LOCUS       sequence-33349-e         827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Cox RS 3rd, Dunlop MJ, Elowitz MB
  TITLE     A synthetic three-color scaffold for monitoring genetic regulation 
            and noise.
  JOURNAL   J Biol Eng. 2010 Jul 21;4:10. doi: 10.1186/1754-1611-4-10.
  PUBMED    20646328
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2010-07-21"; volume: "4:10. doi"; pages: " 
            10.1186/1754-1611-4-10"
COMMENT     Sequence Label: pZS2-123
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    287..306
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    320..336
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             377..397
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 tcttgttcaa tcatgcgaaa cgatcctcat cctgtctctt gatcagatct tgatcccctg
       61 cgccatcaga tccttggcgg caagaaagcc atccagttta ctttgcaggg cttcccaacc
      121 ttaccagagg gcgccccagc tggcaattcc gacgtctaag aaaccattag ctagtcctgt
      181 tctagctagc tagacatgca accaccggtc gcttggactc ctgttgatat ccccccgggg
      241 ggaacatagc atttttatcc ataagattag cggatctaac ctttacaatt gtgagcgctc
      301 acaattatga tagattcaat tgtgagcgga taacaatttc acacagacct aggcttgaga
      361 aaatctagaa ataattttgt ttaactttaa gaaggagcac ctgccatggt ttccaagggc
      421 gaggaggata acatggctat cattaaagag ttcatgcgct tcaaagttca catggagggt
      481 tctgttaacg gtcacgagtt cgagatcgaa ggcgaaggcg agggccgtcc gtatgaaggc
      541 acccagaccg ccaaactgaa agtgactaaa ggcggcccgc tgccttttgc gtgggacatc
      601 ctgagcccgc aatttatgta cggttctaaa gcgtatgtta aacacccagc ggatatcccg
      661 gactatctga agctgtcttt tccggaaggt ttcaagtggg aacgcgtaat gaattttgaa
      721 gatggtggtg tcgtgaccgt cactcaggac tcctccctgc aggatggcga gttcatctat
      781 aaagttaaac tgcgtggtac taattttcca tctgatggcc cggtgat
//
