgatgc
     4081 gacgctggtg acccccccac aagatatcac tgccaaaacc ctggagcgga tcaaagccat
     4141 tgtgcatgct gtgggccagg agctacaggt cacaggaccc ttcaatctgc agctcattgc
     4201 caaggatgac cagctgaaag ttattgaatg caacgtacgt gtctctcgct ccttcccctt
     4261 cgtttccaag acactgggtg tggacctagt agccttggcc acgcgggtca tcatggggga
     4321 agaagtggaa cctgtggggc taatgactgg ttctggagtt gtgggagtaa aggtgcctca
     4381 gttctccttc tcccgcttgg cgggtgctga cgtggtgttg ggtgtggaaa tgaccagtac
     4441 tggggaggtg gccggctttg gggagagccg ctgtgaggca tacctcaagg ccatgctaag
     4501 cactggcttt aagatcccca agaagaatat cctgctgacc attggcagct ataagaacaa
     4561 aagcgagctg ctcccaactg tgcggctact ggagagcctg ggctacagcc tctatgccag
     4621 tctcggcaca gctgacttct acactgagca tggcgtcaag gtaacagctg tggactggca
     4681 ctttgaggag gctgtggatg gtgagtgccc accacagcgg agcatcctgg agcagctagc
     4741 tgagaaaaac tttgagctgg tgattaacct gtcaatgcgt ggagctgggg gccggcgtct
     4801 cgcttccttt gtcaccaagg gctaccgcac ccgacgcttg gccgctgact tctccgtgcc
     4861 cctaatcatc gatatcaagt gcaccaaact ctttgtggag gccctaggcc agatcgggcc
     4921 agcccctcct ttgaaggtgc atgttgactg tatgacctcc caaaagcttg tgcgactgcc
     4981 gggattgatt gatgtccatg tgcacctgcg ggaaccaggt gggacacata aggaggactt
     5041 tgcttcaggc acagccgctg ccctggctgg gggtatcacc atggtgtgtg ccatgcctaa
     5101 tacccggccc cccatcattg acgcccctgc tctggccctg gcccagaagc tggcagaggc
     5161 tggcgcccgg tgcgactttg cgctattcct tggggcctcg tctgaaaatg caggaacctt
     5221 gggcaccgtg gccgggtctg cagccgggct gaagctttac ctcaatgaga ccttctctga
     5281 gctgcggctg gacagcgtgg tccagtggat ggagcatttc gagacatggc cctcccacct
     5341 ccccattgtg gctcacgcgg agcagcaaac cgtggctgct gtcctcatgg tggctcagct
     5401 cactcagcgc tcagtgcaca tatgtcacgt ggcacggaag gaggagatcc tgctaattaa
     5461 agctgcaaag gcacggggct tgccagtgac ctgcgaggtg gctccccacc acctgttcct
     5521 aagccatgat gacctggagc gcctggggcc tgggaagggg gaggtccggc ctgagcttgg
     5581 ctcccgccag gatgtggaag ccctgtggga gaacatggct gtcatcgact gctttgcctc
     5641 agaccatgct ccccatacct tggaggagaa gtgtgggtcc aggcccccac ctgggttccc
     5701 agggttagag accatgctgc cactactcct gacggctgta agcgagggcc ggctcagcct
     5761 ggacgacctg ctgcagcgat tgcaccacaa tcctcggcgc atctttcacc tgcctccgca
     5821 ggaggacacc tatgtggagg tggatctgga gcatgagtgg acaattccca gccacatgcc
     5881 cttctccaag gcccactgga caccttttga agggcagaaa gtgaagggca ccgtccgccg
     5941 tgtggtcctg cgaggggagg ttgcctatat cgatgggcag gttctggtac ccccgggcta
     6001 tggacaggat gtacggaagt ggccacaggg ggctgttcct cagctcccac cctcagcccc
     6061 tgccactagt gagatgacca cgacacctga aagaccccgc cgtggcatcc cagggcttcc
     6121 tgatggccgc ttccatctgc cgccccgaat ccatcgagcc tccgacccag gtttgccagc
     6181 tgaggagcca aaggagaagt cctctcggaa ggtagccgag ccagagctga tgggaacccc
     6241 tgatggcacc tgctaccctc caccaccagt accgagacag gcatctcccc agaacctggg
     6301 gacccctggc ttgctgcacc cccagacctc acccctgctg cactcattag tgggccaaca
     6361 tatcctgtcc gtccagcagt tcaccaagga tcagatgtct cacctgttca atgtggcaca
     6421 cacactgcgt atgatggtgc agaaggagcg gagcctcgac atcctgaagg ggaaggtcat
     6481 ggcctccatg ttctatgaag tgagcacacg gaccagcagc tcctttgcag cagccatggc
     6541 ccggctggga ggtgctgtgc tcagcttctc ggaagccaca tcgtccgtcc agaagggcga
     6601 atccctggct gactccgtgc agaccatgag ctgctatgcc gacgtcgtcg tgctccggca
     6661 cccccagcct ggagcagtgg agctggccgc caagcactgc cggaggccag tgatcaatgc
     6721 tggggatggg gtcggagagc accccaccca ggccctgctg gacatcttca ccatccgtga
     6781 ggagctggga actgtcaatg gcatgacgat cacgatggtg ggtgacctga agcacggacg
     6841 cacagtacat tccctggcct gcctgctcac ccagtatcgt gtcagcctgc gctacgtggc
     6901 acctcccagc ctgcgcatgc cacccactgt gcgggccttc gtggcctccc gcggcaccaa
     6961 gcaggaggaa ttcgagagca ttgaggaggc gctgcctgac actgatgtgc tctacatgac
     7021 tcgaatccag aaggaacgat ttggctctac ccaggagtac gaagcttgct ttggtcagtt
     7081 catcctcact ccccacatca tgacccgggc caagaagaag atggtggtga tgcacccgat
     7141 gccccgtgtc aacgagataa gcgtggaagt ggactcggat ccccgcgcag cctacttccg
     7201 ccaggctgag aacggcatgt acatccgcat ggctctgtta gccaccgtgc tgggccgttt
     7261 cgactacaaa gacgatgacg acaagtagga attcctgcag gcctcgaggg ccggcgcgcc
     7321 gcggccgcta cgtaaattcc gccccccccc cccctctccc tccccccccc ctaacgttac
     7381 tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat
     7441 attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat
     7501 tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga
     7561 agcagttcct ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca
     7621 gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac
     7681 acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt
     7741 caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca
     7801 ttgtatggga tctgatctgg ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt
     7861 aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg
     7921 ataatatggc cacaaccatg gttcctttgt ctcaagaaga atccaccctc attgaaagag
     7981 caacggctac aatcaacagc atccccatct ctgaagacta cagcgtcgcc agcgcagctc
