LOCUS       sequence-33351-e         583 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 583)
  AUTHORS   Gallo CM, Munro E, Rasoloson D, Merritt C, Seydoux G
  TITLE     Processing bodies and germ granules are distinct RNA granules that 
            interact in C. elegans embryos.
  JOURNAL   Dev Biol. 2008 Nov 1. 323(1):76-87.
  PUBMED    18692039
REFERENCE   2  (bases 1 to 583)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Biol. 
            2008 Nov 1. 323(1):76-87."
COMMENT     Sequence Label: pCG26
FEATURES             Location/Qualifiers
     source          1..583
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             58..78
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             91..135
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     protein_bind    142..166
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gaactataca aatgcccggg ggatcggtgg agctccaccg gtggcggccg ctctagagag
       61 aatctttatt ttcagggcgc cgccaaattc aaagaaaccg ctgctgctaa attcgaacgc
      121 cagcacatgg acagcggatc aacaagtttg tacaaaaaag caggcttaat gagcgacgct
      181 aaaaaaaagg ccgccgccga gctagccgcg aaaaacctcg cacaactcca gaaaatcgac
      241 atcgccgcca gcaagattct cgacaagatg ccatttgccg cgatttatca tatcgatgcg
      301 gcgagaaaag agtggaatca gtcgaattgc gaaggaacat ttttcgtcta tcagagagct
      361 gatcggccat attttagttt cctcatcgca aatcgcaacg atccatcgga tttcatcgag
      421 ccgctcaccc tcaaccatat tctccgccac gacggaaatt tcatctactt ttacaaggat
      481 ttggcctcaa ttcaagcact atggttccac cagattgatg atgctcagaa aatctacaat
      541 ttgttgcaaa aactcgtgaa tcgtctgaaa ggaagcacta cgg
//
