LOCUS       sequence-33352-e         721 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 721)
  AUTHORS   Gallo CM, Munro E, Rasoloson D, Merritt C, Seydoux G
  TITLE     Processing bodies and germ granules are distinct RNA granules that 
            interact in C. elegans embryos.
  JOURNAL   Dev Biol. 2008 Nov 1. 323(1):76-87.
  PUBMED    18692039
REFERENCE   2  (bases 1 to 721)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Biol. 
            2008 Nov 1. 323(1):76-87."
COMMENT     Sequence Label: pCG31
FEATURES             Location/Qualifiers
     source          1..721
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             55..75
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             88..132
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     protein_bind    139..163
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(544..568)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ctatacaaat gcccggggga tcggtggagc tccaccggtg gcggccgctc tagagagaat
       61 ctttattttc agggcgccgc caaattcaaa gaaaccgctg ctgctaaatt cgaacgccag
      121 cacatggaca gcggatcaac aagtttgtac aaaaaagcag gcttaatgga cttgcccgat
      181 ccctatttac ccggagcaat aagcttgttc gagcagcttg acaaaaaatt attagttgtt
      241 ctccgcgacg gacggaaatt gattggcttc ttgagatcga tagatcaatt tgccaatctt
      301 atattggaag atgtcgtaga acgcacattt gttgagaaat acttctgtga aacgggtcaa
      361 ggattcatgc ttattcgcgg agagaatgtt gagcttgctg gagaaatcga tgataccatt
      421 gagactggac ttacgcaggt gtctcccgaa gaatttcgtc ggctggagga tgagtatatt
      481 gccaaaaatc cgccaaagtt cctaaaacga caagcagaga aaacagaaga acacgatgca
      541 tgaacccagc tttcttgtac aaagtggttg atttgttttg tatatttatc cactcacccc
      601 ctctctttgt cctgtgaatg aacttgtgcc aaaaaagcca aaaataattt acttttttta
      661 aagacatatt aaaacttgaa aacttggaac aatacggtgc gaaagcgaaa cagagacaga
      721 a
//
