LOCUS       sequence-333505-        4184 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human MDM2 [NM_002392.5] without stop codon 
            (for C-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4184)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 4184)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E130 Hs.MDM2-nostop
FEATURES             Location/Qualifiers
     source          1..4184
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..23
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     15..32
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     16..32
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    62..161
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1653..1752)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2129..2920
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3013..3601
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3502..3521
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3755..3772
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3931..3958)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4050..4136)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 cccagtcacg acgttgtaaa acgacggcca gtcttaaaat tcgagctcgg tccgaggatc
       61 cgaaataatg attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgataagca
      121 atgctttttt ataatgccaa ctttgtacaa aaaagttggc accatggtga ggagcaggca
      181 aatgtgcaat accaacatgt ctgtacctac tgatggtgct gtaaccacct cacagattcc
      241 agcttcggaa caagagaccc tggttagacc aaagccattg cttttgaagt tattaaagtc
      301 tgttggtgca caaaaagaca cttatactat gaaagaggtt cttttttatc ttggccagta
      361 tattatgact aaacgattat atgatgagaa gcaacaacat attgtatatt gttcaaatga
      421 tcttctagga gatttgtttg gcgtgccaag cttctctgtg aaagagcaca ggaaaatata
      481 taccatgatc tacaggaact tggtagtagt caatcagcag gaatcatcgg actcaggtac
      541 atctgtgagt gagaacaggt gtcaccttga aggtgggagt gatcaaaagg accttgtaca
      601 agagcttcag gaagagaaac cttcatcttc acatttggtt tctagaccat ctacctcatc
      661 tagaaggaga gcaattagtg agacagaaga aaattcagat gaattatctg gtgaacgaca
      721 aagaaaacgc cacaaatctg atagtatttc cctttccttt gatgaaagcc tggctctgtg
      781 tgtaataagg gagatatgtt gtgaaagaag cagtagcagt gaatctacag ggacgccatc
      841 gaatccggat cttgatgctg gtgtaagtga acattcaggt gattggttgg atcaggattc
      901 agtttcagat cagtttagtg tagaatttga agttgaatct ctcgactcag aagattatag
      961 ccttagtgaa gaaggacaag aactctcaga tgaagatgat gaggtatatc aagttactgt
     1021 gtatcaggca ggggagagtg atacagattc atttgaagaa gatcctgaaa tttccttagc
     1081 tgactattgg aaatgcactt catgcaatga aatgaatccc ccccttccat cacattgcaa
     1141 cagatgttgg gcccttcgtg agaattggct tcctgaagat aaagggaaag ataaagggga
     1201 aatctctgag aaagccaaac tggaaaactc aacacaagct gaagagggct ttgatgttcc
     1261 tgattgtaaa aaaactatag tgaatgattc cagagagtca tgtgttgaag aaaatgatga
     1321 taaaattaca caagcttcac aatcacaaga aagtgaagac tattctcagc catcaacttc
     1381 tagtagcatt atttatagca gccaagaaga tgtgaaagag tttgaaaggg aagaaaccca
     1441 agacaaagaa gagagtgtgg aatctagttt gccccttaat gccattgaac cttgtgtgat
     1501 ttgtcaaggt cgacctaaaa atggttgcat tgtccatggc aaaacaggac atcttatggc
     1561 ctgctttaca tgtgcaaaga agctaaagaa aaggaataag ccctgcccag tatgtagaca
     1621 accaattcaa atgattgtgc taacttattt cccctcaact ttcttgtaca aagttggcat
     1681 tataggaaag cattgcttat caatttgttg caacgaacag gtcactatca gtcaaaataa
     1741 aatcattatt tgcatgcaag cttagatcct ctagaccagc caggacagaa atgcctcgac
     1801 ttcgctgcta cccaaggttg ccgggtgacg cacaccgtgg aaacggatga aggcacgaac
     1861 ccagtggaca taagcctgtt cggttcgtaa gctgtaatgc aagtagcgta tgcgctcacg
     1921 caactggtcc agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat
     1981 ggcttgttat gactgttttt ttggggtaca gtctatgcct cgggcatcca agcagcaagc
     2041 gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc aacgatgtta cgcagcaggg
     2101 cagtcgccct aaaacaaagt taaacattat gagggaagcg gtgatcgccg aagtatcgac
     2161 tcaactatca gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt
     2221 acatttgtac ggctccgcag tggatggcgg cctgaagcca cacagtgata ttgatttgct
     2281 ggttacggtg accgtaaggc ttgatgaaac aacgcggcga gctttgatca acgacctttt
     2341 ggaaacttcg gcttcccctg gagagagcga gattctccgc gctgtagaag tcaccattgt
     2401 tgtgcacgac gacatcattc cgtggcgtta tccagctaag cgcgaactgc aatttggaga
     2461 atggcagcgc aatgacattc ttgcaggtat cttcgagcca gccacgatcg acattgatct
     2521 ggctatcttg ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc cagcggcgga
     2581 ggaactcttt gatccggttc ctgaacagga tctatttgag gcgctaaatg aaaccttaac
     2641 gctatggaac tcgccgcccg actgggctgg cgatgagcga aatgtagtgc ttacgttgtc
     2701 ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg
     2761 ggcaatggag cgcctgccgg cccagtatca gcccgtcata cttgaagcta gacaggctta
     2821 tcttggacaa gaagaagatc gcttggcctc gcgcgcagat cagttggaag aatttgtcca
     2881 ctacgtgaaa ggcgagatca ccaaggtagt cggcaaataa ccctcgagcc acccatgacc
     2941 aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat
     3001 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     3061 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     3121 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     3181 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     3241 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     3301 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     3361 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     3421 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     3481 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     3541 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     3601 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     3661 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     3721 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     3781 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     3841 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atacgcgtac
     3901 cgctagccag gaagagtttg tagaaacgca aaaaggccat ccgtcaggat ggccttctgc
     3961 ttagtttgat gcctggcagt ttatggcggg cgtcctgccc gccaccctcc gggccgttgc
     4021 ttcacaacgt tcaaatccgc tcccggcgga tttgtcctac tcaggagagc gttcaccgac
     4081 aaacaacaga taaaacgaaa ggcccagtct tccgactgag cctttcgttt tatttgatgc
     4141 ctggcagttc cctactctcg cgttaacgct agcatggatg tttt
//