     8041 tctctagcga cggccgcatc ttcactggtg tcaatgtata tcattttact gggggacctt
     8101 gtgcagaact cgtggtgctg ggcactgctg ctgctgcggc agctggcaac ctgacttgta
     8161 tcgtcgcgat cggaaatgag aacaggggca tcttgagccc ctgcggacgg tgccgacagg
     8221 tgcttctcga tctgcatcct gggatcaaag ccatagtgaa ggacagtgat ggacagccga
     8281 cggcagttgg gattcgtgaa ttgctgccct ctggttatgt gtgggagggc taagtcgacg
     8341 ataaaataaa agattttatt tagtctccag aaaaaggggg gaatgaaaga ccccacctgt
     8401 aggtttggca agctagctta agtaacgcca ttttgcaagg catggaaaaa tacataactg
     8461 agaatagaga agttcagatc aaggtcagga acagatggaa cagctgaata tgggccaaac
     8521 aggatatctg tggtaagcag ttcctgcccc ggctcagggc caagaacaga tggaacagct
     8581 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga
     8641 acagatggtc cccagatgcg gtccagccct cagcagtttc tagagaacca tcagatgttt
     8701 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc
     8761 gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc
     8821 ctcactcggg gcgccagtcc tccgattgac tgagtcgccc gggtacccgt gtatccaata
     8881 aaccctcttg cagttgcatc cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg
     8941 agtgattgac tacccgtcag cgggggtctt tcacatgcag catgtatcaa aattaatttg
     9001 gttttttttc ttaagtattt acattaaatg gccatagttg cattaatgaa tcggccaacg
     9061 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     9121 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     9181 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     9241 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     9301 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     9361 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     9421 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     9481 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     9541 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     9601 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     9661 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     9721 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     9781 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     9841 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     9901 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     9961 ctagatcctt ttgcggccgg ccgcaaatca atctaaagta tatatgagta aacttggtct
    10021 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
    10081 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
    10141 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
    10201 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
    10261 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
    10321 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
    10381 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
    10441 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
    10501 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
    10561 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
    10621 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
    10681 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
    10741 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
    10801 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
    10861 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
    10921 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
    10981 ggggttccgc gcacatttcc ccgaaaagtg ccacctgcat aatgaaagac cccacctgta
    11041 ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc atggaaaaat acataactga
    11101 gaatagaaaa gttcagatca aggtcaggaa cagatggaac agctgaatat gggccaaaca
    11161 ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggaacagctg
    11221 aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa
    11281 cagatggtcc ccagatgcgg tccagccctc agcagtttct agagaaccat cagatgtttc
    11341 cagggtgccc caaggacctg aaatgaccct gtgccttatt tgaactaacc aatcagttcg
    11401 cttctcgctt ctgttcgcgc gcttctgctc cccgagctca ataaaagagc ccacaacccc
    11461 tcactcggcg cgccagtcct ccgattgact gagtcgcccg ggtacccgtg tatccaataa
    11521 accctcttgc agttgcatcc gacttgtggt ctcgctgttc cttgggaggg tctcctctga
    11581 gtgattgact acccgtcagc gggggtcttt catttggggg ctcgtccggg atcgggagac
    11641 ccctgcccag ggaccaccga cccaccaccg ggaggtaagc tggccagcaa cttatctgtg
    11701 tctgtccgat tgtctagtgt ctatgactga ttttatgcgc ctgcgtcggt actagttagc
    11761 taactagctc tgtatctggc ggacccgtgg tggaactgac gagttcggaa cacccggccg
    11821 caaccctggg agacgtccca gggacttcgg gggccgtttt tgtggcccga cctgagtcca
    11881 aaaatcccga tcgttttgga ctctttggtg caccccccta ataggaggga tatgtggttc
    11941 tggtaggaga cgagaaccta aaacagttcc cgcctccgtc tgaatttttg ctttcggttt
    12001 gggaccgaag ccgcgccgcg cgtcttgtct gctgcagcat cgttctgtgt tgtctctgtc
    12061 tgactgtgtt tctgtatttg tctgaaaatt agggccagac tgttaccact cccttaagtt
    12121 tgaccttagg tcactggaaa gatgtcgagc ggatcgctca caaccagtcg gtagatgtca
    12181 agaagagacg ttgggttacc ttctgctctg cagaatggcc aacctttaac gtcggatggc
    12241 cgcgagacgg cacctttaac cgagacctca tcacccaggt taagatcaag gtcttttcac
    12301 ctggcccgca tggacaccca gaccaggtcc cctac
//
